P81425 (DPP4_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified June 11, 2014. Version 108. History...
Names and origin
|Protein names||Recommended name:|
Dipeptidyl peptidase 4
Activation molecule 3
Adenosine deaminase complexing protein
Dipeptidyl peptidase IV
Short name=DPP IV
T-cell activation antigen CD26
|Organism||Bos taurus (Bovine) [Reference proteome]|
|Taxonomic identifier||9913 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos|
|Sequence length||765 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Inhibited by GPC3 and diprotin A By similarity.
Monomer. Heterodimer with Seprase (FAP). Requires homodimerization for optimal dipeptidyl peptidase activity and T-cell costimulation. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Associates with collagen. Interacts with PTPRC; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Interacts (extracellular domain) with ADA; does not inhibit its dipeptidyl peptidase activity. Interacts with CAV1 (via the N-terminus); the interaction is direct. Interacts (via cytoplasmic tail) with CARD11 (via PDZ domain); its homodimerization is necessary for interaction with CARD11. Interacts with IGF2R; the interaction is direct. Interacts with GPC3 By similarity. Homodimer.
Cell membrane; Single-pass type II membrane protein By similarity. Apical cell membrane; Single-pass type II membrane protein By similarity. Cell projection › invadopodium membrane; Single-pass type II membrane protein By similarity. Cell projection › lamellipodium membrane; Single-pass type II membrane protein By similarity. Cell junction By similarity. Membrane raft By similarity. Note: Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface By similarity.
Intestinal epithelium, dendritic cells and several immune system tissues.
The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing By similarity.
N- and O-Glycosylated By similarity.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation By similarity.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 765||765||Dipeptidyl peptidase 4 membrane form||PRO_0000027209|
|Chain||38 – 765||728||Dipeptidyl peptidase 4 soluble form By similarity||PRO_0000027210|
|Topological domain||1 – 6||6||Cytoplasmic Potential|
|Transmembrane||7 – 29||23||Helical; Signal-anchor for type II membrane protein; Potential|
|Topological domain||30 – 765||736||Extracellular Potential|
|Active site||629||1||Charge relay system By similarity|
|Active site||707||1||Charge relay system By similarity|
|Active site||739||1||Charge relay system By similarity|
Amino acid modifications
|Glycosylation||84||1||N-linked (GlcNAc...) By similarity|
|Glycosylation||91||1||N-linked (GlcNAc...) By similarity|
|Glycosylation||149||1||N-linked (GlcNAc...) Potential|
|Glycosylation||218||1||N-linked (GlcNAc...) By similarity|
|Glycosylation||228||1||N-linked (GlcNAc...) By similarity|
|Glycosylation||271||1||N-linked (GlcNAc...) Potential|
|Glycosylation||280||1||N-linked (GlcNAc...) By similarity|
|Glycosylation||320||1||N-linked (GlcNAc...) By similarity|
|Glycosylation||392||1||N-linked (GlcNAc...) Potential|
|Glycosylation||495||1||N-linked (GlcNAc...) Potential|
|Glycosylation||684||1||N-linked (GlcNAc...) By similarity|
|Disulfide bond||384 ↔ 393||By similarity|
|Disulfide bond||443 ↔ 446||By similarity|
|Disulfide bond||453 ↔ 471||By similarity|
|Disulfide bond||648 ↔ 761||By similarity|
|||"Molecular characterization of bovine CD26 upregulated by a staphylococcal superantigen."|
Lee S.-U., Park Y.-H., Davis W.C., Hamilton M.J., Naessens J., Bohach G.A.
Immunogenetics 54:216-220(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"CD26 is expressed on a restricted subpopulation of dendritic cells in vivo."|
Gliddon D.R., Howard C.J.
Eur. J. Immunol. 32:1472-1481(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-17.
|||NIH - Mammalian Gene Collection (MGC) project|
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
|||"Identity of activation molecule 3 on superantigen-stimulated bovine cells is CD26."|
Lee S.-U., Ferens W., Davis W.C., Hamilton M.J., Park Y.-H., Fox L.K., Naessens J., Bohach G.A.
Infect. Immun. 69:7190-7193(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-24.
|||"The CP-I subunit of adenosine deaminase complexing protein from calf kidney is identical to human, mouse, and rat dipeptidyl peptidase IV."|
Ben-Shooshan I., Parola A.H.
Comp. Biochem. Physiol. 119B:289-292(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 537-546.
|AF461806 mRNA. Translation: AAL67836.1.|
AY056834 mRNA. Translation: AAL23628.1.
BC102523 mRNA. Translation: AAI02524.1.
|RefSeq||NP_776464.1. NM_174039.2. |
3D structure databases
|SMR||P81425. Positions 38-764. |
Protein family/group databases
Protocols and materials databases
Genome annotation databases
Family and domain databases
|Gene3D||18.104.22.168. 1 hit. |
22.214.171.1240. 1 hit.
|InterPro||IPR029058. AB_hydrolase. |
|Pfam||PF00930. DPPIV_N. 1 hit. |
PF00326. Peptidase_S9. 1 hit.
|SUPFAM||SSF53474. SSF53474. 1 hit. |
|PROSITE||PS00708. PRO_ENDOPEP_SER. 1 hit. |
|Accession||Primary (citable) accession number: P81425|
Secondary accession number(s): Q3ZCC2, Q8WMG8
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|