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P81288

- RS4_GEOSE

UniProt

P81288 - RS4_GEOSE

Protein

30S ribosomal protein S4

Gene

rpsD

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.By similarity
    With S5 and S12 plays an important role in translational accuracy.By similarity

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-HAMAP
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. response to antibiotic Source: UniProtKB-KW
    2. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    30S ribosomal protein S4
    Alternative name(s):
    BS5
    Gene namesi
    Name:rpsD
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Subcellular locationi

    GO - Cellular componenti

    1. small ribosomal subunit Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 20019930S ribosomal protein S4PRO_0000132338Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity By similarity.By similarity

    Structurei

    Secondary structure

    1
    200
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi46 – 5914
    Turni60 – 623
    Helixi65 – 7511
    Beta strandi78 – 803
    Helixi82 – 9211
    Helixi94 – 1007
    Beta strandi103 – 1064
    Helixi107 – 1159
    Beta strandi119 – 1213
    Beta strandi138 – 1414
    Helixi143 – 1453
    Helixi149 – 1568
    Beta strandi163 – 1686
    Turni169 – 1724
    Beta strandi173 – 1764
    Turni182 – 1843
    Helixi192 – 1998

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1C05NMR-A43-200[»]
    1C06NMR-A43-200[»]
    ProteinModelPortaliP81288.
    SMRiP81288. Positions 43-200.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP81288.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini92 – 15261S4 RNA-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ribosomal protein S4P family.Curated
    Contains 1 S4 RNA-binding domain.Curated

    Family and domain databases

    Gene3Di1.10.1050.10. 1 hit.
    3.10.290.10. 1 hit.
    HAMAPiMF_01306_B. Ribosomal_S4_B.
    InterProiIPR022801. Ribosomal_S4/S9.
    IPR001912. Ribosomal_S4/S9_N.
    IPR005709. Ribosomal_S4_bac-type.
    IPR018079. Ribosomal_S4_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view]
    PANTHERiPTHR11831. PTHR11831. 1 hit.
    PfamiPF00163. Ribosomal_S4. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view]
    SMARTiSM00363. S4. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01017. rpsD_bact. 1 hit.
    PROSITEiPS00632. RIBOSOMAL_S4. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P81288-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARYTGPMWK ISRRLGISLS GTGKELQKRP YPPGQHGPGQ RRKLSEYGLQ    50
    LQEKQKLRHM YGVNERQFRK TFEEAGKMPG KHGENFMILL ESRLDNLVYR 100
    LGLARTRRQA RQLVTHGHII VDGSRVNIPS YRVKPGQTIA VREKSRNLQV 150
    IKEALEANNY IPDYLSFDPE KMEGTYTRLP ERSELPAEIN EALIVEFYSR 200
    Length:200
    Mass (Da):23,196
    Last modified:January 23, 2007 - v3
    Checksum:i8DBED1D1EE76A3F0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti41 – 411Missing AA sequence (PubMed:1764513)Curated

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1C05 NMR - A 43-200 [» ]
    1C06 NMR - A 43-200 [» ]
    ProteinModelPortali P81288.
    SMRi P81288. Positions 43-200.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P81288.

    Family and domain databases

    Gene3Di 1.10.1050.10. 1 hit.
    3.10.290.10. 1 hit.
    HAMAPi MF_01306_B. Ribosomal_S4_B.
    InterProi IPR022801. Ribosomal_S4/S9.
    IPR001912. Ribosomal_S4/S9_N.
    IPR005709. Ribosomal_S4_bac-type.
    IPR018079. Ribosomal_S4_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view ]
    PANTHERi PTHR11831. PTHR11831. 1 hit.
    Pfami PF00163. Ribosomal_S4. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view ]
    SMARTi SM00363. S4. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01017. rpsD_bact. 1 hit.
    PROSITEi PS00632. RIBOSOMAL_S4. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The crystal structure of ribosomal protein S4 reveals a two-domain molecule with an extensive RNA-binding surface: one domain shows structural homology to the ETS DNA-binding motif."
      Davies C., Gerstner R.B., Draper D.E., Ramakrishnan V., White S.W.
      EMBO J. 17:4545-4558(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 42-200.
    2. "Primary structures of ribosomal proteins from the archaebacterium Halobacterium marismortui and the eubacterium Bacillus stearothermophilus."
      Arndt E., Scholzen T., Kromer W., Hatakeyama T., Kimura M.
      Biochimie 73:657-668(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-200.
    3. "Procaryotic ribosomal proteins: N-terminal sequence homologies and structural correspondence of 30 S ribosomal proteins from Escherichia coli and Bacillus stearothermophilus."
      Yaguchi M., Matheson A.T., Visentin L.P.
      FEBS Lett. 46:296-300(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-16.
      Strain: DSM 13240 / CIP 106956 / 10.
    4. "Isolation and characterization of Bacillus stearothermophilus 30S and 50S ribosomal protein mutations."
      Schnier J., Gewitz H.S., Behrens S.E., Lee A., Ginther C., Leighton T.
      J. Bacteriol. 172:7306-7309(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOLATION OF STREPTOMYCIN INDEPENDENT STRAINS.
      Strain: 799.
    5. "Recognition of 16S rRNA by ribosomal protein S4 from Bacillus stearothermophilus."
      Gerstner R.B., Pak Y., Draper D.E.
      Biochemistry 40:7165-7173(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: BINDING TO RRNA.
    6. "Refining the overall structure and subdomain orientation of ribosomal protein S4 delta41 with dipolar couplings measured by NMR in uniaxial liquid crystalline phases."
      Markus M.A., Gerstner R.B., Draper D.E., Torchia D.A.
      J. Mol. Biol. 292:375-387(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 42-199.
    7. "Structural preordering in the N-terminal region of ribosomal protein S4 revealed by heteronuclear NMR spectroscopy."
      Sayers E.W., Gerstner R.B., Draper D.E., Torchia D.A.
      Biochemistry 39:13602-13613(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiRS4_GEOSE
    AccessioniPrimary (citable) accession number: P81288
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 87 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3