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Protein

Serine--tRNA ligase

Gene

serS

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).UniRule annotation

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).UniRule annotation
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).UniRule annotation

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Serine--tRNA ligase (serS)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei287SerineUniRule annotation1
Binding sitei387SerineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi264 – 266ATPUniRule annotation3
Nucleotide bindingi351 – 354ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-320-MONOMER
UniPathwayiUPA00906; UER00895

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligaseUniRule annotation (EC:6.1.1.11UniRule annotation)
Alternative name(s):
Seryl-tRNA synthetaseUniRule annotation
Short name:
SerRSUniRule annotation
Seryl-tRNA(Ser/Sec) synthetaseUniRule annotation
Gene namesi
Name:serSUniRule annotation
Ordered Locus Names:BUsg_303
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001220191 – 427Serine--tRNA ligaseAdd BLAST427

Proteomic databases

PRIDEiP81434

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg303

Structurei

3D structure databases

ProteinModelPortaliP81434
SMRiP81434
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni233 – 235Serine bindingUniRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi371 – 376Poly-Lys6

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGR Bacteria
COG0172 LUCA
KOiK01875
OMAiSPCFRRE
OrthoDBiPOG091H01YY

Family and domain databases

CDDicd00770 SerRS_core, 1 hit
HAMAPiMF_00176 Ser_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR002317 Ser-tRNA-ligase_type_1
IPR015866 Ser-tRNA-synth_1_N
IPR033729 SerRS_core
IPR010978 tRNA-bd_arm
PANTHERiPTHR43697 PTHR43697, 1 hit
PfamiView protein in Pfam
PF02403 Seryl_tRNA_N, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PIRSFiPIRSF001529 Ser-tRNA-synth_IIa, 1 hit
PRINTSiPR00981 TRNASYNTHSER
SUPFAMiSSF46589 SSF46589, 1 hit
TIGRFAMsiTIGR00414 serS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

P81434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNPYLLRNQ IDAISKKLLK KKFKLDISLI SSLEKKRKKL QIKTDNLQYK
60 70 80 90 100
HNTLSALFKK EKKIQELDEN LKRTLTKSSK NLSELKIELN LLQEKIHNFS
110 120 130 140 150
LSIPNIPSDD VPEGNSSENN KIVKYWGKKR EYSFAVRDHV EIGNKLNQLD
160 170 180 190 200
WKSSAKISGA RFIVMKGNIA LLHRALSQFM LDLHTIKHGY TETYVPYLVN
210 220 230 240 250
HDSLYGTGQL PKFTDDLFHI NSIDKKKYVL IPTAEVPLTN LFSNQILNET
260 270 280 290 300
ELPIMLTAHT PCFRSEASSY GRDSKGLIRL HQFDKVELVQ IIQPELSMNA
310 320 330 340 350
LELLTHHAEK VLQLLELPYR KVLLCGGEMG FSATKTYDLE VWFPSQKKYR
360 370 380 390 400
EISSCSNMSD FQARRMKTRY KKKKEKSNSF VHTLNGSGLA IGRTLAAILE
410 420
NYQDSDGRVT IPKILQKKYM QGIEYIN
Length:427
Mass (Da):49,238
Last modified:August 30, 2002 - v2
Checksum:iCC416EFD7C861B87
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45D → E in AAC05434 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43549 Genomic DNA Translation: AAC05434.1
AE013218 Genomic DNA Translation: AAM67857.1
RefSeqiWP_011053824.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM67857; AAM67857; BUsg_303
KEGGibas:BUsg_303

Similar proteinsi

Entry informationi

Entry nameiSYS_BUCAP
AccessioniPrimary (citable) accession number: P81434
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 30, 2002
Last modified: May 23, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

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