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P81432

- SYD_BUCAP

UniProt

P81432 - SYD_BUCAP

Protein

Aspartate--tRNA ligase

Gene

aspS

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the attachment of glutamate to tRNA(Asp) in a two-step reaction: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).UniRule annotation

    Catalytic activityi

    ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei169 – 1691AspartateUniRule annotation
    Binding sitei215 – 2151AspartateUniRule annotation
    Binding sitei224 – 2241ATPUniRule annotation
    Binding sitei446 – 4461AspartateUniRule annotation
    Binding sitei480 – 4801ATPUniRule annotation
    Binding sitei487 – 4871AspartateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi215 – 2173ATPUniRule annotation
    Nucleotide bindingi532 – 5354ATPUniRule annotation

    GO - Molecular functioni

    1. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-320-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation)
    Alternative name(s):
    Aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    AspRSUniRule annotation
    Gene namesi
    Name:aspSUniRule annotation
    Ordered Locus Names:BUsg_306
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Aspartate--tRNA ligasePRO_0000110844Add
    BLAST

    Proteomic databases

    PRIDEiP81432.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi198804.BUsg306.

    Structurei

    3D structure databases

    ProteinModelPortaliP81432.
    SMRiP81432. Positions 1-583.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni193 – 1964AspartateUniRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi79 – 846Poly-Asn

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0173.
    KOiK01876.
    OMAiSLSRKQI.
    OrthoDBiEOG68Q0NX.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPiMF_00044. Asp_tRNA_synth.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    PfamiPF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P81432-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTKYCGNIR IIDLHKSVIL CGWVHKIRNF SQFIFIDMRD WTGIVQLVFE    50
    KKNNKVFTKA VNLKNESCIQ VIGIVKKRNA NNNNLDTGEI EILVNKIKVF 100
    NISKNLPLDY SNNSNDDIRL KYRYLDLRRS ELLENLKIRN KITHLIRIFM 150
    ENKNFLDIET PFLTKSTPEG ARDYLVPSRN YPGNFYALPQ SPQLFKQILM 200
    ISGIDKYYQI VKCFRDEDLR SDRQPEFTQI DIEASFVSST KIRNLVETLI 250
    KKIWLKVINY NLNKFPKISF YDSMKRYGSD KPDLRNPMEI VDISDIVIEE 300
    KVASFFQINL KKKNRIALLC FGQGNKISQK KIDEYSNYVK KFGAKKLFYI 350
    KINKIENRFQ DIQSSIKNIL DKNTLENILR KTNAKNGNIL FLLADEEKIV 400
    NKSLGMLRIK LGNDFIFFKK NTWKPVWIVD FPMFKQNSDG KFSSNHHPFT 450
    ALKKNNQNKL EKNPNLAISD SYDLVINGYE IGGGSVRIHD AKIQKKVFNI 500
    IGIEKQFQRE KFGFLIEALK YGPPPHAGIA LGLDRIVMLL TNTNNIRDVI 550
    AFPKTTSANC LMTDSPSKLK KSILNELGIN ILKK 584
    Length:584
    Mass (Da):67,824
    Last modified:August 1, 1998 - v1
    Checksum:iF318DFA1654780E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43549 Genomic DNA. Translation: AAC05432.1.
    AE013218 Genomic DNA. Translation: AAM67860.1.
    RefSeqiNP_660649.1. NC_004061.1.
    WP_011053827.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67860; AAM67860; BUsg_306.
    GeneIDi1005510.
    KEGGibas:BUsg306.
    PATRICi21247419. VBIBucAph100086_0319.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43549 Genomic DNA. Translation: AAC05432.1 .
    AE013218 Genomic DNA. Translation: AAM67860.1 .
    RefSeqi NP_660649.1. NC_004061.1.
    WP_011053827.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali P81432.
    SMRi P81432. Positions 1-583.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg306.

    Proteomic databases

    PRIDEi P81432.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67860 ; AAM67860 ; BUsg_306 .
    GeneIDi 1005510.
    KEGGi bas:BUsg306.
    PATRICi 21247419. VBIBucAph100086_0319.

    Phylogenomic databases

    eggNOGi COG0173.
    KOi K01876.
    OMAi SLSRKQI.
    OrthoDBi EOG68Q0NX.

    Enzyme and pathway databases

    BioCyci BAPH198804:GHMG-320-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPi MF_00044. Asp_tRNA_synth.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    Pfami PF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR01042. TRNASYNTHASP.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA."
      Thao M.L., Baumann P.
      Curr. Microbiol. 35:68-69(1997)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiSYD_BUCAP
    AccessioniPrimary (citable) accession number: P81432
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3