P81326 (T2M6_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type-2 restriction enzyme MjaVIP
Short name=R.MjaVIP
EC=3.1.21.4

Alternative name(s):
Endonuclease MjaVIP
Type II restriction enzyme MjaVIP

Gene names

Name:	mjaVIRP
Ordered Locus Names:	MJ1208.1

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded unmethylated sequence CCGG.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	To H.pylori HP0262.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Putative type-2 restriction enzyme MjaVIP		PRO_0000077337

Sequences

	Sequence		Length	Mass (Da)
	P81326 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9A7B306A28A6A2F2	FASTA	226	26,711
	10 20 30 40 50 60 MLHFGGFIME INHISKILEK EREEYIRNKV EEYLKQGFSK DDAVNKANQS WRTYIGHRIQ 70 80 90 100 110 120 DVIYNLLKKF LKDSGLKVTT DKALNNRNLP EELDKVKRLI AINYGEYLFL PDADVIVYKV 130 140 150 160 170 180 ENNDIKIIAI ISVKNSFRER RFETTYWKLK LKESPVTSHI KVFLATPDKD NEISYKCPNG 190 200 210 220 KPKKMRIILE YELDGIYFLK EDFEETEKAK HFGKIVEDII EISKKL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99221.1.
RefSeq	NP_248204.2. NC_000909.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	16564. MjaVIP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452612.
GenomeReviews	Gene locus MJ1208.1 in contig L77117_GR.
TIGR	MJ1208.1.
Phylogenomic databases
HOGENOM	HBG707743.
OMA	ERFTETP.
ProtClustDB	CLSK2792010.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1208.1-MONOMER.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2M6_METJA

Accession

Primary (citable) accession number: P81326

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 1, 1998
Last modified:	October 19, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program