Reviewed, UniProtKB/Swiss-Prot P81380 (UCRI_RHOVI)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:	petA
Synonyms:	fbcF

Organism

Rhodopseudomonas viridis

Taxonomic identifier

1079 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Hyphomicrobiaceae › Blastochloris

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000127765
Regions
	Transmembrane	14 – 35	22	Potential
	Domain	80 – 173	94	Rieske
Sites
	Metal binding	118	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	120	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	137	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	140	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	123 ↔ 139		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81380-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: FEE328E1637DC49C	FASTA	179	19,011
	10 20 30 40 50 60 MASSDTAEAT RRDFLYVATA AVGAAGVAAV AWPFITQMNP DAATIAAGAP IDIDISPVTE 70 80 90 100 110 120 GQIVRVFWRG KPIFIRHRTA KEIQSEEAAD VGALIDPQPD SARVKPGKAE WLVVYASCTH 130 140 150 160 170 LGCIPLGHQG DWGGWFCPCH GSQYDASGRV RKGPAPTNLP VPPYEFVDNT KIRIGAGVA

References

[1]	"Cloning and sequencing of the fbcF, B and C genes encoding the cytochrome b/c1 complex from Rhodopseudomonas viridis." Verbist J., Lang F., Gabellini N., Oesterhelt D. Mol. Gen. Genet. 219:445-452(1989) [PubMed: 2560136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 133.

Cross-references

Sequence databases
PIR	JQ0345.
3D structure databases
HSSP	HSSP built from PDB template 1RIE based on UniProtKB P13272.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.2.2. 191710.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_RHOVI

Accession

Primary (citable) accession number: P81380

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 15, 1999
Last modified:	October 13, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)