P81286 (PLMN_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plasminogen
EC=3.4.21.7

Gene names

Name:

PLG

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	343 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells By similarity.
Catalytic activity	Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.
Enzyme regulation	Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Cannot be activated with streptokinase.
Subunit structure	Interacts with CSPG4 and AMOT. Interacts (via the Kringle domains) with HRG; the interaction tethers PLG to the cell surface and enhances its activation By similarity.
Subcellular location	Secreted By similarity. Note: Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface By similarity.
Domain	Kringle domains mediate interaction with CSPG4 By similarity.
Miscellaneous	Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot.
Sequence similarities	Belongs to the peptidase S1 family. Plasminogen subfamily. Contains at least 2 kringle domains. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Blood coagulation Fibrinolysis Tissue remodeling
Cellular component	Secreted
Domain	Kringle Repeat
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW fibrinolysis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW tissue remodeling Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›343	›343	Plasminogen		PRO_0000088704
Regions
	Domain	‹1 – 17	›17	Kringle 4
	Domain	41 – 120	80	Kringle 5
	Domain	114 – 341	228	Peptidase S1
	Region	‹1 – 140	›140	Plasmin heavy chain A
	Region	141 – ›343	›203	Plasmin light chain B
Sites
	Active site	155	1	Charge relay system By similarity
	Active site	198	1	Charge relay system By similarity
	Active site	293	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	15 ↔ 94		By similarity
	Disulfide bond	36 ↔ 77		By similarity
	Disulfide bond	65 ↔ 89		By similarity
	Disulfide bond	140 ↔ 156		By similarity
	Disulfide bond	232 ↔ 299		By similarity
	Disulfide bond	262 ↔ 278		By similarity
	Disulfide bond	289 ↔ 317		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	343	1

Sequences

	Sequence		Length	Mass (Da)
	P81286 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 8DF6EBA92D596EE0	FASTA	343	37,662
	10 20 30 40 50 60 APQAPSVENP PEADCMLGIG KGYRGKKATT VAGVPCQEWA AQEPHRHGIF TPETNPRAGL 70 80 90 100 110 120 EKNYCRNPDG DVNGPWCYTT NPRKLFDYCD IPQCESSFDC GKPKVEPKKC PARVVGGCVA 130 140 150 160 170 180 TPHSWPWQVS LRRRSREHFC GGTLISPEWV LTAAHCLDSI LGPSFYTVIL GAHYEMAREA 190 200 210 220 230 240 SVQEIPVSRL FLEPSRADIA LLKLSSPAVI TDEVIPACLP SPNYVVADKT VCYITGWGET 250 260 270 280 290 300 QGTFGVGRLK EARLPVIENK VCNRYEYLNG RVKSTELCAG DLAGGTDSCQ GDSGGPLVCF 310 320 330 340 EKDKYILQGV TSWGLGCARP NKPGVYVRVS TYVPWIEETM RRY

References

[1]	"Complete amino acid sequence of ovine miniplasminogen." Schaller J., Straub C., Kampfer U., Rickli E.E. Protein Seq. Data Anal. 5:21-25(1992) [PubMed: 1492092] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	B61545.
3D structure databases
ProteinModelPortal	P81286.
SMR	P81286. Positions 14-341.
ModBase	Search...
Protein family/group databases
MEROPS	S01.233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008633.
Family and domain databases
InterPro	IPR000001. Kringle. IPR013806. Kringle-like. IPR018056. Kringle_CS. IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
SMART	SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF57440. Kringle-like. 1 hit. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLMN_SHEEP

Accession

Primary (citable) accession number: P81286

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program