P81409 (DP2L_PYRFU) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 79.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: DNA polymerase II large subunit Short name=Pol II EC=2.7.7.7 Alternative name(s): DP2 | ||||
| Gene names |
| ||||
| Organism | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP] | ||||
| Taxonomic identifier | 186497 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus ›  |
Protein attributes
| Sequence length | 1263 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase. HAMAP-Rule MF_00324 |
| Catalytic activity | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP-Rule MF_00324 |
| Subunit structure | Heterodimer of a large subunit and a small subunit. |
| Sequence similarities | Belongs to the archaeal DNA polymerase II family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA replication |
| Ligand | DNA-binding |
| Molecular function | DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase |
| Technical term | Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replicationInferred from electronic annotation. Source: HAMAP |
| Molecular_function | 3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA bindingInferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1263 | 1263 | DNA polymerase II large subunit HAMAP-Rule MF_00324 | PRO_0000152579 | |||||
Regions | |||||||||
| Compositional bias | 1235 – 1252 | 18 | Lys-rich HAMAP-Rule MF_00324 | ||||||
Experimental info | |||||||||
| Sequence conflict | 1030 | 1 | G → R in BAA25164. Ref.1 | ||||||
| Sequence conflict | 1034 | 1 | R → G in BAA25164. Ref.1 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization." Uemori T., Sato Y., Kato I., Doi H., Ishino Y. Genes Cells 2:499-512(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1. |
| [2] | "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1. |
| [3] | "A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus." Imamura M., Uemori T., Kato I., Doi H., Ishino Y. Biol. Pharm. Bull. 18:1647-1652(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D84670 Genomic DNA. Translation: BAA25164.1. AE009950 Genomic DNA. Translation: AAL80143.1. |
| PIR | T43934. |
| RefSeq | NP_577748.2. NC_003413.1. |
3D structure databases | |
| ProteinModelPortal | P81409. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 186497.PF0019. |
Proteomic databases | |
| PRIDE | P81409. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAL80143; AAL80143; PF0019. |
| GeneID | 1467847. |
| KEGG | pfu:PF0019. |
Phylogenomic databases | |
| eggNOG | COG1933. |
| HOGENOM | HOG000286435. |
| KO | K02322. |
| OMA | RNCDGDE. |
| ProtClustDB | PRK04023. |
Family and domain databases | |
| HAMAP | MF_00324. DNApol_II_L_arch. |
| InterPro | IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view] |
| Pfam | PF03833. PolC_DP2. 1 hit. [Graphical view] |
| PIRSF | PIRSF016275. PolC_DP2. 1 hit. |
| TIGRFAMs | TIGR00354. polC. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | DP2L_PYRFU | ||||||||
| Accession | Primary (citable) accession number: P81409 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |