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Reviewed, UniProtKB/Swiss-Prot P81294 (MPAJ1_JUNAS)

Last modified April 14, 2009. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Major pollen allergen Jun a 1
Alternative name(s):
    Allergen=Jun a 1
OrganismJuniperus ashei (Ozark white cedar)
Taxonomic identifier13101 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Cupressaceae › Juniperus

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Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Allergenic properties

Causes an allergic reaction in human. Causes several seasonal allergies rhinitis in North America. Ref.1 Ref.2

Sequence similarities

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

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Ontologies

Keywords
   DiseaseAllergen
   DomainSignal
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.2
Chain22 – 367346Major pollen allergen Jun a 1
PRO_0000024908

Regions

Region38 – 305268Beta-helix

Amino acid modifications

Glycosylation1481N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Disulfide bond28 ↔ 45 Ref.3
Disulfide bond128 ↔ 147 Ref.3
Disulfide bond306 ↔ 312 Ref.3

Secondary structure

1 .......................................................................... 367
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P81294-1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: FC9B81E675662E49

FASTA36739,825
        10         20         30         40         50         60 
MASPCLIAVL VFLCAIVSCY SDNPIDSCWR GDSNWDQNRM KLADCAVGFG SSTMGGKGGD 

        70         80         90        100        110        120 
FYTVTSTDDN PVNPTPGTLR YGATREKALW IIFSQNMNIK LKMPLYVAGH KTIDGRGADV 

       130        140        150        160        170        180 
HLGNGGPCLF MRKVSHVILH SLHIHGCNTS VLGDVLVSES IGVEPVHAQD GDAITMRNVT 

       190        200        210        220        230        240 
NAWIDHNSLS DCSDGLIDVT LGSTGITISN NHFFNHHKVM LLGHDDTYDD DKSMKVTVAF 

       250        260        270        280        290        300 
NQFGPNAGQR MPRARYGLVH VANNNYDPWN IYAIGGSSNP TILSEGNSFT APSESYKKEV 

       310        320        330        340        350        360 
TKRIGCESPS ACANWVWRST RDAFINGAYF VSSGKTEETN IYNSNEAFKV ENGNAAPQLT 


KNAGVVT

« Hide

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References

[1]"Molecular cloning of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1."
Midoro-Horiuti T.M., Goldblum R.M., Kurosky A., Wood T.G., Brooks E.G.
J. Allergy Clin. Immunol. 104:613-617(1999) [PubMed: 10482836] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-38; 42-50; 58-80; 88-94; 117-124; 134-140; 160-164; 256-263 AND 322-325, ALLERGENICITY.
Tissue: Pollen.
[2]"Isolation and characterization of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1."
Midoro-Horiuti T., Goldblum R.M., Kurosky A., Goetz D.W., Brooks E.G.
J. Allergy Clin. Immunol. 104:608-612(1999) [PubMed: 10482835] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-50, ALLERGENICITY.
Tissue: Pollen.
[3]"Crystal structure of Jun a 1, the major cedar pollen allergen from Juniperus ashei, reveals a parallel beta-helical core."
Czerwinski E.W., Midoro-Horiuti T., White M.A., Brooks E.G., Goldblum R.M.
J. Biol. Chem. 280:3740-3746(2005) [PubMed: 15539389] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 22-367, DISULFIDE BONDS.

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Cross-references

Sequence databases

AF106663 mRNA. Translation: AAD03609.1.
AF106662 mRNA. Translation: AAD03608.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1PXZX-ray1.70A/B22-367[»]
ModBaseSearch...

Protein family/group databases

CAZyPL1. Polysaccharide Lyase Family 1.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMPAJ1_JUNAS
AccessionPrimary (citable) accession number: P81294
Secondary accession number(s): Q9ZNU7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 14, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Allergens

Nomenclature of allergens and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents