P81402 (NLTP1_PRUPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Non-specific lipid-transfer protein 1 Short name=LTP 1 Alternative name(s): Allergen Pru p 1 Major allergen Pru p 3 Allergen=Pru p 3
Organism	Prunus persica (Peach) (Amygdalus persica)
Taxonomic identifier	3760 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Amygdaloideae › Amygdaleae › Prunus

Protein attributes

Sequence length	91 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE.
Sequence similarities	Belongs to the plant LTP family.

Ontologies

Keywords
Biological process	Transport
Disease	Allergen
Ligand	Lipid-binding
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid transport Inferred from electronic annotation. Source: InterPro
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 91

91

Non-specific lipid-transfer protein 1

PRO_0000153880

Amino acid modifications

Disulfide bond

3 ↔ 50

Ref.3

Disulfide bond

13 ↔ 27

Ref.3

Disulfide bond

28 ↔ 73

Ref.3

Disulfide bond

48 ↔ 87

Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

91

Helix Strand Turn

Details...

Helix

3 – 10

8

Helix

11 – 13

3

Helix

14 – 19

6

Helix

25 – 37

13

Helix

41 – 57

17

Helix

63 – 72

10

Helix

87 – 89

3

Sequences

	Sequence		Length	Mass (Da)
	P81402 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: BB84569AA9E4B332	FASTA	91	9,178
	10 20 30 40 50 60 ITCGQVSSAL APCIPYVRGG GAVPPACCNG IRNVNNLART TPDRQAACNC LKQLSASVPG 70 80 90 VNPNNAAALP GKCGVHIPYK ISASTNCATV K

References

[1]	"Complete amino acid sequence determination of the major allergen of peach (Prunus persica) Pru p 1." Pastorello E.A., Ortolani C., Baroglio C., Pravettoni V., Ispano M., Giuffrida M.G., Fortunato D., Farioli L., Monza M., Napolitano L., Sacco M., Scibola E., Conti A. Biol. Chem. 380:1315-1320(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"The major allergen of peach (Prunus persica) is a lipid transfer protein." Pastorello E.A., Farioli L., Pravettoni V., Ortolani C., Ispano M., Monza M., Baroglio C., Scibola E., Ansaloni R., Incorvaia C., Conti A. J. Allergy Clin. Immunol. 103:520-526(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-32; 53-66 AND 73-80.
[3]	"Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens." Pasquato N., Berni R., Folli C., Folloni S., Cianci M., Pantano S., Helliwell J.R., Zanotti G. J. Mol. Biol. 356:684-694(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), DISULFIDE BONDS.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ALG	X-ray	2.30	A/B	1-91	[»]
2B5S	X-ray	2.35	A/B	1-91	[»]

ProteinModelPortal

P81402.

SMR

P81402. Positions 1-91.

ModBase

Search...

Protein family/group databases

Allergome

3454. Pru p 3.0101.
603. Pru p 3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR000528. Plant_LTP.
[Graphical view]

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

PRINTS

PR00382. LIPIDTRNSFER.

SMART

SM00499. AAI. 1 hit.
[Graphical view]

SUPFAM

SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.

PROSITE

PS00597. PLANT_LTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P81402.

Entry information

Entry name

NLTP1_PRUPE

Accession

Primary (citable) accession number: P81402

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program