P81432 (SYD_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BUsg_306

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP-Rule MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Aspartate--tRNA ligase HAMAP-Rule MF_00044_B		PRO_0000110844
Regions
	Compositional bias	79 – 84	6	Poly-Asn HAMAP-Rule MF_00044_B

Sequences

	Sequence		Length	Mass (Da)
	P81432 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F318DFA1654780E7	FASTA	584	67,824
	10 20 30 40 50 60 MRTKYCGNIR IIDLHKSVIL CGWVHKIRNF SQFIFIDMRD WTGIVQLVFE KKNNKVFTKA 70 80 90 100 110 120 VNLKNESCIQ VIGIVKKRNA NNNNLDTGEI EILVNKIKVF NISKNLPLDY SNNSNDDIRL 130 140 150 160 170 180 KYRYLDLRRS ELLENLKIRN KITHLIRIFM ENKNFLDIET PFLTKSTPEG ARDYLVPSRN 190 200 210 220 230 240 YPGNFYALPQ SPQLFKQILM ISGIDKYYQI VKCFRDEDLR SDRQPEFTQI DIEASFVSST 250 260 270 280 290 300 KIRNLVETLI KKIWLKVINY NLNKFPKISF YDSMKRYGSD KPDLRNPMEI VDISDIVIEE 310 320 330 340 350 360 KVASFFQINL KKKNRIALLC FGQGNKISQK KIDEYSNYVK KFGAKKLFYI KINKIENRFQ 370 380 390 400 410 420 DIQSSIKNIL DKNTLENILR KTNAKNGNIL FLLADEEKIV NKSLGMLRIK LGNDFIFFKK 430 440 450 460 470 480 NTWKPVWIVD FPMFKQNSDG KFSSNHHPFT ALKKNNQNKL EKNPNLAISD SYDLVINGYE 490 500 510 520 530 540 IGGGSVRIHD AKIQKKVFNI IGIEKQFQRE KFGFLIEALK YGPPPHAGIA LGLDRIVMLL 550 560 570 580 TNTNNIRDVI AFPKTTSANC LMTDSPSKLK KSILNELGIN ILKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA." Thao M.L., Baumann P. Curr. Microbiol. 35:68-69(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43549 Genomic DNA. Translation: AAC05432.1. AE013218 Genomic DNA. Translation: AAM67860.1.
RefSeq	NP_660649.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P81432.
SMR	P81432. Positions 1-583.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg306.
Proteomic databases
PRIDE	P81432.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67860; AAM67860; BUsg_306.
GeneID	1005510.
KEGG	bas:BUsg306.
PATRIC	21247419. VBIBucAph100086_0319.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
KO	K01876.
OMA	QEWAKQR.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-320-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.1360.30. 1 hit.
HAMAP	MF_00044_B. Asp_tRNA_synth_B.
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-ligase_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. PTHR22594. 1 hit. PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BUCAP

Accession

Primary (citable) accession number: P81432

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	May 29, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program