P81370 (TLP_ACTDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thaumatin-like protein

Alternative name(s):
Allergen=Act d 2

Gene names

Name:

tlp

Organism

Actinidia deliciosa (Kiwi)

Taxonomic identifier

3627 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Ericales › Actinidiaceae › Actinidia

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has antifungal activity By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Woody stem plug.
Post-translational modification	N-glycosylated. Ref.6
Allergenic properties	Causes an allergic reaction in human. Binds IgE. Ref.4 Ref.6
Sequence similarities	Belongs to the thaumatin family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Antimicrobial Fungicide Pathogenesis-related protein
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.3 Ref.4 Ref.5 Ref.6
	Chain	25 – 225	201	Thaumatin-like protein		PRO_0000096223
Amino acid modifications
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33 ↔ 224		By similarity
	Disulfide bond	74 ↔ 84		By similarity
	Disulfide bond	89 ↔ 95		By similarity
	Disulfide bond	140 ↔ 213		By similarity
	Disulfide bond	146 ↔ 196		By similarity
	Disulfide bond	154 ↔ 164		By similarity
	Disulfide bond	168 ↔ 177		By similarity
	Disulfide bond	178 ↔ 183		By similarity
Experimental info
	Sequence conflict	60	1	G → S in ABQ42566. Ref.2
	Sequence conflict	176	1	Y → F in ABQ42566. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P81370 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 229DABF722E7B961	FASTA	225	24,221
	10 20 30 40 50 60 MSTFKSLSLS ALLFIAFLFT CARGATFNII NNCPFTVWAA AVPGGGKRLD RGQNWIINPG 70 80 90 100 110 120 AGTKGARVWP RTGCNFDGAG RGKCQTGDCN GLLQCQAFGQ PPNTLAEYAL NQFNNLDFFD 130 140 150 160 170 180 ISLVDGFNVA MEFSPTSGGC TRGIKCTADI NGQCPNELRA PGGCNNPCTV FKTDQYCCNS 190 200 210 220 GNCGLTNFSK FFKDRCPDAY SYPKDDQTST FTCPAGTNYK VVFCP

References

[1]	Radauer C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fruit.
[2]	"Thaumatin-like kiwi protein, Act d 2 allergen." Gavrovic-Jankulovic M. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-225.
[3]	"Thaumatin-like protein in kiwifruit." Wurms K.V., Greenwood D.R., Sharrock K.R., Long P.G. J. Sci. Food Agric. 79:1448-1452(1999) [Agricola: IND22033965] Cited for: PROTEIN SEQUENCE OF 25-53. Strain: cv. Hayward. Tissue: Stem plug.
[4]	"IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries." Bublin M., Mari A., Ebner C., Knulst A., Scheiner O., Hoffmann-Sommergruber K., Breiteneder H., Radauer C. J. Allergy Clin. Immunol. 114:1169-1175(2004) [PubMed: 15536427] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-34, ALLERGEN. Strain: cv. Hayward. Tissue: Fruit.
[5]	"Kiwellin, a modular protein from green and gold kiwi fruits: evidence of in vivo and in vitro processing and IgE binding." Tuppo L., Giangrieco I., Palazzo P., Bernardi M.L., Scala E., Carratore V., Tamburrini M., Mari A., Ciardiello M.A. J. Agric. Food Chem. 56:3812-3817(2008) [PubMed: 18442249] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-32. Strain: cv. Hayward. Tissue: Fruit.
[6]	"Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms." Palacin A., Rodriguez J., Blanco C., Lopez-Torrejon G., Sanchez-Monge R., Varela J., Jimenez M.A., Cumplido J., Carrillo T., Crespo J.F., Salcedo G. Clin. Exp. Allergy 38:1220-1228(2008) [PubMed: 18205857] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-29, GLYCOSYLATION, ALLERGEN. Strain: cv. Hayward. Tissue: Fruit.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ871175 mRNA. Translation: CAI38795.2. EF417825 Genomic DNA. Translation: ABQ42566.1.
3D structure databases
ProteinModelPortal	P81370.
SMR	P81370. Positions 25-225.
ModBase	Search...
Protein family/group databases
Allergome	3053. Act d 2.0101. 747. Act d 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001938. Thaumatin. IPR017949. Thaumatin_CS. [Graphical view]
Gene3D	G3DSA:2.60.110.10. Thaumatin. 1 hit.
Pfam	PF00314. Thaumatin. 1 hit. [Graphical view]
PIRSF	PIRSF002703. Thaumatin. 1 hit.
PRINTS	PR00347. THAUMATIN.
SMART	SM00205. THN. 1 hit. [Graphical view]
SUPFAM	SSF49870. Thaumatin. 1 hit.
PROSITE	PS00316. THAUMATIN_1. 1 hit. PS51367. THAUMATIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TLP_ACTDE

Accession

Primary (citable) accession number: P81370
Secondary accession number(s): A5JGY0, Q5ND92

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 10, 2008
Last modified:	April 5, 2011
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program