30S ribosomal protein S4 - Geobacillus stearothermophilus

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P81288 (RS4_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
30S ribosomal protein S4

Alternative name(s):
BS5

Gene names

Name:

rpsD

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit By similarity. HAMAP-Rule MF_01306_B

With S5 and S12 plays an important role in translational accuracy By similarity. HAMAP-Rule MF_01306_B

Subunit structure

Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity By similarity.

Sequence similarities

Belongs to the ribosomal protein S4P family.

Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.2 Ref.3

Chain

2 – 200

199

30S ribosomal protein S4 HAMAP-Rule MF_01306_B

PRO_0000132338

Regions

Domain

92 – 152

S4 RNA-binding

Experimental info

Sequence conflict

Missing AA sequence Ref.2

Secondary structure

200

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P81288 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8DBED1D1EE76A3F0
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FASTA

200

23,196

        10         20         30         40         50         60 
MARYTGPMWK ISRRLGISLS GTGKELQKRP YPPGQHGPGQ RRKLSEYGLQ LQEKQKLRHM 

        70         80         90        100        110        120 
YGVNERQFRK TFEEAGKMPG KHGENFMILL ESRLDNLVYR LGLARTRRQA RQLVTHGHII 

       130        140        150        160        170        180 
VDGSRVNIPS YRVKPGQTIA VREKSRNLQV IKEALEANNY IPDYLSFDPE KMEGTYTRLP 

       190        200 
ERSELPAEIN EALIVEFYSR

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References

[1]	"The crystal structure of ribosomal protein S4 reveals a two-domain molecule with an extensive RNA-binding surface: one domain shows structural homology to the ETS DNA-binding motif." Davies C., Gerstner R.B., Draper D.E., Ramakrishnan V., White S.W. EMBO J. 17:4545-4558(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 42-200.
[2]	"Primary structures of ribosomal proteins from the archaebacterium Halobacterium marismortui and the eubacterium Bacillus stearothermophilus." Arndt E., Scholzen T., Kromer W., Hatakeyama T., Kimura M. Biochimie 73:657-668(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-200.
[3]	"Procaryotic ribosomal proteins: N-terminal sequence homologies and structural correspondence of 30 S ribosomal proteins from Escherichia coli and Bacillus stearothermophilus." Yaguchi M., Matheson A.T., Visentin L.P. FEBS Lett. 46:296-300(1974) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16. Strain: DSM 13240 / CIP 106956 / 10.
[4]	"Isolation and characterization of Bacillus stearothermophilus 30S and 50S ribosomal protein mutations." Schnier J., Gewitz H.S., Behrens S.E., Lee A., Ginther C., Leighton T. J. Bacteriol. 172:7306-7309(1990) [PubMed] [Europe PMC] [Abstract] Cited for: ISOLATION OF STREPTOMYCIN INDEPENDENT STRAINS. Strain: 799.
[5]	"Recognition of 16S rRNA by ribosomal protein S4 from Bacillus stearothermophilus." Gerstner R.B., Pak Y., Draper D.E. Biochemistry 40:7165-7173(2001) [PubMed] [Europe PMC] [Abstract] Cited for: BINDING TO RRNA.
[6]	"Refining the overall structure and subdomain orientation of ribosomal protein S4 delta41 with dipolar couplings measured by NMR in uniaxial liquid crystalline phases." Markus M.A., Gerstner R.B., Draper D.E., Torchia D.A. J. Mol. Biol. 292:375-387(1999) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 42-199.
[7]	"Structural preordering in the N-terminal region of ribosomal protein S4 revealed by heteronuclear NMR spectroscopy." Sayers E.W., Gerstner R.B., Draper D.E., Torchia D.A. Biochemistry 39:13602-13613(2000) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C05	NMR	-	A	43-200	[»]
1C06	NMR	-	A	43-200	[»]

ProteinModelPortal

P81288.

SMR

P81288. Positions 43-200.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.1050.10. 1 hit.
3.10.290.10. 1 hit.

HAMAP

MF_01306_B. Ribosomal_S4_B.

InterPro

IPR022801. Ribosomal_S4/S9.
IPR001912. Ribosomal_S4/S9_N.
IPR005709. Ribosomal_S4_bac-type.
IPR018079. Ribosomal_S4_CS.
IPR002942. S4_RNA-bd.
[Graphical view]

PANTHER

PTHR11831. PTHR11831. 1 hit.

Pfam

PF00163. Ribosomal_S4. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]

SMART

SM00363. S4. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01017. rpsD_bact. 1 hit.

PROSITE

PS00632. RIBOSOMAL_S4. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P81288.

Entry information

Entry name

RS4_GEOSE

Accession

Primary (citable) accession number: P81288

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

3D structure databases

Protocols and materials databases

Family and domain databases

Other

Entry information

Relevant documents