P81435 (SERC_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine aminotransferase
EC=2.6.1.52

Alternative name(s):
Phosphohydroxythreonine aminotransferase
Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	BUsg_302

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity. HAMAP-Rule MF_00160
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP-Rule MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP-Rule MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP-Rule MF_00160
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP-Rule MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP-Rule MF_00160
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00160
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00160.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Phosphoserine aminotransferase HAMAP-Rule MF_00160		PRO_0000150159
Regions
	Region	76 – 77	2	Pyridoxal phosphate binding By similarity
	Region	238 – 239	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	42	1	L-glutamate By similarity
	Binding site	102	1	Pyridoxal phosphate By similarity
	Binding site	153	1	Pyridoxal phosphate By similarity
	Binding site	173	1	Pyridoxal phosphate By similarity
	Binding site	196	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	197	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81435 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F30153652525047D	FASTA	361	41,261
	10 20 30 40 50 60 MNLIYNFSAG PAMIPKDVLY QAKKELQNWN QIGCSIMEIS HRSKEFIQVA LEAEQDLRDL 70 80 90 100 110 120 LNISNSYEIL FCQGGARGQF AAVPMNLLGN FKETDYINSG YWSNCALIEA KKYCIPKNIS 130 140 150 160 170 180 VRQKKNGKSF LLKPSQWNIS DNSAYIHYCP NETIDGMSIY EEPNFKNKII VGDFSSFILS 190 200 210 220 230 240 RRINIENYGL IYAGAQKNIG PSGITIILIR KDLIGYASKI SPSIFNYYII SKYNSMFNTP 250 260 270 280 290 300 PTFSWYLSGL VFKWLKKQGG IKKIEQLNQK KSNLLYQIID NSNFYINDID KRNRSQMNVV 310 320 330 340 350 360 FHLYNSKLDN LFLKEAKNAG LNALKGHNVI GGMRASIYNA MPLEGVQSLA KFMLYFEKKY G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA." Thao M.L., Baumann P. Curr. Microbiol. 35:68-69(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43549 Genomic DNA. Translation: AAC05435.1. AE013218 Genomic DNA. Translation: AAM67856.1.
RefSeq	NP_660645.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P81435.
SMR	P81435. Positions 4-361.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg302.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67856; AAM67856; BUsg_302.
GeneID	1005506.
KEGG	bas:BUsg302.
PATRIC	21247411. VBIBucAph100086_0315.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1932.
KO	K00831.
OMA	MSIMEMS.
ProtClustDB	PRK05355.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-316-MONOMER.
UniPathway	UPA00135; UER00197. UPA00244; UER00311.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
HAMAP	MF_00160. SerC_aminotrans_5.
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR022278. Pser_aminoTfrase. IPR003248. Pser_aminoTfrase_subgr. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR21152:SF1. PTHR21152:SF1. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000525. SerC. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_BUCAP

Accession

Primary (citable) accession number: P81435

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program