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Protein

Antimicrobial peptide 1

Gene
N/A
Organism
Phytolacca americana (American pokeweed) (Phytolacca decandra)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Possesses antifungal activity.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Antimicrobial peptide 1
Alternative name(s):
AFPS-1
Anti-fungal protein 1
PAFP-S
Pa-AMP-1
Short name:
AMP1
OrganismiPhytolacca americana (American pokeweed) (Phytolacca decandra)
Taxonomic identifieri3527 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesPhytolaccaceaePhytolacca

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27272 PublicationsAdd
BLAST
Chaini28 – 6538Antimicrobial peptide 1PRO_0000001312Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 47
Disulfide bondi37 ↔ 51
Disulfide bondi46 ↔ 62

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Seed specific.1 Publication

Structurei

Secondary structure

1
65
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 376Combined sources
Beta strandi47 – 548Combined sources
Turni55 – 584Combined sources
Beta strandi59 – 635Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DKCNMR-A28-65[»]
ProteinModelPortaliP81418.
SMRiP81418. Positions 28-64.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81418.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the AMP family.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiIPR013006. Antimicrobial_C6_CS.
IPR009101. Gurmarin/antifun_pep.
IPR024206. Gurmarin/antimicrobial_peptd.
[Graphical view]
PfamiPF11410. Antifungal_pept. 1 hit.
[Graphical view]
SUPFAMiSSF57048. SSF57048. 1 hit.
PROSITEiPS60011. PLANT_C6_AMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVSSAYLK FALVMILLLS VISAVMSAGC IKNGGRCNAS AGPPYCCSSY
60
CFQIAGQSYG VCKNR
Length:65
Mass (Da):6,804
Last modified:May 30, 2000 - v1
Checksum:i0073DE3ABBDC5B5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048745 mRNA. Translation: AAC05129.1.
AF105062 Genomic DNA. Translation: AAD17942.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048745 mRNA. Translation: AAC05129.1.
AF105062 Genomic DNA. Translation: AAD17942.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DKCNMR-A28-65[»]
ProteinModelPortaliP81418.
SMRiP81418. Positions 28-64.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP81418.

Family and domain databases

InterProiIPR013006. Antimicrobial_C6_CS.
IPR009101. Gurmarin/antifun_pep.
IPR024206. Gurmarin/antimicrobial_peptd.
[Graphical view]
PfamiPF11410. Antifungal_pept. 1 hit.
[Graphical view]
SUPFAMiSSF57048. SSF57048. 1 hit.
PROSITEiPS60011. PLANT_C6_AMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification, characterization, and molecular cloning of the gene of a seed-specific antimicrobial protein from pokeweed."
    Liu Y., Luo J., Xu C., Ren F., Peng C., Wu G., Zhao J.
    Plant Physiol. 122:1015-1024(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 28-65, TISSUE SPECIFICITY.
    Tissue: Seed.
  2. Feng S.
    Submitted (JUN-1998) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 28-65.
    Tissue: Seed.
  3. "Solution structure of PAFP-S: a new knottin-type antifungal peptide from the seeds of Phytolacca americana."
    Gao G.-H., Liu W., Dai J.-X., Wang J.-F., Hu Z., Zhang Y., Wang D.-C.
    Biochemistry 40:10973-10978(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 28-65.

Entry informationi

Entry nameiPAFP_PHYAM
AccessioniPrimary (citable) accession number: P81418
Secondary accession number(s): O82728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: December 9, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.