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P81432

- SYD_BUCAP

UniProt

P81432 - SYD_BUCAP

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Protein

Aspartate--tRNA ligase

Gene

aspS

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Asp) in a two-step reaction: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei169 – 1691AspartateUniRule annotation
Binding sitei215 – 2151AspartateUniRule annotation
Binding sitei224 – 2241ATPUniRule annotation
Binding sitei446 – 4461AspartateUniRule annotation
Binding sitei480 – 4801ATPUniRule annotation
Binding sitei487 – 4871AspartateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi215 – 2173ATPUniRule annotation
Nucleotide bindingi532 – 5354ATPUniRule annotation

GO - Molecular functioni

  1. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:BUsg_306
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Aspartate--tRNA ligasePRO_0000110844Add
BLAST

Proteomic databases

PRIDEiP81432.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg306.

Structurei

3D structure databases

ProteinModelPortaliP81432.
SMRiP81432. Positions 1-583.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 1964AspartateUniRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi79 – 846Poly-Asn

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0173.
KOiK01876.
OMAiSLSRKQI.
OrthoDBiEOG68Q0NX.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P81432-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTKYCGNIR IIDLHKSVIL CGWVHKIRNF SQFIFIDMRD WTGIVQLVFE
60 70 80 90 100
KKNNKVFTKA VNLKNESCIQ VIGIVKKRNA NNNNLDTGEI EILVNKIKVF
110 120 130 140 150
NISKNLPLDY SNNSNDDIRL KYRYLDLRRS ELLENLKIRN KITHLIRIFM
160 170 180 190 200
ENKNFLDIET PFLTKSTPEG ARDYLVPSRN YPGNFYALPQ SPQLFKQILM
210 220 230 240 250
ISGIDKYYQI VKCFRDEDLR SDRQPEFTQI DIEASFVSST KIRNLVETLI
260 270 280 290 300
KKIWLKVINY NLNKFPKISF YDSMKRYGSD KPDLRNPMEI VDISDIVIEE
310 320 330 340 350
KVASFFQINL KKKNRIALLC FGQGNKISQK KIDEYSNYVK KFGAKKLFYI
360 370 380 390 400
KINKIENRFQ DIQSSIKNIL DKNTLENILR KTNAKNGNIL FLLADEEKIV
410 420 430 440 450
NKSLGMLRIK LGNDFIFFKK NTWKPVWIVD FPMFKQNSDG KFSSNHHPFT
460 470 480 490 500
ALKKNNQNKL EKNPNLAISD SYDLVINGYE IGGGSVRIHD AKIQKKVFNI
510 520 530 540 550
IGIEKQFQRE KFGFLIEALK YGPPPHAGIA LGLDRIVMLL TNTNNIRDVI
560 570 580
AFPKTTSANC LMTDSPSKLK KSILNELGIN ILKK
Length:584
Mass (Da):67,824
Last modified:August 1, 1998 - v1
Checksum:iF318DFA1654780E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43549 Genomic DNA. Translation: AAC05432.1.
AE013218 Genomic DNA. Translation: AAM67860.1.
RefSeqiNP_660649.1. NC_004061.1.
WP_011053827.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67860; AAM67860; BUsg_306.
GeneIDi1005510.
KEGGibas:BUsg306.
PATRICi21247419. VBIBucAph100086_0319.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43549 Genomic DNA. Translation: AAC05432.1 .
AE013218 Genomic DNA. Translation: AAM67860.1 .
RefSeqi NP_660649.1. NC_004061.1.
WP_011053827.1. NC_004061.1.

3D structure databases

ProteinModelPortali P81432.
SMRi P81432. Positions 1-583.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg306.

Proteomic databases

PRIDEi P81432.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67860 ; AAM67860 ; BUsg_306 .
GeneIDi 1005510.
KEGGi bas:BUsg306.
PATRICi 21247419. VBIBucAph100086_0319.

Phylogenomic databases

eggNOGi COG0173.
KOi K01876.
OMAi SLSRKQI.
OrthoDBi EOG68Q0NX.

Enzyme and pathway databases

BioCyci BAPH198804:GHMG-320-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPi MF_00044. Asp_tRNA_synth.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfami PF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR01042. TRNASYNTHASP.
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA."
    Thao M.L., Baumann P.
    Curr. Microbiol. 35:68-69(1997)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiSYD_BUCAP
AccessioniPrimary (citable) accession number: P81432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3