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P81299 (CDC14_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tyrosine-protein phosphatase cdc-14

EC=3.1.3.48
Alternative name(s):
Cell division cycle-related protein 14
Gene names
Name:cdc-14
ORF Names:C17G10.4
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1063 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a role in cell division: functions upstream of the cki-1 Cip/Kip inhibitor to promote accumulation of cki-1 in the nucleus (a hypophosphorylated and stable form of cki-1 is required for developmentally programmed cell-cycle arrest). Isoform c is a regulator of central spindle formation and cytokinesis. Co-localizes with zen-4. Ref.1 Ref.4

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. UniProtKB Q00684

Subcellular location

Isoform c: Cytoplasmcytoskeletonspindle. Note: Localizes to the central spindle in anaphase and to the midbody in telophase. Localizes dynamically and accumulates in the cytoplasm during G1 phase. Ref.1 Ref.4

Disruption phenotype

Worms produce embryos that fail to establish a central spindle and exhibit several mis-localized proteins. This results in extra divisions in multiple lineages, with no apparent defects in mitosis or cell-fate determination. Ref.1

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.

Contains 1 THAP-type zinc finger.

Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from direct assay Ref.4. Source: WormBase

microtubule cytoskeleton organization

Inferred from mutant phenotype Ref.4. Source: UniProtKB

mitotic cell cycle arrest

Inferred from mutant phenotype Ref.1. Source: WormBase

mitotic cytokinesis

Inferred from mutant phenotype Ref.4. Source: WormBase

negative regulation of cell cycle

Inferred from direct assay Ref.1. Source: UniProtKB

peptidyl-tyrosine dephosphorylation

Traceable author statement Ref.4Ref.1. Source: GOC

polar body extrusion after meiotic divisions

Inferred from mutant phenotype Ref.4. Source: WormBase

regulation of cell development

Inferred from mutant phenotype Ref.1. Source: UniProtKB

spindle midzone assembly involved in mitosis

Inferred from mutant phenotype Ref.4. Source: WormBase

   Cellular_componentastral microtubule

Inferred from direct assay Ref.1. Source: WormBase

centrosome

Inferred from direct assay Ref.1. Source: WormBase

cytoplasm

Inferred from direct assay Ref.1. Source: UniProtKB

midbody

Inferred from direct assay Ref.4Ref.1. Source: WormBase

mitotic spindle midzone

Inferred from direct assay Ref.4. Source: WormBase

nucleolus

Inferred from direct assay Ref.1. Source: WormBase

nucleus

Inferred from direct assay Ref.1. Source: WormBase

spindle

Inferred from direct assay Ref.4. Source: UniProtKB

spindle midzone

Inferred from direct assay Ref.1. Source: WormBase

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

phosphatase activity

Inferred from direct assay Ref.4. Source: WormBase

protein tyrosine phosphatase activity

Traceable author statement Ref.4Ref.1. Source: UniProtKB

protein tyrosine/serine/threonine phosphatase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform b (identifier: P81299-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform a (identifier: P81299-2)

The sequence of this isoform differs from the canonical sequence as follows:
     606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS
     694-1063: Missing.
Isoform c (identifier: P81299-3)

The sequence of this isoform differs from the canonical sequence as follows:
     677-681: KMSEN → SCERK
     682-1063: Missing.
Isoform d (identifier: P81299-4)

The sequence of this isoform differs from the canonical sequence as follows:
     606-695: FGLVRVPPDS...VEITTSYVED → ITKCSLTAES...GVCKLVNTLS
     696-1063: Missing.
Isoform e (identifier: P81299-5)

The sequence of this isoform differs from the canonical sequence as follows:
     541-541: T → TGNESYRFHNSLFYE
     606-693: FGLVRVPPDS...VEVEITTSYV → ITKCSLTAES...LLGKRRKTAS
     694-1063: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10631063Probable tyrosine-protein phosphatase cdc-14
PRO_0000094880

Regions

Domain278 – 34063Tyrosine-protein phosphatase
Zinc finger950 – 102475THAP-type

Sites

Active site2951Phosphocysteine intermediate By similarity UniProtKB Q00684

Natural variations

Alternative sequence5411T → TGNESYRFHNSLFYE in isoform e.
VSP_021641
Alternative sequence606 – 69590FGLVR…SYVED → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GVCKLVNTLS in isoform d.
VSP_050492
Alternative sequence606 – 69388FGLVR…TTSYV → ITKCSLTAESKPPKRILSMP GTSKSTSSLKKIQVSRPRPY PSTGVRVELCANGKSYDIRP RKEAHVIPGAGLAANTEALL GKRRKTAS in isoform a and isoform e.
VSP_050491
Alternative sequence677 – 6815KMSEN → SCERK in isoform c.
VSP_050493
Alternative sequence682 – 1063382Missing in isoform c.
VSP_050494
Alternative sequence694 – 1063370Missing in isoform a and isoform e.
VSP_050495
Alternative sequence696 – 1063368Missing in isoform d.
VSP_050496

Experimental info

Mutagenesis2951C → S: Abolishes phosphatase activity (in isoform c). Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: 2B0494459F710197

FASTA1,063120,249
        10         20         30         40         50         60 
MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN INNKFHYEPF 

        70         80         90        100        110        120 
YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD GTGQYDKIRV NTAYVLGAYL 

       130        140        150        160        170        180 
IIYQGFSADD AYLKVSSGET VKFVGFRDAS MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD 

       190        200        210        220        230        240 
FDYEEYEFYE RVENGDFNWI IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST 

       250        260        270        280        290        300 
IVRLNAKNYD ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG 

       310        320        330        340        350        360 
RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS LSQSNGVHLT 

       370        380        390        400        410        420 
QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR RRVQVQNGRS TAPVTIAPAG 

       430        440        450        460        470        480 
TSESRRSTKP SRVVDETALD DQGRSQGDRL LQLKAKHQHE SETTSPNSSS SRRFVKSSTP 

       490        500        510        520        530        540 
QMTVPSQAYL NRNREPIIVT PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR 

       550        560        570        580        590        600 
TPASAVFPSM ASRRSETTRY LSPTTPIKPM SPSYTDGTSP RYKARLRSEN PIGSTTSTPF 

       610        620        630        640        650        660 
SLQPQFGLVR VPPDSPHSIM AHRPPPTTSS RAPLSPHNYS TTQGYSTSSR GLYGDKKPLA 

       670        680        690        700        710        720 
RGSVSTSTLP SMYMTRKMSE NGAVEVEITT SYVEDRKAAE QIDPSSTPPE PKRRFVLVSR 

       730        740        750        760        770        780 
KIVSKEVPSA GPTCSNDPRL NYASTRKLPI KISHRLCQIC RTIKPIADIS MMTDENEKLF 

       790        800        810        820        830        840 
ILLAAIFQRK LFVEECTRIY KQPAVYTCSS HYSEASTAIL NMFGVGGALR IFRARPRKVS 

       850        860        870        880        890        900 
QVEKFASHLM GKQFEEDKLI HSAYMFVLNH PEAGAVTGPI IKEEDIPFEE TVECSSTTAV 

       910        920        930        940        950        960 
NGILPKCFRQ PRKQKLEEDS CSSSILRIIK KTTPSLPNRS YTMINQNMEH PLQCCYCLEV 

       970        980        990       1000       1010       1020 
YEKRYMTQVP KTEQRIARWV AILGEQFRIR LRMKPANYMC RKHFPQADFS SRGRLLKTAV 

      1030       1040       1050       1060 
PNVVSQEKVL AFKLEGNTFV EVKEDNTNIK MEKGTDSEID IEN 

« Hide

Isoform a [UniParc].

Checksum: 9013D05AD9FB560D
Show »

FASTA69377,865
Isoform c [UniParc].

Checksum: 2C056CD6FA03ADDC
Show »

FASTA68176,698
Isoform d [UniParc].

Checksum: E834240A433EDED1
Show »

FASTA69577,995
Isoform e [UniParc].

Checksum: E7C1549ADC2625C3
Show »

FASTA70779,610

References

« Hide 'large scale' references
[1]"The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans."
Saito R.M., Perreault A., Peach B., Satterlee J.S., van den Heuvel S.
Nat. Cell Biol. 6:777-783(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E), FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]Ernsting B.R., Li L., Wishart M.J., Dixon J.E.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[4]"The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo."
Gruneberg U., Glotzer M., Gartner A., Nigg E.A.
J. Cell Biol. 158:901-914(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION (ISOFORM C), MUTAGENESIS OF CYS-295.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY661743 mRNA. Translation: AAT74542.1.
AY661744 mRNA. Translation: AAT74543.1.
AY661745 mRNA. Translation: AAT74544.1.
AY661746 mRNA. Translation: AAT74545.1.
AY661747 mRNA. Translation: AAT74546.1.
AF000363 mRNA. Translation: AAB94407.1.
FO080593 Genomic DNA. Translation: CCD64951.1.
FO080593 Genomic DNA. Translation: CCD64952.1.
FO080593 Genomic DNA. Translation: CCD64953.1.
FO080593 Genomic DNA. Translation: CCD64954.1.
FO080593 Genomic DNA. Translation: CCD64955.1.
PIRE88158.
T34097.
RefSeqNP_001021969.1. NM_001026798.5. [P81299-5]
NP_495084.3. NM_062683.7. [P81299-1]
NP_495085.2. NM_062684.5. [P81299-2]
NP_495086.1. NM_062685.3. [P81299-3]
NP_740991.1. NM_170993.4. [P81299-4]
UniGeneCel.17299.

3D structure databases

ProteinModelPortalP81299.
SMRP81299. Positions 41-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid39287. 4 interactions.
DIPDIP-25903N.
MINTMINT-1063538.

Proteomic databases

PaxDbP81299.
PRIDEP81299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC17G10.4b.1; C17G10.4b.1; WBGene00000383. [P81299-1]
C17G10.4b.2; C17G10.4b.2; WBGene00000383. [P81299-1]
GeneID173945.
KEGGcel:CELE_C17G10.4.
UCSCC17G10.4b. c. elegans. [P81299-1]

Organism-specific databases

CTD173945.
WormBaseC17G10.4a; CE30868; WBGene00000383; cdc-14.
C17G10.4b; CE08287; WBGene00000383; cdc-14.
C17G10.4c; CE08288; WBGene00000383; cdc-14.
C17G10.4d; CE06845; WBGene00000383; cdc-14.
C17G10.4e; CE36916; WBGene00000383; cdc-14.

Phylogenomic databases

eggNOGCOG2453.
GeneTreeENSGT00390000010254.
InParanoidP81299.
KOK06639.
OMAHYSEAST.

Family and domain databases

Gene3D3.90.190.10. 2 hits.
InterProIPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR006612. Znf_C2CH.
[Graphical view]
PANTHERPTHR23339:SF27. PTHR23339:SF27. 1 hit.
PfamPF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
PF05485. THAP. 1 hit.
[Graphical view]
SMARTSM00692. DM3. 1 hit.
SM00980. THAP. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 2 hits.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50950. ZF_THAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio881775.
PROP81299.

Entry information

Entry nameCDC14_CAEEL
AccessionPrimary (citable) accession number: P81299
Secondary accession number(s): P81300 expand/collapse secondary AC list , Q09955, Q09976, Q6DLY2, Q6DLY3, Q6DLY4, Q6DLY5, Q6DLY6, Q8MQD6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: June 11, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase