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P81434

- SYS_BUCAP

UniProt

P81434 - SYS_BUCAP

Protein

Serine--tRNA ligase

Gene

serS

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (30 Aug 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).UniRule annotation

    Catalytic activityi

    ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).UniRule annotation
    ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei287 – 2871SerineUniRule annotation
    Binding sitei387 – 3871SerineUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi264 – 2663ATPUniRule annotation
    Nucleotide bindingi351 – 3544ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. serine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. selenocysteine biosynthetic process Source: UniProtKB-HAMAP
    2. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
    3. seryl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-317-MONOMER.
    UniPathwayiUPA00906; UER00895.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine--tRNA ligaseUniRule annotation (EC:6.1.1.11UniRule annotation)
    Alternative name(s):
    Seryl-tRNA synthetaseUniRule annotation
    Short name:
    SerRSUniRule annotation
    Seryl-tRNA(Ser/Sec) synthetaseUniRule annotation
    Gene namesi
    Name:serSUniRule annotation
    Ordered Locus Names:BUsg_303
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Serine--tRNA ligasePRO_0000122019Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. The tRNA molecule binds across the dimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi198804.BUsg303.

    Structurei

    3D structure databases

    ProteinModelPortaliP81434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni233 – 2353Serine bindingUniRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi371 – 3766Poly-Lys

    Domaini

    Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0172.
    KOiK01875.
    OMAiAIAMSVP.
    OrthoDBiEOG61KBH9.

    Family and domain databases

    Gene3Di1.10.287.40. 1 hit.
    HAMAPiMF_00176. Ser_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR002317. Ser-tRNA-ligase_type_1.
    IPR015866. Ser-tRNA-synth_1_N.
    IPR010978. tRNA-bd_arm.
    [Graphical view]
    PANTHERiPTHR11778. PTHR11778. 1 hit.
    PfamiPF02403. Seryl_tRNA_N. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
    PRINTSiPR00981. TRNASYNTHSER.
    SUPFAMiSSF46589. SSF46589. 1 hit.
    TIGRFAMsiTIGR00414. serS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P81434-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLNPYLLRNQ IDAISKKLLK KKFKLDISLI SSLEKKRKKL QIKTDNLQYK    50
    HNTLSALFKK EKKIQELDEN LKRTLTKSSK NLSELKIELN LLQEKIHNFS 100
    LSIPNIPSDD VPEGNSSENN KIVKYWGKKR EYSFAVRDHV EIGNKLNQLD 150
    WKSSAKISGA RFIVMKGNIA LLHRALSQFM LDLHTIKHGY TETYVPYLVN 200
    HDSLYGTGQL PKFTDDLFHI NSIDKKKYVL IPTAEVPLTN LFSNQILNET 250
    ELPIMLTAHT PCFRSEASSY GRDSKGLIRL HQFDKVELVQ IIQPELSMNA 300
    LELLTHHAEK VLQLLELPYR KVLLCGGEMG FSATKTYDLE VWFPSQKKYR 350
    EISSCSNMSD FQARRMKTRY KKKKEKSNSF VHTLNGSGLA IGRTLAAILE 400
    NYQDSDGRVT IPKILQKKYM QGIEYIN 427
    Length:427
    Mass (Da):49,238
    Last modified:August 30, 2002 - v2
    Checksum:iCC416EFD7C861B87
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451D → E in AAC05434. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43549 Genomic DNA. Translation: AAC05434.1.
    AE013218 Genomic DNA. Translation: AAM67857.1.
    RefSeqiNP_660646.1. NC_004061.1.
    WP_011053824.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67857; AAM67857; BUsg_303.
    GeneIDi1005507.
    KEGGibas:BUsg303.
    PATRICi21247413. VBIBucAph100086_0316.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43549 Genomic DNA. Translation: AAC05434.1 .
    AE013218 Genomic DNA. Translation: AAM67857.1 .
    RefSeqi NP_660646.1. NC_004061.1.
    WP_011053824.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali P81434.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg303.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67857 ; AAM67857 ; BUsg_303 .
    GeneIDi 1005507.
    KEGGi bas:BUsg303.
    PATRICi 21247413. VBIBucAph100086_0316.

    Phylogenomic databases

    eggNOGi COG0172.
    KOi K01875.
    OMAi AIAMSVP.
    OrthoDBi EOG61KBH9.

    Enzyme and pathway databases

    UniPathwayi UPA00906 ; UER00895 .
    BioCyci BAPH198804:GHMG-317-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.40. 1 hit.
    HAMAPi MF_00176. Ser_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR002317. Ser-tRNA-ligase_type_1.
    IPR015866. Ser-tRNA-synth_1_N.
    IPR010978. tRNA-bd_arm.
    [Graphical view ]
    PANTHERi PTHR11778. PTHR11778. 1 hit.
    Pfami PF02403. Seryl_tRNA_N. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
    PRINTSi PR00981. TRNASYNTHSER.
    SUPFAMi SSF46589. SSF46589. 1 hit.
    TIGRFAMsi TIGR00414. serS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA."
      Thao M.L., Baumann P.
      Curr. Microbiol. 35:68-69(1997)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiSYS_BUCAP
    AccessioniPrimary (citable) accession number: P81434
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 30, 2002
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3