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P81432 (SYD_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartate--tRNA ligase

EC=6.1.1.12
Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS
Gene names
Name:aspS
Ordered Locus Names:BUsg_306
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP-Rule MF_00044_B

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00044_B

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00044_B.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processaspartyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aspartate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 584584Aspartate--tRNA ligase HAMAP-Rule MF_00044_B
PRO_0000110844

Regions

Compositional bias79 – 846Poly-Asn HAMAP-Rule MF_00044_B

Sequences

Sequence LengthMass (Da)Tools
P81432 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: F318DFA1654780E7

FASTA58467,824
        10         20         30         40         50         60 
MRTKYCGNIR IIDLHKSVIL CGWVHKIRNF SQFIFIDMRD WTGIVQLVFE KKNNKVFTKA 

        70         80         90        100        110        120 
VNLKNESCIQ VIGIVKKRNA NNNNLDTGEI EILVNKIKVF NISKNLPLDY SNNSNDDIRL 

       130        140        150        160        170        180 
KYRYLDLRRS ELLENLKIRN KITHLIRIFM ENKNFLDIET PFLTKSTPEG ARDYLVPSRN 

       190        200        210        220        230        240 
YPGNFYALPQ SPQLFKQILM ISGIDKYYQI VKCFRDEDLR SDRQPEFTQI DIEASFVSST 

       250        260        270        280        290        300 
KIRNLVETLI KKIWLKVINY NLNKFPKISF YDSMKRYGSD KPDLRNPMEI VDISDIVIEE 

       310        320        330        340        350        360 
KVASFFQINL KKKNRIALLC FGQGNKISQK KIDEYSNYVK KFGAKKLFYI KINKIENRFQ 

       370        380        390        400        410        420 
DIQSSIKNIL DKNTLENILR KTNAKNGNIL FLLADEEKIV NKSLGMLRIK LGNDFIFFKK 

       430        440        450        460        470        480 
NTWKPVWIVD FPMFKQNSDG KFSSNHHPFT ALKKNNQNKL EKNPNLAISD SYDLVINGYE 

       490        500        510        520        530        540 
IGGGSVRIHD AKIQKKVFNI IGIEKQFQRE KFGFLIEALK YGPPPHAGIA LGLDRIVMLL 

       550        560        570        580 
TNTNNIRDVI AFPKTTSANC LMTDSPSKLK KSILNELGIN ILKK 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA."
Thao M.L., Baumann P.
Curr. Microbiol. 35:68-69(1997)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43549 Genomic DNA. Translation: AAC05432.1.
AE013218 Genomic DNA. Translation: AAM67860.1.
RefSeqNP_660649.1. NC_004061.1.

3D structure databases

ProteinModelPortalP81432.
SMRP81432. Positions 1-583.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg306.

Proteomic databases

PRIDEP81432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67860; AAM67860; BUsg_306.
GeneID1005510.
KEGGbas:BUsg306.
PATRIC21247419. VBIBucAph100086_0319.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0173.
KOK01876.
OMAQLDVEMS.
OrthoDBEOG68Q0NX.
ProtClustDBPRK00476.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-320-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPMF_00044_B. Asp_tRNA_synth_B.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsTIGR00459. aspS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYD_BUCAP
AccessionPrimary (citable) accession number: P81432
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries