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P81299

- CDC14_CAEEL

UniProt

P81299 - CDC14_CAEEL

Protein

Probable tyrosine-protein phosphatase cdc-14

Gene

cdc-14

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    Has a role in cell division: functions upstream of the cki-1 Cip/Kip inhibitor to promote accumulation of cki-1 in the nucleus (a hypophosphorylated and stable form of cki-1 is required for developmentally programmed cell-cycle arrest). Isoform c is a regulator of central spindle formation and cytokinesis. Co-localizes with zen-4.2 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.By similarityPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei295 – 2951Phosphocysteine intermediatePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri950 – 102475THAP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. phosphatase activity Source: WormBase
    4. phosphoprotein phosphatase activity Source: WormBase
    5. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
    6. protein tyrosine phosphatase activity Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: WormBase
    2. microtubule cytoskeleton organization Source: UniProtKB
    3. mitotic cell cycle arrest Source: WormBase
    4. mitotic cytokinesis Source: WormBase
    5. negative regulation of cell cycle Source: UniProtKB
    6. peptidyl-tyrosine dephosphorylation Source: GOC
    7. polar body extrusion after meiotic divisions Source: WormBase
    8. protein dephosphorylation Source: WormBase
    9. regulation of cell development Source: UniProtKB
    10. regulation of exit from mitosis Source: InterPro
    11. spindle midzone assembly involved in mitosis Source: WormBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tyrosine-protein phosphatase cdc-14 (EC:3.1.3.48)
    Alternative name(s):
    Cell division cycle-related protein 14
    Gene namesi
    Name:cdc-14
    ORF Names:C17G10.4
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome II

    Organism-specific databases

    WormBaseiC17G10.4a; CE30868; WBGene00000383; cdc-14.
    C17G10.4b; CE08287; WBGene00000383; cdc-14.
    C17G10.4c; CE08288; WBGene00000383; cdc-14.
    C17G10.4d; CE06845; WBGene00000383; cdc-14.
    C17G10.4e; CE36916; WBGene00000383; cdc-14.

    Subcellular locationi

    Isoform c : Cytoplasmcytoskeletonspindle 1 Publication
    Note: Localizes to the central spindle in anaphase and to the midbody in telophase. Localizes dynamically and accumulates in the cytoplasm during G1 phase.

    GO - Cellular componenti

    1. astral microtubule Source: WormBase
    2. centrosome Source: WormBase
    3. cytoplasm Source: UniProtKB
    4. midbody Source: WormBase
    5. mitotic spindle midzone Source: WormBase
    6. nucleolus Source: WormBase
    7. nucleus Source: WormBase
    8. spindle Source: UniProtKB
    9. spindle midzone Source: WormBase

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    Pathology & Biotechi

    Disruption phenotypei

    Worms produce embryos that fail to establish a central spindle and exhibit several mis-localized proteins. This results in extra divisions in multiple lineages, with no apparent defects in mitosis or cell-fate determination.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi295 – 2951C → S: Abolishes phosphatase activity (in isoform c). 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10631063Probable tyrosine-protein phosphatase cdc-14PRO_0000094880Add
    BLAST

    Proteomic databases

    PaxDbiP81299.
    PRIDEiP81299.

    Interactioni

    Protein-protein interaction databases

    BioGridi39287. 4 interactions.
    DIPiDIP-25903N.
    MINTiMINT-1063538.

    Structurei

    3D structure databases

    ProteinModelPortaliP81299.
    SMRiP81299. Positions 41-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini278 – 34063Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 THAP-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri950 – 102475THAP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG2453.
    GeneTreeiENSGT00390000010254.
    InParanoidiP81299.
    KOiK06639.
    OMAiHYSEAST.

    Family and domain databases

    Gene3Di3.90.190.10. 2 hits.
    InterProiIPR026070. CDC14.
    IPR029260. DSPn.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR006612. Znf_C2CH.
    [Graphical view]
    PANTHERiPTHR23339:SF27. PTHR23339:SF27. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF14671. DSPn. 1 hit.
    PF05485. THAP. 1 hit.
    [Graphical view]
    SMARTiSM00692. DM3. 1 hit.
    SM00980. THAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 2 hits.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50950. ZF_THAP. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform bCurated (identifier: P81299-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN     50
    INNKFHYEPF YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD 100
    GTGQYDKIRV NTAYVLGAYL IIYQGFSADD AYLKVSSGET VKFVGFRDAS 150
    MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD FDYEEYEFYE RVENGDFNWI 200
    IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST IVRLNAKNYD 250
    ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG 300
    RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS 350
    LSQSNGVHLT QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR 400
    RRVQVQNGRS TAPVTIAPAG TSESRRSTKP SRVVDETALD DQGRSQGDRL 450
    LQLKAKHQHE SETTSPNSSS SRRFVKSSTP QMTVPSQAYL NRNREPIIVT 500
    PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR TPASAVFPSM 550
    ASRRSETTRY LSPTTPIKPM SPSYTDGTSP RYKARLRSEN PIGSTTSTPF 600
    SLQPQFGLVR VPPDSPHSIM AHRPPPTTSS RAPLSPHNYS TTQGYSTSSR 650
    GLYGDKKPLA RGSVSTSTLP SMYMTRKMSE NGAVEVEITT SYVEDRKAAE 700
    QIDPSSTPPE PKRRFVLVSR KIVSKEVPSA GPTCSNDPRL NYASTRKLPI 750
    KISHRLCQIC RTIKPIADIS MMTDENEKLF ILLAAIFQRK LFVEECTRIY 800
    KQPAVYTCSS HYSEASTAIL NMFGVGGALR IFRARPRKVS QVEKFASHLM 850
    GKQFEEDKLI HSAYMFVLNH PEAGAVTGPI IKEEDIPFEE TVECSSTTAV 900
    NGILPKCFRQ PRKQKLEEDS CSSSILRIIK KTTPSLPNRS YTMINQNMEH 950
    PLQCCYCLEV YEKRYMTQVP KTEQRIARWV AILGEQFRIR LRMKPANYMC 1000
    RKHFPQADFS SRGRLLKTAV PNVVSQEKVL AFKLEGNTFV EVKEDNTNIK 1050
    MEKGTDSEID IEN 1063
    Length:1,063
    Mass (Da):120,249
    Last modified:April 30, 2003 - v2
    Checksum:i2B0494459F710197
    GO
    Isoform aCurated (identifier: P81299-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS
         694-1063: Missing.

    Show »
    Length:693
    Mass (Da):77,865
    Checksum:i9013D05AD9FB560D
    GO
    Isoform cCurated (identifier: P81299-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         677-681: KMSEN → SCERK
         682-1063: Missing.

    Show »
    Length:681
    Mass (Da):76,698
    Checksum:i2C056CD6FA03ADDC
    GO
    Isoform dCurated (identifier: P81299-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         606-695: FGLVRVPPDS...VEITTSYVED → ITKCSLTAES...GVCKLVNTLS
         696-1063: Missing.

    Show »
    Length:695
    Mass (Da):77,995
    Checksum:iE834240A433EDED1
    GO
    Isoform e (identifier: P81299-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         541-541: T → TGNESYRFHNSLFYE
         606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS
         694-1063: Missing.

    Show »
    Length:707
    Mass (Da):79,610
    Checksum:iE7C1549ADC2625C3
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei541 – 5411T → TGNESYRFHNSLFYE in isoform e. 1 PublicationVSP_021641
    Alternative sequencei606 – 69590FGLVR…SYVED → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GVCKLVNTLS in isoform d. 1 PublicationVSP_050492Add
    BLAST
    Alternative sequencei606 – 69388FGLVR…TTSYV → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GKRRKTAS in isoform a and isoform e. 1 PublicationVSP_050491Add
    BLAST
    Alternative sequencei677 – 6815KMSEN → SCERK in isoform c. 2 PublicationsVSP_050493
    Alternative sequencei682 – 1063382Missing in isoform c. 2 PublicationsVSP_050494Add
    BLAST
    Alternative sequencei694 – 1063370Missing in isoform a and isoform e. 1 PublicationVSP_050495Add
    BLAST
    Alternative sequencei696 – 1063368Missing in isoform d. 1 PublicationVSP_050496Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY661743 mRNA. Translation: AAT74542.1.
    AY661744 mRNA. Translation: AAT74543.1.
    AY661745 mRNA. Translation: AAT74544.1.
    AY661746 mRNA. Translation: AAT74545.1.
    AY661747 mRNA. Translation: AAT74546.1.
    AF000363 mRNA. Translation: AAB94407.1.
    FO080593 Genomic DNA. Translation: CCD64951.1.
    FO080593 Genomic DNA. Translation: CCD64952.1.
    FO080593 Genomic DNA. Translation: CCD64953.1.
    FO080593 Genomic DNA. Translation: CCD64954.1.
    FO080593 Genomic DNA. Translation: CCD64955.1.
    PIRiE88158.
    T34097.
    RefSeqiNP_001021969.1. NM_001026798.5. [P81299-5]
    NP_495084.3. NM_062683.7. [P81299-1]
    NP_495085.2. NM_062684.5. [P81299-2]
    NP_495086.1. NM_062685.3. [P81299-3]
    NP_740991.1. NM_170993.4. [P81299-4]
    UniGeneiCel.17299.

    Genome annotation databases

    EnsemblMetazoaiC17G10.4b.1; C17G10.4b.1; WBGene00000383. [P81299-1]
    C17G10.4b.2; C17G10.4b.2; WBGene00000383. [P81299-1]
    GeneIDi173945.
    KEGGicel:CELE_C17G10.4.
    UCSCiC17G10.4b. c. elegans. [P81299-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY661743 mRNA. Translation: AAT74542.1 .
    AY661744 mRNA. Translation: AAT74543.1 .
    AY661745 mRNA. Translation: AAT74544.1 .
    AY661746 mRNA. Translation: AAT74545.1 .
    AY661747 mRNA. Translation: AAT74546.1 .
    AF000363 mRNA. Translation: AAB94407.1 .
    FO080593 Genomic DNA. Translation: CCD64951.1 .
    FO080593 Genomic DNA. Translation: CCD64952.1 .
    FO080593 Genomic DNA. Translation: CCD64953.1 .
    FO080593 Genomic DNA. Translation: CCD64954.1 .
    FO080593 Genomic DNA. Translation: CCD64955.1 .
    PIRi E88158.
    T34097.
    RefSeqi NP_001021969.1. NM_001026798.5. [P81299-5 ]
    NP_495084.3. NM_062683.7. [P81299-1 ]
    NP_495085.2. NM_062684.5. [P81299-2 ]
    NP_495086.1. NM_062685.3. [P81299-3 ]
    NP_740991.1. NM_170993.4. [P81299-4 ]
    UniGenei Cel.17299.

    3D structure databases

    ProteinModelPortali P81299.
    SMRi P81299. Positions 41-349.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 39287. 4 interactions.
    DIPi DIP-25903N.
    MINTi MINT-1063538.

    Proteomic databases

    PaxDbi P81299.
    PRIDEi P81299.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C17G10.4b.1 ; C17G10.4b.1 ; WBGene00000383 . [P81299-1 ]
    C17G10.4b.2 ; C17G10.4b.2 ; WBGene00000383 . [P81299-1 ]
    GeneIDi 173945.
    KEGGi cel:CELE_C17G10.4.
    UCSCi C17G10.4b. c. elegans. [P81299-1 ]

    Organism-specific databases

    CTDi 173945.
    WormBasei C17G10.4a ; CE30868 ; WBGene00000383 ; cdc-14.
    C17G10.4b ; CE08287 ; WBGene00000383 ; cdc-14.
    C17G10.4c ; CE08288 ; WBGene00000383 ; cdc-14.
    C17G10.4d ; CE06845 ; WBGene00000383 ; cdc-14.
    C17G10.4e ; CE36916 ; WBGene00000383 ; cdc-14.

    Phylogenomic databases

    eggNOGi COG2453.
    GeneTreei ENSGT00390000010254.
    InParanoidi P81299.
    KOi K06639.
    OMAi HYSEAST.

    Miscellaneous databases

    NextBioi 881775.
    PROi P81299.

    Family and domain databases

    Gene3Di 3.90.190.10. 2 hits.
    InterProi IPR026070. CDC14.
    IPR029260. DSPn.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR006612. Znf_C2CH.
    [Graphical view ]
    PANTHERi PTHR23339:SF27. PTHR23339:SF27. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF14671. DSPn. 1 hit.
    PF05485. THAP. 1 hit.
    [Graphical view ]
    SMARTi SM00692. DM3. 1 hit.
    SM00980. THAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 2 hits.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50950. ZF_THAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans."
      Saito R.M., Perreault A., Peach B., Satterlee J.S., van den Heuvel S.
      Nat. Cell Biol. 6:777-783(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E), FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    2. Ernsting B.R., Li L., Wishart M.J., Dixon J.E.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    4. "The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo."
      Gruneberg U., Glotzer M., Gartner A., Nigg E.A.
      J. Cell Biol. 158:901-914(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION (ISOFORM C), MUTAGENESIS OF CYS-295.

    Entry informationi

    Entry nameiCDC14_CAEEL
    AccessioniPrimary (citable) accession number: P81299
    Secondary accession number(s): P81300
    , Q09955, Q09976, Q6DLY2, Q6DLY3, Q6DLY4, Q6DLY5, Q6DLY6, Q8MQD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3