Probable tyrosine-protein phosphatase cdc-14

Names and origin

Protein names

Recommended name:
    Probable tyrosine-protein phosphatase cdc-14
    EC=3.1.3.48
Alternative name(s):
    Cell division cycle-related protein 14

Gene names

Name:	cdc-14
ORF Names:	C17G10.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	1063 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Has a role in cell division: functions upstream of the cki-1 Cip/Kip inhibitor to promote accumulation of cki-1 in the nucleus (a hypophosphorylated and stable form of cki-1 is required for developmentally programmed cell-cycle arrest). Isoform c is a regulator of central spindle formation and cytokinesis. Co-localizes with zen-4. Ref.1 Ref.4
Catalytic activity	Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate. UniProtKB Q00684
Subcellular location	Isoform c: Cytoplasm › cytoskeleton › spindle. Note: Localizes to the central spindle in anaphase and to the midbody in telophase. Localizes dynamically and accumulates in the cytoplasm during G1 phase. Ref.1 Ref.4
Disruption phenotype	Worms produce embryos that fail to establish a central spindle and exhibit several mis-localized proteins. This results in extra divisions in multiple lineages, with no apparent defects in mitosis or cell-fate determination. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. Contains 1 THAP-type zinc finger. Contains 1 tyrosine-protein phosphatase domain.

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Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cellular response to DNA damage stimulus Inferred from mutant phenotype. Source: WormBase cytokinesis Ref.4 Inferred from mutant phenotype. Source: UniProtKB embryonic development ending in birth or egg hatching Ref.4 Inferred from mutant phenotype. Source: WormBase negative regulation of cell cycle Ref.1 Inferred from direct assay. Source: UniProtKB positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro regulation of cell development Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB microtubule Inferred from electronic annotation. Source: UniProtKB-KW spindle Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Ref.1 Ref.4 Traceable author statement. Source: UniProtKB protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform b (identifier: P81299-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform a (identifier: P81299-2) The sequence of this isoform differs from the canonical sequence as follows: 606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS 694-1063: Missing.

Isoform c (identifier: P81299-3) The sequence of this isoform differs from the canonical sequence as follows: 677-681: KMSEN → SCERK 682-1063: Missing.
Note: Localizes to the central spindle in anaphase and to the midbody in telophase. Localizes dynamically and accumulates in the cytoplasm during G1 phase. Ref.1 Ref.4

Isoform d (identifier: P81299-4) The sequence of this isoform differs from the canonical sequence as follows: 606-695: FGLVRVPPDS...VEITTSYVED → ITKCSLTAES...GVCKLVNTLS 696-1063: Missing.

Isoform e (identifier: P81299-5) The sequence of this isoform differs from the canonical sequence as follows: 541-541: T → TGNESYRFHNSLFYE 606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS 694-1063: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1063

1063

Probable tyrosine-protein phosphatase cdc-14

PRO_0000094880

Regions

Domain

278 – 340

63

Tyrosine-protein phosphatase

Zinc finger

950 – 1024

75

THAP-type

Sites

Active site

295

1

Phosphocysteine intermediate By similarity UniProtKB Q00684

Natural variations

Alternative sequence

541

1

T → TGNESYRFHNSLFYE in isoform e.

VSP_021641

Alternative sequence

606 – 695

90

FGLVR…SYVED → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GVCKLVNTLS in isoform d.

VSP_050492

Alternative sequence

606 – 693

88

FGLVR…TTSYV → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GKRRKTAS in isoform a and isoform e.

VSP_050491

Alternative sequence

677 – 681

5

KMSEN → SCERK in isoform c.

VSP_050493

Alternative sequence

682 – 1063

382

Missing in isoform c.

VSP_050494

Alternative sequence

694 – 1063

370

Missing in isoform a and isoform e.

VSP_050495

Alternative sequence

696 – 1063

368

Missing in isoform d.

VSP_050496

Experimental info

Mutagenesis

295

1

C → S: Abolishes phosphatase activity (in isoform c). Ref.4

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Sequences

Sequence		Length	Mass (Da)
Isoform b [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 2B0494459F710197 Show »	FASTA	1,063	120,249
10 20 30 40 50 60 MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN INNKFHYEPF 70 80 90 100 110 120 YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD GTGQYDKIRV NTAYVLGAYL 130 140 150 160 170 180 IIYQGFSADD AYLKVSSGET VKFVGFRDAS MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD 190 200 210 220 230 240 FDYEEYEFYE RVENGDFNWI IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST 250 260 270 280 290 300 IVRLNAKNYD ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG 310 320 330 340 350 360 RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS LSQSNGVHLT 370 380 390 400 410 420 QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR RRVQVQNGRS TAPVTIAPAG 430 440 450 460 470 480 TSESRRSTKP SRVVDETALD DQGRSQGDRL LQLKAKHQHE SETTSPNSSS SRRFVKSSTP 490 500 510 520 530 540 QMTVPSQAYL NRNREPIIVT PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR 550 560 570 580 590 600 TPASAVFPSM ASRRSETTRY LSPTTPIKPM SPSYTDGTSP RYKARLRSEN PIGSTTSTPF 610 620 630 640 650 660 SLQPQFGLVR VPPDSPHSIM AHRPPPTTSS RAPLSPHNYS TTQGYSTSSR GLYGDKKPLA 670 680 690 700 710 720 RGSVSTSTLP SMYMTRKMSE NGAVEVEITT SYVEDRKAAE QIDPSSTPPE PKRRFVLVSR 730 740 750 760 770 780 KIVSKEVPSA GPTCSNDPRL NYASTRKLPI KISHRLCQIC RTIKPIADIS MMTDENEKLF 790 800 810 820 830 840 ILLAAIFQRK LFVEECTRIY KQPAVYTCSS HYSEASTAIL NMFGVGGALR IFRARPRKVS 850 860 870 880 890 900 QVEKFASHLM GKQFEEDKLI HSAYMFVLNH PEAGAVTGPI IKEEDIPFEE TVECSSTTAV 910 920 930 940 950 960 NGILPKCFRQ PRKQKLEEDS CSSSILRIIK KTTPSLPNRS YTMINQNMEH PLQCCYCLEV 970 980 990 1000 1010 1020 YEKRYMTQVP KTEQRIARWV AILGEQFRIR LRMKPANYMC RKHFPQADFS SRGRLLKTAV 1030 1040 1050 1060 PNVVSQEKVL AFKLEGNTFV EVKEDNTNIK MEKGTDSEID IEN « Hide
Isoform a. Checksum: 9013D05AD9FB560D Show »	FASTA	693	77,865
10 20 30 40 50 60 MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN INNKFHYEPF 70 80 90 100 110 120 YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD GTGQYDKIRV NTAYVLGAYL 130 140 150 160 170 180 IIYQGFSADD AYLKVSSGET VKFVGFRDAS MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD 190 200 210 220 230 240 FDYEEYEFYE RVENGDFNWI IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST 250 260 270 280 290 300 IVRLNAKNYD ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG 310 320 330 340 350 360 RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS LSQSNGVHLT 370 380 390 400 410 420 QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR RRVQVQNGRS TAPVTIAPAG 430 440 450 460 470 480 TSESRRSTKP SRVVDETALD DQGRSQGDRL LQLKAKHQHE SETTSPNSSS SRRFVKSSTP 490 500 510 520 530 540 QMTVPSQAYL NRNREPIIVT PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR 550 560 570 580 590 600 TPASAVFPSM ASRRSETTRY LSPTTPIKPM SPSYTDGTSP RYKARLRSEN PIGSTTSTPF 610 620 630 640 650 660 SLQPQITKCS LTAESKPPKR ILSMPGTSKS TSSLKKIQVS RPRPYPSTGV RVELCANGKS 670 680 690 YDIRPRKEAH VIPGAGLAAN TEALLGKRRK TAS « Hide
Isoform c. Checksum: 2C056CD6FA03ADDC Show »	FASTA	681	76,698
10 20 30 40 50 60 MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN INNKFHYEPF 70 80 90 100 110 120 YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD GTGQYDKIRV NTAYVLGAYL 130 140 150 160 170 180 IIYQGFSADD AYLKVSSGET VKFVGFRDAS MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD 190 200 210 220 230 240 FDYEEYEFYE RVENGDFNWI IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST 250 260 270 280 290 300 IVRLNAKNYD ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG 310 320 330 340 350 360 RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS LSQSNGVHLT 370 380 390 400 410 420 QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR RRVQVQNGRS TAPVTIAPAG 430 440 450 460 470 480 TSESRRSTKP SRVVDETALD DQGRSQGDRL LQLKAKHQHE SETTSPNSSS SRRFVKSSTP 490 500 510 520 530 540 QMTVPSQAYL NRNREPIIVT PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR 550 560 570 580 590 600 TPASAVFPSM ASRRSETTRY LSPTTPIKPM SPSYTDGTSP RYKARLRSEN PIGSTTSTPF 610 620 630 640 650 660 SLQPQFGLVR VPPDSPHSIM AHRPPPTTSS RAPLSPHNYS TTQGYSTSSR GLYGDKKPLA 670 680 RGSVSTSTLP SMYMTRSCER K « Hide
Isoform d. Checksum: E834240A433EDED1 Show »	FASTA	695	77,995
10 20 30 40 50 60 MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN INNKFHYEPF 70 80 90 100 110 120 YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD GTGQYDKIRV NTAYVLGAYL 130 140 150 160 170 180 IIYQGFSADD AYLKVSSGET VKFVGFRDAS MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD 190 200 210 220 230 240 FDYEEYEFYE RVENGDFNWI IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST 250 260 270 280 290 300 IVRLNAKNYD ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG 310 320 330 340 350 360 RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS LSQSNGVHLT 370 380 390 400 410 420 QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR RRVQVQNGRS TAPVTIAPAG 430 440 450 460 470 480 TSESRRSTKP SRVVDETALD DQGRSQGDRL LQLKAKHQHE SETTSPNSSS SRRFVKSSTP 490 500 510 520 530 540 QMTVPSQAYL NRNREPIIVT PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR 550 560 570 580 590 600 TPASAVFPSM ASRRSETTRY LSPTTPIKPM SPSYTDGTSP RYKARLRSEN PIGSTTSTPF 610 620 630 640 650 660 SLQPQITKCS LTAESKPPKR ILSMPGTSKS TSSLKKIQVS RPRPYPSTGV RVELCANGKS 670 680 690 YDIRPRKEAH VIPGAGLAAN TEALLGVCKL VNTLS « Hide
Isoform e. Checksum: E7C1549ADC2625C3 Show »	FASTA	707	79,610
10 20 30 40 50 60 MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN INNKFHYEPF 70 80 90 100 110 120 YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD GTGQYDKIRV NTAYVLGAYL 130 140 150 160 170 180 IIYQGFSADD AYLKVSSGET VKFVGFRDAS MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD 190 200 210 220 230 240 FDYEEYEFYE RVENGDFNWI IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST 250 260 270 280 290 300 IVRLNAKNYD ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG 310 320 330 340 350 360 RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS LSQSNGVHLT 370 380 390 400 410 420 QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR RRVQVQNGRS TAPVTIAPAG 430 440 450 460 470 480 TSESRRSTKP SRVVDETALD DQGRSQGDRL LQLKAKHQHE SETTSPNSSS SRRFVKSSTP 490 500 510 520 530 540 QMTVPSQAYL NRNREPIIVT PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR 550 560 570 580 590 600 TGNESYRFHN SLFYEPASAV FPSMASRRSE TTRYLSPTTP IKPMSPSYTD GTSPRYKARL 610 620 630 640 650 660 RSENPIGSTT STPFSLQPQI TKCSLTAESK PPKRILSMPG TSKSTSSLKK IQVSRPRPYP 670 680 690 700 STGVRVELCA NGKSYDIRPR KEAHVIPGAG LAANTEALLG KRRKTAS « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans." Saito R.M., Perreault A., Peach B., Satterlee J.S., van den Heuvel S. Nat. Cell Biol. 6:777-783(2004) [PubMed: 15247923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E), FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]	Ernsting B.R., Li L., Wishart M.J., Dixon J.E. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[4]	"The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo." Gruneberg U., Glotzer M., Gartner A., Nigg E.A. J. Cell Biol. 158:901-914(2002) [PubMed: 12213836] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION OF ISOFORM C, MUTAGENESIS OF CYS-295.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AY661743 mRNA. Translation: AAT74542.1. AY661744 mRNA. Translation: AAT74543.1. AY661745 mRNA. Translation: AAT74544.1. AY661746 mRNA. Translation: AAT74545.1. AY661747 mRNA. Translation: AAT74546.1. AF000363 mRNA. Translation: AAB94407.1. U28739 Genomic DNA. Translation: AAB93458.1. U28739 Genomic DNA. Translation: AAB93459.1. U28739 Genomic DNA. Translation: AAM54205.1. U28739 Genomic DNA. Translation: AAM54206.1. U28739 Genomic DNA. Translation: AAT68903.1.
PIR	E88158. T34097.
RefSeq	NP_001021969.1. NP_495084.3. NP_495085.2. NP_495086.1. NP_740991.1.
UniGene	Cel.17299
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25903N.
IntAct	P81299. 4 interactions.
STRING	P81299.
Proteomic databases
PRIDE	P81299.
Genome annotation databases
Ensembl	C17G10.4b; C17G10.4b; C17G10.4; Caenorhabditis elegans. [Genome view]
GeneID	173945.
KEGG	cel:C17G10.4.
NMPDR	fig\|6239.3.peg.5589.
UCSC	C17G10.4b. c. elegans.
Organism-specific databases
CTD	173945.
WormBase	WBGene00000383. cdc-14.
WormPep	C17G10.4a. CE30868. [WorfDB] C17G10.4b. CE08287. [WorfDB] C17G10.4c. CE08288. [WorfDB] C17G10.4d. CE06845. [WorfDB] C17G10.4e. CE36916. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04180.
InParanoid	P81299.
OMA	CANGKSY.
PhylomeDB	P81299.
Enzyme and pathway databases
BRENDA	3.1.3.48. 672.
Gene expression databases
ArrayExpress	P81299.
Family and domain databases
InterPro	IPR000387. Dual-sp/Tyr_phosphatase. IPR000340. Dual-sp_phosphatase_cat-dom. IPR016130. Tyr_Pase_AS. IPR006612. Znf_C2CH. [Graphical view]
Pfam	PF00782. DSPc. 1 hit. PF05485. THAP. 1 hit. [Graphical view]
SMART	SM00692. DM3. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. False negative. PS50950. ZF_THAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	881775.

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Entry information

Entry name

CDC14_CAEEL

Accession

Primary (citable) accession number: P81299
Secondary accession number(s): P81300

Q8MQD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	February 9, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents