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P81406 (GAPN_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
EC=1.2.1.9
Alternative name(s):
Glyceraldehyde-3-phosphate dehydrogenase [NADP+]
Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase
Triosephosphate dehydrogenase
Gene names
Name:GAPN
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important as a means of generating NADPH for biosynthetic reactions. Ref.4

Catalytic activity

D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionglyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
PRO_0000056578

Regions

Nucleotide binding245 – 2495NAD By similarity
Region169 – 1702Substrate binding By similarity
Region297 – 2993Substrate binding By similarity

Sites

Active site2641Proton acceptor By similarity
Active site2981Nucleophile
Binding site1161Substrate By similarity
Binding site1921NADP By similarity
Binding site1951NADP By similarity
Binding site2301NADP By similarity
Binding site3911NADP By similarity
Binding site4511Substrate By similarity
Site1691Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
P81406 [UniParc].

Last modified December 15, 1998. Version 1.
Checksum: 52FADC2F77C613FA

FASTA49653,004
        10         20         30         40         50         60 
MAATGVLAEI IDGDVYKYYA DGEWKKSTSG KSVAIINPTT RKPQYKVQAC SQEEVNKVMD 

        70         80         90        100        110        120 
SAKSAQKSWA KTPLWKRAEL LHKAAAILKE HKAAIAECLV KEIAKPAKDA VTEVVRSGDL 

       130        140        150        160        170        180 
VSYCAEEGVR ILGEGKFLVS DSFPGNERTK YCLTSKIPLG VILAIPPFNY PVNLAVSKIA 

       190        200        210        220        230        240 
PALIAGNSIV LKPPTQGAVA ALHMVHCFHL AGFPKGLISC VTGKGSEIGD FLTMHPGVNC 

       250        260        270        280        290        300 
ISFTGGDTGI AISKKSGMIP LQMELGGKDA CIVLEDADLD LVAANIIKGG FSYSGQRCTA 

       310        320        330        340        350        360 
VKVVLVMESV ADALVEKVKV KVAKLSVGPP EDDSDITPVV SESSANFIEG LVNDAKEKGA 

       370        380        390        400        410        420 
TFCQEYKREG NLIWPLLLDN VRPDMRIAWE EPFGPVLPVI RINSVEEGIH HCNASNFGLQ 

       430        440        450        460        470        480 
GCVFTKDINK AIMISDAMES GTVQINSAPA RGPDHFPFQG IKDSGIGSQG ITNSINMMTK 

       490 
VKTTVINLPS PSYTMG

« Hide

References

[1]"Non-phosphorylating GAPDH of higher plants is a member of the aldehyde dehydrogenase superfamily with no sequence homology to phosphorylating GAPDH."
Habenicht A., Hellman U., Cerff R.
J. Mol. Biol. 237:165-171(1994) [PubMed: 7545914] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Rosakrone.
Tissue: Shoot.
[2]"First genomic cloning and characterization of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from higher plants."
Habenicht A., Weile H., Cerff R.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase: biochemistry, structure, occurrence and evolution."
Habenicht A.
Biol. Chem. 378:1413-1419(1997) [PubMed: 9461340] [Abstract]
Cited for: CARACTERIZATION.
[4]"Engineering a central metabolic pathway: glycolysis with no net phosphorylation in an Escherichia coli gap mutant complemented with a plant GapN gene."
Valverde F., Losada M., Serrano A.
FEBS Lett. 449:153-158(1999) [PubMed: 10338122] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U34988 Genomic DNA. Translation: AAO38512.1.
X75327 mRNA. Translation: CAA53076.1.
PIRS43832.

3D structure databases

ProteinModelPortalP81406.
SMRP81406. Positions 15-488.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
Gene3DG3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAPN_PEA
AccessionPrimary (citable) accession number: P81406
Secondary accession number(s): Q53WX1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: October 19, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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