Reviewed, UniProtKB/Swiss-Prot P81335 (VHUU_METJA)

Names and origin

Protein names

Recommended name:
    F420-non-reducing hydrogenase vhu subunit U
    EC=1.12.99.-

Gene names

Name:	vhuU
Ordered Locus Names:	MJ1192.1

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	50 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase vhu is composed of four subunits; vhuA, vhuD, vhuG and vhuU By similarity.
Miscellaneous	The large subunit of vhu is split into vhuA and vhuU. Each contributes two ligands to the [NiFeSe] center.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.
Sequence caution	The sequence AAB99197.1 differs from that shown. Reason: Erroneous termination at position 27. Translated as Sec.

Ontologies

Keywords
Coding sequence diversity	Selenocysteine
Ligand	Metal-binding Nickel Selenium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 33	33	F420-non-reducing hydrogenase vhu subunit U		PRO_0000013409
	Propeptide	34 – 50	17	Removed in mature form By similarity		PRO_0000013410
Sites
	Metal binding	27	1	Nickel Potential
	Metal binding	30	1	Nickel Potential
Amino acid modifications
	Non-standard residue	27	1	Selenocysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	P81335-1 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: A0BF6051D99602A0	FASTA	50	5,726
	10 20 30 40 50 MAEKSTVKVD EVKLNLIEMV LRAYDPUYSC AAHIIVKDEK GNKIIEVIKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion." Wilting R., Schorling S., Persson B.C., Boeck A. J. Mol. Biol. 266:637-641(1997) [PubMed: 9102456] [Abstract] Cited for: PROBABLE SELENOCYSTEINE AT SEC-27.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99197.1. Sequence problems.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus MJ1192.1 in contig L77117_GR.
TIGR	MJ1192.1.
Phylogenomic databases
HOGENOM	P81335.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1192.1-MON.
Family and domain databases
InterPro	IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
PROSITE	PS00508. NI_HGENASE_L_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VHUU_METJA

Accession

Primary (citable) accession number: P81335

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	February 26, 2008
Last modified:	May 5, 2009
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)