Reviewed, UniProtKB/Swiss-Prot P81409 (DP2L_PYRFU)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7
Alternative name(s):
    DP2

Gene names

Name:	polC
Ordered Locus Names:	PF0019

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1263	1263	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152579
Regions
	Compositional bias	1235 – 1252	18	Lys-rich HAMAP MF_00324
Experimental info
	Sequence conflict	1030	1	G → R in BAA25164. Ref.1
	Sequence conflict	1034	1	R → G in BAA25164. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P81409-1 [UniParc]. Last modified April 3, 2002. Version 2. Checksum: 3BFFB2CC8D054F4C	FASTA	1,263	143,169
	10 20 30 40 50 60 MELPKEIEEY FEMLQREIDK AYEIAKKARS QGKDPSTDVE IPQATDMAGR VESLVGPPGV 70 80 90 100 110 120 AQRIRELLKE YDKEIVALKI VDEIIEGKFG DFGSKEKYAE QAVRTALAIL TEGIVSAPLE 130 140 150 160 170 180 GIADVKIKRN TWADNSEYLA LYYAGPIRSS GGTAQALSVL VGDYVRRKLG LDRFKPSGKH 190 200 210 220 230 240 IERMVEEVDL YHRAVSRLQY HPSPDEVRLA MRNIPIEITG EATDDVEVSH RDVEGVETNQ 250 260 270 280 290 300 LRGGAILVLA EGVLQKAKKL VKYIDKMGID GWEWLKEFVE AKEKGEEIEE SESKAEESKV 310 320 330 340 350 360 ETRVEVEKGF YYKLYEKFRA EIAPSEKYAK EIIGGRPLFA GPSENGGFRL RYGRSRVSGF 370 380 390 400 410 420 ATWSINPATM VLVDEFLAIG TQMKTERPGK GAVVTPATTA EGPIVKLKDG SVVRVDDYNL 430 440 450 460 470 480 ALKIRDEVEE ILYLGDAIIA FGDFVENNQT LLPANYVEEW WIQEFVKAVN EAYEVELRPF 490 500 510 520 530 540 EENPRESVEE AAEYLEVDPE FLAKMLYDPL RVKPPVELAI HFSEILEIPL HPYYTLYWNT 550 560 570 580 590 600 VNPKDVERLW GVLKDKATIE WGTFRGIKFA KKIEISLDDL GSLKRTLELL GLPHTVREGI 610 620 630 640 650 660 VVVDYPWSAA LLTPLGNLEW EFKAKPFYTV IDIINENNQI KLRDRGISWI GARMGRPEKA 670 680 690 700 710 720 KERKMKPPVQ VLFPIGLAGG SSRDIKKAAE EGKIAEVEIA FFKCPKCGHV GPETLCPECG 730 740 750 760 770 780 IRKELIWTCP KCGAEYTNSQ AEGYSYSCPK CNVKLKPFTK RKIKPSELLN RAMENVKVYG 790 800 810 820 830 840 VDKLKGVMGM TSGWKIAEPL EKGLLRAKNE VYVFKDGTIR FDATDAPITH FRPREIGVSV 850 860 870 880 890 900 EKLRELGYTH DFEGKPLVSE DQIVELKPQD VILSKEAGKY LLRVARFVDD LLEKFYGLPR 910 920 930 940 950 960 FYNAEKMEDL IGHLVIGLAP HTSAGIVGRI IGFVDALVGY AHPYFHAAKR RNCDGDEDSV 970 980 990 1000 1010 1020 MLLLDALLNF SRYYLPEKRG GKMDAPLVIT TRLDPREVDS EVHNMDVVRY YPLEFYEATY 1030 1040 1050 1060 1070 1080 ELKSPKELVG VIERVEDRLG KPEMYYGIKF THDTDDIALG PKMSLYKQLG DMEEKVKRQL 1090 1100 1110 1120 1130 1140 TLAERIRAVD QHYVAETILN SHLIPDLRGN LRSFTRQEFR CVKCNTKYRR PPLDGKCPVC 1150 1160 1170 1180 1190 1200 GGKIVLTVSK GAIEKYLGTA KMLVANYNVK PYTRQRICLT EKDIDSLFEY LFPEAQLTLI 1210 1220 1230 1240 1250 1260 VDPNDICMKM IKERTGETVQ GGLLENFNSS GNNGKKIEKK EKKAKEKPKK KKVISLDDFF SKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization." Uemori T., Sato Y., Kato I., Doi H., Ishino Y. Genes Cells 2:499-512(1997) [PubMed: 9348040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]	"A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus." Imamura M., Uemori T., Kato I., Doi H., Ishino Y. Biol. Pharm. Bull. 18:1647-1652(1995) [PubMed: 8787781] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D84670 Genomic DNA. Translation: BAA25164.1. AE010127 Genomic DNA. Translation: AAL80143.1.
PIR	T43934.
RefSeq	NP_577748.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1467847.
GenomeReviews	Gene locus PF0019 in contig AE009950_GR.
KEGG	pfu:PF0019.
NMPDR	fig|186497.1.peg.19.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P81409.
OMA	P81409. NCDGDED.
Enzyme and pathway databases
BRENDA	2.7.7.7. 321.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_PYRFU

Accession

Primary (citable) accession number: P81409

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 3, 2002
Last modified:	May 5, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)