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P81299

- CDC14_CAEEL

UniProt

P81299 - CDC14_CAEEL

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Protein

Probable tyrosine-protein phosphatase cdc-14

Gene

cdc-14

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a role in cell division: functions upstream of the cki-1 Cip/Kip inhibitor to promote accumulation of cki-1 in the nucleus (a hypophosphorylated and stable form of cki-1 is required for developmentally programmed cell-cycle arrest). Isoform c is a regulator of central spindle formation and cytokinesis. Co-localizes with zen-4.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.By similarityPROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei295 – 2951Phosphocysteine intermediatePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri950 – 102475THAP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. phosphatase activity Source: WormBase
  4. phosphoprotein phosphatase activity Source: WormBase
  5. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  6. protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: WormBase
  2. microtubule cytoskeleton organization Source: UniProtKB
  3. mitotic cell cycle arrest Source: WormBase
  4. mitotic cytokinesis Source: WormBase
  5. negative regulation of cell cycle Source: UniProtKB
  6. peptidyl-tyrosine dephosphorylation Source: GOC
  7. polar body extrusion after meiotic divisions Source: WormBase
  8. protein dephosphorylation Source: WormBase
  9. regulation of cell development Source: UniProtKB
  10. regulation of exit from mitosis Source: InterPro
  11. spindle midzone assembly involved in mitosis Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tyrosine-protein phosphatase cdc-14 (EC:3.1.3.48)
Alternative name(s):
Cell division cycle-related protein 14
Gene namesi
Name:cdc-14
ORF Names:C17G10.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiC17G10.4a; CE30868; WBGene00000383; cdc-14.
C17G10.4b; CE08287; WBGene00000383; cdc-14.
C17G10.4c; CE08288; WBGene00000383; cdc-14.
C17G10.4d; CE06845; WBGene00000383; cdc-14.
C17G10.4e; CE36916; WBGene00000383; cdc-14.

Subcellular locationi

Isoform c : Cytoplasmcytoskeletonspindle 1 Publication
Note: Localizes to the central spindle in anaphase and to the midbody in telophase. Localizes dynamically and accumulates in the cytoplasm during G1 phase.

GO - Cellular componenti

  1. astral microtubule Source: WormBase
  2. centrosome Source: WormBase
  3. cytoplasm Source: UniProtKB
  4. midbody Source: WormBase
  5. mitotic spindle midzone Source: WormBase
  6. nucleolus Source: WormBase
  7. nucleus Source: WormBase
  8. spindle Source: UniProtKB
  9. spindle midzone Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Worms produce embryos that fail to establish a central spindle and exhibit several mis-localized proteins. This results in extra divisions in multiple lineages, with no apparent defects in mitosis or cell-fate determination.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi295 – 2951C → S: Abolishes phosphatase activity (in isoform c). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10631063Probable tyrosine-protein phosphatase cdc-14PRO_0000094880Add
BLAST

Proteomic databases

PaxDbiP81299.
PRIDEiP81299.

Interactioni

Protein-protein interaction databases

BioGridi39287. 4 interactions.
DIPiDIP-25903N.
MINTiMINT-1063538.

Structurei

3D structure databases

ProteinModelPortaliP81299.
SMRiP81299. Positions 41-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini278 – 34063Tyrosine-protein phosphataseAdd
BLAST

Sequence similaritiesi

Contains 1 THAP-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri950 – 102475THAP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00390000010254.
InParanoidiP81299.
KOiK06639.
OMAiHYSEAST.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiIPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR006612. Znf_C2CH.
[Graphical view]
PANTHERiPTHR23339:SF27. PTHR23339:SF27. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
PF05485. THAP. 1 hit.
[Graphical view]
SMARTiSM00692. DM3. 1 hit.
SM00980. THAP. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50950. ZF_THAP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform bCurated (identifier: P81299) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN
60 70 80 90 100
INNKFHYEPF YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD
110 120 130 140 150
GTGQYDKIRV NTAYVLGAYL IIYQGFSADD AYLKVSSGET VKFVGFRDAS
160 170 180 190 200
MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD FDYEEYEFYE RVENGDFNWI
210 220 230 240 250
IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST IVRLNAKNYD
260 270 280 290 300
ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG
310 320 330 340 350
RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS
360 370 380 390 400
LSQSNGVHLT QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR
410 420 430 440 450
RRVQVQNGRS TAPVTIAPAG TSESRRSTKP SRVVDETALD DQGRSQGDRL
460 470 480 490 500
LQLKAKHQHE SETTSPNSSS SRRFVKSSTP QMTVPSQAYL NRNREPIIVT
510 520 530 540 550
PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR TPASAVFPSM
560 570 580 590 600
ASRRSETTRY LSPTTPIKPM SPSYTDGTSP RYKARLRSEN PIGSTTSTPF
610 620 630 640 650
SLQPQFGLVR VPPDSPHSIM AHRPPPTTSS RAPLSPHNYS TTQGYSTSSR
660 670 680 690 700
GLYGDKKPLA RGSVSTSTLP SMYMTRKMSE NGAVEVEITT SYVEDRKAAE
710 720 730 740 750
QIDPSSTPPE PKRRFVLVSR KIVSKEVPSA GPTCSNDPRL NYASTRKLPI
760 770 780 790 800
KISHRLCQIC RTIKPIADIS MMTDENEKLF ILLAAIFQRK LFVEECTRIY
810 820 830 840 850
KQPAVYTCSS HYSEASTAIL NMFGVGGALR IFRARPRKVS QVEKFASHLM
860 870 880 890 900
GKQFEEDKLI HSAYMFVLNH PEAGAVTGPI IKEEDIPFEE TVECSSTTAV
910 920 930 940 950
NGILPKCFRQ PRKQKLEEDS CSSSILRIIK KTTPSLPNRS YTMINQNMEH
960 970 980 990 1000
PLQCCYCLEV YEKRYMTQVP KTEQRIARWV AILGEQFRIR LRMKPANYMC
1010 1020 1030 1040 1050
RKHFPQADFS SRGRLLKTAV PNVVSQEKVL AFKLEGNTFV EVKEDNTNIK
1060
MEKGTDSEID IEN
Length:1,063
Mass (Da):120,249
Last modified:April 30, 2003 - v2
Checksum:i2B0494459F710197
GO
Isoform aCurated (identifier: P81299-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS
     694-1063: Missing.

Show »
Length:693
Mass (Da):77,865
Checksum:i9013D05AD9FB560D
GO
Isoform cCurated (identifier: P81299-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     677-681: KMSEN → SCERK
     682-1063: Missing.

Show »
Length:681
Mass (Da):76,698
Checksum:i2C056CD6FA03ADDC
GO
Isoform dCurated (identifier: P81299-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-695: FGLVRVPPDS...VEITTSYVED → ITKCSLTAES...GVCKLVNTLS
     696-1063: Missing.

Show »
Length:695
Mass (Da):77,995
Checksum:iE834240A433EDED1
GO
Isoform e (identifier: P81299-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     541-541: T → TGNESYRFHNSLFYE
     606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS
     694-1063: Missing.

Show »
Length:707
Mass (Da):79,610
Checksum:iE7C1549ADC2625C3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei541 – 5411T → TGNESYRFHNSLFYE in isoform e. 1 PublicationVSP_021641
Alternative sequencei606 – 69590FGLVR…SYVED → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GVCKLVNTLS in isoform d. 1 PublicationVSP_050492Add
BLAST
Alternative sequencei606 – 69388FGLVR…TTSYV → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GKRRKTAS in isoform a and isoform e. 1 PublicationVSP_050491Add
BLAST
Alternative sequencei677 – 6815KMSEN → SCERK in isoform c. 2 PublicationsVSP_050493
Alternative sequencei682 – 1063382Missing in isoform c. 2 PublicationsVSP_050494Add
BLAST
Alternative sequencei694 – 1063370Missing in isoform a and isoform e. 1 PublicationVSP_050495Add
BLAST
Alternative sequencei696 – 1063368Missing in isoform d. 1 PublicationVSP_050496Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY661743 mRNA. Translation: AAT74542.1.
AY661744 mRNA. Translation: AAT74543.1.
AY661745 mRNA. Translation: AAT74544.1.
AY661746 mRNA. Translation: AAT74545.1.
AY661747 mRNA. Translation: AAT74546.1.
AF000363 mRNA. Translation: AAB94407.1.
FO080593 Genomic DNA. Translation: CCD64951.1.
FO080593 Genomic DNA. Translation: CCD64952.1.
FO080593 Genomic DNA. Translation: CCD64953.1.
FO080593 Genomic DNA. Translation: CCD64954.1.
FO080593 Genomic DNA. Translation: CCD64955.1.
PIRiE88158.
T34097.
RefSeqiNP_001021969.1. NM_001026798.5. [P81299-5]
NP_495084.3. NM_062683.7. [P81299-1]
NP_495085.2. NM_062684.5. [P81299-2]
NP_495086.1. NM_062685.3. [P81299-3]
NP_740991.1. NM_170993.4. [P81299-4]
UniGeneiCel.17299.

Genome annotation databases

EnsemblMetazoaiC17G10.4b.1; C17G10.4b.1; WBGene00000383. [P81299-1]
C17G10.4b.2; C17G10.4b.2; WBGene00000383. [P81299-1]
GeneIDi173945.
KEGGicel:CELE_C17G10.4.
UCSCiC17G10.4b. c. elegans. [P81299-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY661743 mRNA. Translation: AAT74542.1 .
AY661744 mRNA. Translation: AAT74543.1 .
AY661745 mRNA. Translation: AAT74544.1 .
AY661746 mRNA. Translation: AAT74545.1 .
AY661747 mRNA. Translation: AAT74546.1 .
AF000363 mRNA. Translation: AAB94407.1 .
FO080593 Genomic DNA. Translation: CCD64951.1 .
FO080593 Genomic DNA. Translation: CCD64952.1 .
FO080593 Genomic DNA. Translation: CCD64953.1 .
FO080593 Genomic DNA. Translation: CCD64954.1 .
FO080593 Genomic DNA. Translation: CCD64955.1 .
PIRi E88158.
T34097.
RefSeqi NP_001021969.1. NM_001026798.5. [P81299-5 ]
NP_495084.3. NM_062683.7. [P81299-1 ]
NP_495085.2. NM_062684.5. [P81299-2 ]
NP_495086.1. NM_062685.3. [P81299-3 ]
NP_740991.1. NM_170993.4. [P81299-4 ]
UniGenei Cel.17299.

3D structure databases

ProteinModelPortali P81299.
SMRi P81299. Positions 41-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 39287. 4 interactions.
DIPi DIP-25903N.
MINTi MINT-1063538.

Proteomic databases

PaxDbi P81299.
PRIDEi P81299.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C17G10.4b.1 ; C17G10.4b.1 ; WBGene00000383 . [P81299-1 ]
C17G10.4b.2 ; C17G10.4b.2 ; WBGene00000383 . [P81299-1 ]
GeneIDi 173945.
KEGGi cel:CELE_C17G10.4.
UCSCi C17G10.4b. c. elegans. [P81299-1 ]

Organism-specific databases

CTDi 173945.
WormBasei C17G10.4a ; CE30868 ; WBGene00000383 ; cdc-14.
C17G10.4b ; CE08287 ; WBGene00000383 ; cdc-14.
C17G10.4c ; CE08288 ; WBGene00000383 ; cdc-14.
C17G10.4d ; CE06845 ; WBGene00000383 ; cdc-14.
C17G10.4e ; CE36916 ; WBGene00000383 ; cdc-14.

Phylogenomic databases

eggNOGi COG2453.
GeneTreei ENSGT00390000010254.
InParanoidi P81299.
KOi K06639.
OMAi HYSEAST.

Miscellaneous databases

NextBioi 881775.
PROi P81299.

Family and domain databases

Gene3Di 3.90.190.10. 2 hits.
InterProi IPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR006612. Znf_C2CH.
[Graphical view ]
PANTHERi PTHR23339:SF27. PTHR23339:SF27. 1 hit.
Pfami PF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
PF05485. THAP. 1 hit.
[Graphical view ]
SMARTi SM00692. DM3. 1 hit.
SM00980. THAP. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 2 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50950. ZF_THAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans."
    Saito R.M., Perreault A., Peach B., Satterlee J.S., van den Heuvel S.
    Nat. Cell Biol. 6:777-783(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E), FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  2. Ernsting B.R., Li L., Wishart M.J., Dixon J.E.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo."
    Gruneberg U., Glotzer M., Gartner A., Nigg E.A.
    J. Cell Biol. 158:901-914(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION (ISOFORM C), MUTAGENESIS OF CYS-295.

Entry informationi

Entry nameiCDC14_CAEEL
AccessioniPrimary (citable) accession number: P81299
Secondary accession number(s): P81300
, Q09955, Q09976, Q6DLY2, Q6DLY3, Q6DLY4, Q6DLY5, Q6DLY6, Q8MQD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: October 1, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3