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P81288 (RS4_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
30S ribosomal protein S4
Alternative name(s):
BS5
Gene names
Name:rpsD
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit By similarity. HAMAP-Rule MF_01306_B

With S5 and S12 plays an important role in translational accuracy By similarity. HAMAP-Rule MF_01306_B

Subunit structure

Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity By similarity.

Sequence similarities

Belongs to the ribosomal protein S4P family.

Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
   Biological processAntibiotic resistance
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processresponse to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

translation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentsmall ribosomal subunit

Inferred from electronic annotation. Source: InterPro

   Molecular_functionrRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2 Ref.3
Chain2 – 20019930S ribosomal protein S4 HAMAP-Rule MF_01306_B
PRO_0000132338

Regions

Domain92 – 15261S4 RNA-binding

Experimental info

Sequence conflict411Missing AA sequence Ref.2

Secondary structure

............................... 200
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P81288 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8DBED1D1EE76A3F0

FASTA20023,196
        10         20         30         40         50         60 
MARYTGPMWK ISRRLGISLS GTGKELQKRP YPPGQHGPGQ RRKLSEYGLQ LQEKQKLRHM 

        70         80         90        100        110        120 
YGVNERQFRK TFEEAGKMPG KHGENFMILL ESRLDNLVYR LGLARTRRQA RQLVTHGHII 

       130        140        150        160        170        180 
VDGSRVNIPS YRVKPGQTIA VREKSRNLQV IKEALEANNY IPDYLSFDPE KMEGTYTRLP 

       190        200 
ERSELPAEIN EALIVEFYSR 

« Hide

References

[1]"The crystal structure of ribosomal protein S4 reveals a two-domain molecule with an extensive RNA-binding surface: one domain shows structural homology to the ETS DNA-binding motif."
Davies C., Gerstner R.B., Draper D.E., Ramakrishnan V., White S.W.
EMBO J. 17:4545-4558(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 42-200.
[2]"Primary structures of ribosomal proteins from the archaebacterium Halobacterium marismortui and the eubacterium Bacillus stearothermophilus."
Arndt E., Scholzen T., Kromer W., Hatakeyama T., Kimura M.
Biochimie 73:657-668(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-200.
[3]"Procaryotic ribosomal proteins: N-terminal sequence homologies and structural correspondence of 30 S ribosomal proteins from Escherichia coli and Bacillus stearothermophilus."
Yaguchi M., Matheson A.T., Visentin L.P.
FEBS Lett. 46:296-300(1974) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-16.
Strain: DSM 13240 / CIP 106956 / 10.
[4]"Isolation and characterization of Bacillus stearothermophilus 30S and 50S ribosomal protein mutations."
Schnier J., Gewitz H.S., Behrens S.E., Lee A., Ginther C., Leighton T.
J. Bacteriol. 172:7306-7309(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOLATION OF STREPTOMYCIN INDEPENDENT STRAINS.
Strain: 799.
[5]"Recognition of 16S rRNA by ribosomal protein S4 from Bacillus stearothermophilus."
Gerstner R.B., Pak Y., Draper D.E.
Biochemistry 40:7165-7173(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: BINDING TO RRNA.
[6]"Refining the overall structure and subdomain orientation of ribosomal protein S4 delta41 with dipolar couplings measured by NMR in uniaxial liquid crystalline phases."
Markus M.A., Gerstner R.B., Draper D.E., Torchia D.A.
J. Mol. Biol. 292:375-387(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 42-199.
[7]"Structural preordering in the N-terminal region of ribosomal protein S4 revealed by heteronuclear NMR spectroscopy."
Sayers E.W., Gerstner R.B., Draper D.E., Torchia D.A.
Biochemistry 39:13602-13613(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1C05NMR-A43-200[»]
1C06NMR-A43-200[»]
ProteinModelPortalP81288.
SMRP81288. Positions 43-200.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1050.10. 1 hit.
3.10.290.10. 1 hit.
HAMAPMF_01306_B. Ribosomal_S4_B.
InterProIPR022801. Ribosomal_S4/S9.
IPR001912. Ribosomal_S4/S9_N.
IPR005709. Ribosomal_S4_bac-type.
IPR018079. Ribosomal_S4_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERPTHR11831. PTHR11831. 1 hit.
PfamPF00163. Ribosomal_S4. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsTIGR01017. rpsD_bact. 1 hit.
PROSITEPS00632. RIBOSOMAL_S4. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP81288.

Entry information

Entry nameRS4_GEOSE
AccessionPrimary (citable) accession number: P81288
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references