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P81375

- OXLA_MACLB

UniProt

P81375 - OXLA_MACLB

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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Macrovipera lebetina (Levantine viper) (Vipera lebetina)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (highest specificity towards L-Met, L-Trp, L-Leu followed by L-His, L-Phe, L-Arg and L-Ile, wherease L-Gly, L-Ser, L-Thr, L-Pro, L-Cys, L-Asp are not oxidized), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as antibacterial activities against both Gram-negative (E.coli) and Gram-positive (B.subtilis) bacteria, and inhibition of agonist-induced platelet aggregation. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions. This protein may also induce hemorrhage, hemolysis, edema, apoptosis, and have antiparasitic activities.1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

Kineticsi

  1. KM=0.40 mM for L-Leu1 Publication
  2. KM=0.65 mM for L-Met1 Publication
  3. KM=0.17 mM for L-Trp1 Publication

Temperature dependencei

Thermostable between 4 and 25 degrees Celsius. At -20 degrees Celsius, the remaining activity is 20%. Heating at 70 degrees Celsius inactivates the enzyme in 15 minutes.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381SubstrateBy similarity
Binding sitei49 – 491SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 384FADBy similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. hemolysis in other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation inhibiting toxin, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
LAAO
Short name:
LAO
OrganismiMacrovipera lebetina (Levantine viper) (Vipera lebetina)
Taxonomic identifieri8709 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeMacrovipera

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›107›107L-amino-acid oxidasePRO_0000099872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi10 ↔ ?By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer; non-covalently linked.1 Publication

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Fragments.

P81375-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
ADDKNPLEEC FREDDYEEFL EIAKNGLEGW YANLGPMRYP VKPSEEGKHD
60 70 80 90 100
DIFAYEKFDE IVGGMDKKFW EDDGIHGGKE TFCYSPMIQK PYQFQHFSEA

LTAPVGR
Length:107
Mass (Da):12,435
Last modified:January 23, 2007 - v2
Checksum:i576D2F829779B412
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi27 – 282Curated
Non-adjacent residuesi38 – 392Curated
Non-adjacent residuesi48 – 492Curated
Non-adjacent residuesi57 – 582Curated
Non-adjacent residuesi67 – 682Curated
Non-adjacent residuesi79 – 802Curated
Non-adjacent residuesi90 – 912Curated
Non-terminal residuei107 – 1071

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. Tan C.H., Ang W.C.
    Submitted (MAY-1998) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-27.
    Tissue: Venom.
  2. "L-amino acid oxidase from Vipera lebetina venom: isolation, characterization, effects on platelets and bacteria."
    Tonismagi K., Samel M., Trummal K., Ronnholm G., Siigur J., Kalkkinen N., Siigur E.
    Toxicon 48:227-237(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-107, FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Venom.

Entry informationi

Entry nameiOXLA_MACLB
AccessioniPrimary (citable) accession number: P81375
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3