Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P81425

- DPP4_BOVIN

UniProt

P81425 - DPP4_BOVIN

Protein

Dipeptidyl peptidase 4

Gene

DPP4

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (26 Jul 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.By similarity

    Catalytic activityi

    Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.PROSITE-ProRule annotation

    Enzyme regulationi

    Inhibited by GPC3 and diprotin A.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei629 – 6291Charge relay systemPROSITE-ProRule annotation
    Active sitei707 – 7071Charge relay systemPROSITE-ProRule annotation
    Active sitei739 – 7391Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. dipeptidyl-peptidase activity Source: UniProtKB
    2. protease binding Source: UniProtKB
    3. protein homodimerization activity Source: UniProtKB
    4. receptor binding Source: UniProtKB
    5. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. endothelial cell migration Source: UniProtKB
    3. negative regulation of extracellular matrix disassembly Source: UniProtKB
    4. positive regulation of cell proliferation Source: UniProtKB
    5. T cell costimulation Source: UniProtKB

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease, Receptor, Serine protease

    Keywords - Biological processi

    Cell adhesion

    Protein family/group databases

    MEROPSiS09.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dipeptidyl peptidase 4 (EC:3.4.14.5)
    Alternative name(s):
    Activation molecule 3
    Short name:
    ACT3
    Adenosine deaminase complexing protein
    Short name:
    ADCP-I
    Dipeptidyl peptidase IV
    Short name:
    DPP IV
    T-cell activation antigen CD26
    WC10
    CD_antigen: CD26
    Cleaved into the following 2 chains:
    Alternative name(s):
    Dipeptidyl peptidase IV membrane form
    Alternative name(s):
    Dipeptidyl peptidase IV soluble form
    Gene namesi
    Name:DPP4
    Synonyms:CD26
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Chain Dipeptidyl peptidase 4 soluble form : Secreted
    Note: Detected in the serum and the seminal fluid.By similarity
    Cell membrane By similarity; Single-pass type II membrane protein By similarity. Apical cell membrane By similarity; Single-pass type II membrane protein By similarity. Cell projectioninvadopodium membrane By similarity; Single-pass type II membrane protein By similarity. Cell projectionlamellipodium membrane By similarity; Single-pass type II membrane protein By similarity. Cell junction By similarity. Membrane raft By similarity
    Note: Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. cell junction Source: UniProtKB-SubCell
    3. cell surface Source: UniProtKB
    4. endocytic vesicle Source: UniProtKB
    5. extracellular region Source: UniProtKB-SubCell
    6. integral component of membrane Source: UniProtKB-KW
    7. invadopodium membrane Source: UniProtKB
    8. lamellipodium Source: UniProtKB
    9. lamellipodium membrane Source: UniProtKB-SubCell
    10. membrane raft Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 765765Dipeptidyl peptidase 4 membrane formPRO_0000027209Add
    BLAST
    Chaini38 – 765728Dipeptidyl peptidase 4 soluble formBy similarityPRO_0000027210Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi84 – 841N-linked (GlcNAc...)By similarity
    Glycosylationi91 – 911N-linked (GlcNAc...)By similarity
    Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi218 – 2181N-linked (GlcNAc...)By similarity
    Glycosylationi228 – 2281N-linked (GlcNAc...)By similarity
    Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi280 – 2801N-linked (GlcNAc...)By similarity
    Glycosylationi320 – 3201N-linked (GlcNAc...)By similarity
    Disulfide bondi384 ↔ 393By similarity
    Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi443 ↔ 446By similarity
    Disulfide bondi453 ↔ 471By similarity
    Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi648 ↔ 761By similarity
    Glycosylationi684 – 6841N-linked (GlcNAc...)By similarity

    Post-translational modificationi

    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.By similarity
    N- and O-Glycosylated.By similarity
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP81425.

    Expressioni

    Tissue specificityi

    Intestinal epithelium, dendritic cells and several immune system tissues.

    Interactioni

    Subunit structurei

    Monomer. Heterodimer with Seprase (FAP). Requires homodimerization for optimal dipeptidyl peptidase activity and T-cell costimulation. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Associates with collagen. Interacts with PTPRC; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Interacts (extracellular domain) with ADA; does not inhibit its dipeptidyl peptidase activity. Interacts with CAV1 (via the N-terminus); the interaction is direct. Interacts (via cytoplasmic tail) with CARD11 (via PDZ domain); its homodimerization is necessary for interaction with CARD11. Interacts with IGF2R; the interaction is direct. Interacts with GPC3 By similarity. Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP81425.
    SMRiP81425. Positions 38-764.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini30 – 765736ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2923Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9B family. DPPIV subfamily.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1506.
    HOGENOMiHOG000231875.
    HOVERGENiHBG005527.
    InParanoidiP81425.
    KOiK01278.

    Family and domain databases

    Gene3Di2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view]
    PfamiPF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P81425-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTPWKVLLG LLAIAALVTV ITVPVVLLTK GNDASTDSRR TYTLADYLKN    50
    TFRMKFYNLR WVSDHEYLYK QENNILLFNA EYGNSSIFLE NSTFDEFGHS 100
    INDYSVSPDR QYILFEYNYV KQWRHSYTAS YDIYDLNKRQ LITEERIPNN 150
    TQWITWSSVG HKLAYVWNND IYVKNEPNSP SQRITWTGKK DVIYNGITDW 200
    VYEEEVFSAY SALWWSPNST FLAYAQFNDT EVPLIEYSFY SDESLQYPKT 250
    VKIPYPKAGA VNPTIKFFVV NISSLSPNIN ATSQQIVPPG SVLIGDHYLC 300
    DVTWVTEERI SLQWLRRIQN YSIMDICDYD RSTGRWISSV GRQHIEISTT 350
    GWVGRFRPAE PHFTSDGNSF YKIISNEEGY KHICHFQTDK RNCTFITKGA 400
    WEVIGIEALT SDYLYYISNE YKGMPGARNL YKIQLNDYTK VTCLSCELNP 450
    DRCQYYSVSF SQEAKYYQLR CSGPGLPLYT LHNSNNDKEL RVLENNSDLD 500
    QVLQDVQMPS KKLDFIHLHG TKFWYQMILP PHFDKSKKYP LLLEVYAGPC 550
    SQKADAIFRL NWATYLASTE NIIVASFDGR GSGYQGDKIM HAINRRLGTF 600
    EVEDQIEATR QFSKMGFVDD KRIAIWGWSY GGYVTSMVLG AGSGVFKCGI 650
    AVAPVSKWEY YDSVYTERYM GLPTPEDNLD SYRNSTVMSR AENFKQVEYL 700
    LIHGTADDNV HFQQSAQISK ALVDAGVDFQ SMWYTDEDHG IASSTAHQHI 750
    YTHMSHFLKQ CFSLL 765
    Length:765
    Mass (Da):88,369
    Last modified:July 26, 2002 - v3
    Checksum:iE32165421F43E116
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF461806 mRNA. Translation: AAL67836.1.
    AY056834 mRNA. Translation: AAL23628.1.
    BC102523 mRNA. Translation: AAI02524.1.
    RefSeqiNP_776464.1. NM_174039.2.
    UniGeneiBt.8282.

    Genome annotation databases

    GeneIDi281122.
    KEGGibta:281122.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF461806 mRNA. Translation: AAL67836.1 .
    AY056834 mRNA. Translation: AAL23628.1 .
    BC102523 mRNA. Translation: AAI02524.1 .
    RefSeqi NP_776464.1. NM_174039.2.
    UniGenei Bt.8282.

    3D structure databases

    ProteinModelPortali P81425.
    SMRi P81425. Positions 38-764.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P81425.
    ChEMBLi CHEMBL2559.

    Protein family/group databases

    MEROPSi S09.003.

    Proteomic databases

    PRIDEi P81425.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281122.
    KEGGi bta:281122.

    Organism-specific databases

    CTDi 1803.

    Phylogenomic databases

    eggNOGi COG1506.
    HOGENOMi HOG000231875.
    HOVERGENi HBG005527.
    InParanoidi P81425.
    KOi K01278.

    Miscellaneous databases

    NextBioi 20805190.

    Family and domain databases

    Gene3Di 2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view ]
    Pfami PF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of bovine CD26 upregulated by a staphylococcal superantigen."
      Lee S.-U., Park Y.-H., Davis W.C., Hamilton M.J., Naessens J., Bohach G.A.
      Immunogenetics 54:216-220(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lymphocyte.
    2. "CD26 is expressed on a restricted subpopulation of dendritic cells in vivo."
      Gliddon D.R., Howard C.J.
      Eur. J. Immunol. 32:1472-1481(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-17.
      Tissue: Thymus.
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.
    4. "Identity of activation molecule 3 on superantigen-stimulated bovine cells is CD26."
      Lee S.-U., Ferens W., Davis W.C., Hamilton M.J., Park Y.-H., Fox L.K., Naessens J., Bohach G.A.
      Infect. Immun. 69:7190-7193(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-24.
      Tissue: T-cell.
    5. "The CP-I subunit of adenosine deaminase complexing protein from calf kidney is identical to human, mouse, and rat dipeptidyl peptidase IV."
      Ben-Shooshan I., Parola A.H.
      Comp. Biochem. Physiol. 119B:289-292(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 537-546.
      Tissue: Kidney.

    Entry informationi

    Entry nameiDPP4_BOVIN
    AccessioniPrimary (citable) accession number: P81425
    Secondary accession number(s): Q3ZCC2, Q8WMG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 26, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3