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Protein

Pectate lyase 1

Gene
N/A
Organism
Juniperus ashei (Ozark white cedar)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has low pectate lyase activity.1 Publication

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Pathwayi: pectin degradation

This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Pectate lyase 1
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi170 – 1701CalciumBy similarity
Metal bindingi194 – 1941CalciumBy similarity
Metal bindingi198 – 1981CalciumBy similarity
Active sitei250 – 2501Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 1 (EC:4.2.2.2)
Alternative name(s):
Major pollen allergen Jun a 1
Allergen: Jun a 1
OrganismiJuniperus ashei (Ozark white cedar)
Taxonomic identifieri13101 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeJuniperus

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE. Causes seasonal allergic rhinitis in North America.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi224 – 2241H → A: Reduced pectate lyase activity. 1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3337. Jun a 1.0101.
3338. Jun a 1.0102.
427. Jun a 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 367346Pectate lyase 1PRO_0000024908Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 451 Publication
Disulfide bondi128 ↔ 1471 Publication
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence analysis
Disulfide bondi306 ↔ 3121 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

Secondary structure

1
367
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 305Combined sources
Turni35 – 373Combined sources
Helixi39 – 446Combined sources
Helixi48 – 503Combined sources
Turni55 – 584Combined sources
Beta strandi59 – 646Combined sources
Helixi79 – 846Combined sources
Beta strandi89 – 957Combined sources
Beta strandi97 – 993Combined sources
Beta strandi109 – 1146Combined sources
Beta strandi120 – 1234Combined sources
Beta strandi129 – 1335Combined sources
Beta strandi135 – 1417Combined sources
Beta strandi143 – 1453Combined sources
Beta strandi152 – 1587Combined sources
Turni159 – 1613Combined sources
Beta strandi162 – 1665Combined sources
Beta strandi173 – 1786Combined sources
Beta strandi180 – 1867Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi193 – 20210Combined sources
Beta strandi204 – 2107Combined sources
Beta strandi212 – 22312Combined sources
Helixi229 – 2335Combined sources
Beta strandi235 – 2406Combined sources
Beta strandi245 – 2495Combined sources
Beta strandi253 – 26311Combined sources
Beta strandi273 – 2786Combined sources
Beta strandi281 – 2866Combined sources
Beta strandi288 – 2903Combined sources
Helixi295 – 2973Combined sources
Beta strandi299 – 3035Combined sources
Helixi309 – 3124Combined sources
Beta strandi317 – 3215Combined sources
Beta strandi323 – 3253Combined sources
Turni344 – 3463Combined sources
Helixi353 – 3553Combined sources
Helixi356 – 3594Combined sources
Turni360 – 3623Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PXZX-ray1.70A/B22-367[»]
ProteinModelPortaliP81294.
SMRiP81294. Positions 22-367.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81294.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni38 – 305268Beta-helixAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPCLIAVL VFLCAIVSCY SDNPIDSCWR GDSNWDQNRM KLADCAVGFG
60 70 80 90 100
SSTMGGKGGD FYTVTSTDDN PVNPTPGTLR YGATREKALW IIFSQNMNIK
110 120 130 140 150
LKMPLYVAGH KTIDGRGADV HLGNGGPCLF MRKVSHVILH SLHIHGCNTS
160 170 180 190 200
VLGDVLVSES IGVEPVHAQD GDAITMRNVT NAWIDHNSLS DCSDGLIDVT
210 220 230 240 250
LGSTGITISN NHFFNHHKVM LLGHDDTYDD DKSMKVTVAF NQFGPNAGQR
260 270 280 290 300
MPRARYGLVH VANNNYDPWN IYAIGGSSNP TILSEGNSFT APSESYKKEV
310 320 330 340 350
TKRIGCESPS ACANWVWRST RDAFINGAYF VSSGKTEETN IYNSNEAFKV
360
ENGNAAPQLT KNAGVVT
Length:367
Mass (Da):39,825
Last modified:December 1, 2000 - v1
Checksum:iFC9B81E675662E49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106663 mRNA. Translation: AAD03609.1.
AF106662 mRNA. Translation: AAD03608.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106663 mRNA. Translation: AAD03609.1.
AF106662 mRNA. Translation: AAD03608.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PXZX-ray1.70A/B22-367[»]
ProteinModelPortaliP81294.
SMRiP81294. Positions 22-367.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3337. Jun a 1.0101.
3338. Jun a 1.0102.
427. Jun a 1.
CAZyiPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Miscellaneous databases

EvolutionaryTraceiP81294.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLY1_JUNAS
AccessioniPrimary (citable) accession number: P81294
Secondary accession number(s): Q9ZNU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: October 14, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.