Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P81286 (PLMN_SHEEP)

Last modified June 16, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Plasminogen
    EC=3.4.21.7
Gene names
Name: PLG
OrganismOvis aries (Sheep)
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length343 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation; in ovulation it weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. It cleaves fibrin, fibronectin, thrombospondin, laminin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4.

Catalytic activity

Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.

Enzyme regulation

Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Cannot be activated with streptokinase.

Subunit structure

Interacts with CSPG4 By similarity.

Subcellular location

Secreted.

Domain

Kringle domains mediate interaction with CSPG4 By similarity.

Miscellaneous

Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot.

Sequence similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Contains at least 2 kringle domains.

Contains 1 peptidase S1 domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›343›343Plasminogen
PRO_0000088704

Regions

Domain‹1 – 17›17Kringle 4
Domain41 – 12080Kringle 5
Domain114 – 341228Peptidase S1
Region‹1 – 140›140Plasmin heavy chain A
Region141 – ›343›203Plasmin light chain B

Sites

Active site1551Charge relay system By similarity
Active site1981Charge relay system By similarity
Active site2931Charge relay system By similarity

Amino acid modifications

Disulfide bond15 ↔ 94 By similarity
Disulfide bond36 ↔ 77 By similarity
Disulfide bond65 ↔ 89 By similarity
Disulfide bond140 ↔ 156 By similarity
Disulfide bond232 ↔ 299 By similarity
Disulfide bond262 ↔ 278 By similarity
Disulfide bond289 ↔ 317 By similarity

Experimental info

Non-terminal residue11
Non-terminal residue3431

Sequences

Sequence LengthMass (Da)Tools
P81286-1 [UniParc].

Last modified December 15, 1998. Version 1.
Checksum: 8DF6EBA92D596EE0

FASTA34337,662
        10         20         30         40         50         60 
APQAPSVENP PEADCMLGIG KGYRGKKATT VAGVPCQEWA AQEPHRHGIF TPETNPRAGL 

        70         80         90        100        110        120 
EKNYCRNPDG DVNGPWCYTT NPRKLFDYCD IPQCESSFDC GKPKVEPKKC PARVVGGCVA 

       130        140        150        160        170        180 
TPHSWPWQVS LRRRSREHFC GGTLISPEWV LTAAHCLDSI LGPSFYTVIL GAHYEMAREA 

       190        200        210        220        230        240 
SVQEIPVSRL FLEPSRADIA LLKLSSPAVI TDEVIPACLP SPNYVVADKT VCYITGWGET 

       250        260        270        280        290        300 
QGTFGVGRLK EARLPVIENK VCNRYEYLNG RVKSTELCAG DLAGGTDSCQ GDSGGPLVCF 

       310        320        330        340 
EKDKYILQGV TSWGLGCARP NKPGVYVRVS TYVPWIEETM RRY 

« Hide

References

[1]"Complete amino acid sequence of ovine miniplasminogen."
Schaller J., Straub C., Kampfer U., Rickli E.E.
Protein Seq. Data Anal. 5:21-25(1992) [PubMed: 1492092] [Abstract]
Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases

PIRB61545.

3D structure databases

HSSPHSSP built from PDB template 5HPG based on UniProtKB P00747.
SMRP81286. Positions 97-341.
ModBaseSearch...

Protein family/group databases

MEROPSS01.233.

Phylogenomic databases

HOVERGENP81286.

Enzyme and pathway databases

BRENDA3.4.21.7. 271.

Family and domain databases

InterProIPR000001. Kringle.
IPR018056. Kringle_CS.
IPR018059. Kringle_sub.
IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
IPR003966. Peptidase_S1A_prothrombin.
[Graphical view]
Gene3DG3DSA:2.40.20.10. Kringle. 1 hit.
PfamPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
PR00018. KRINGLE.
PR01505. PROTHROMBIN.
ProDomPD000395. Kringle. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLMN_SHEEP
AccessionPrimary (citable) accession number: P81286
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents