Reviewed, UniProtKB/Swiss-Prot P81237 (PA22_ACAAN)

Names and origin

Protein names	Recommended name:     Phospholipase A2     EC=3.1.1.4 Alternative name(s):     Acanthin II     Phosphatidylcholine 2-acylhydrolase
Organism	Acanthophis antarcticus (Common death adder)
Taxonomic identifier	8605 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Acanthophis

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Exhibits antiplatelet activity.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Phospholipase A2		PRO_0000161592
Sites
	Active site	48	1	By similarity
	Active site	92	1	By similarity
	Metal binding	28	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	30	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	32	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	49	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 71		By similarity
	Disulfide bond	27 ↔ 117		By similarity
	Disulfide bond	29 ↔ 45		By similarity
	Disulfide bond	44 ↔ 98		By similarity
	Disulfide bond	51 ↔ 91		By similarity
	Disulfide bond	60 ↔ 84		By similarity
	Disulfide bond	78 ↔ 89		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81237-1 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: E1477D186C06B3EB	FASTA	118	12,884
	10 20 30 40 50 60 NLYQFGGMIQ CANKGARSWL SYVNYGCYCG WGGSGTPVDE LDRCCQIHDN CYGEAEKKRC 70 80 90 100 110 GPKMTLYSWE CANDVPVCNS KSACEGFVCD CDAAAAKCFA KAPYNKNNIG IGSKTRCQ

References

[1]

"Purification, characterization, and amino acid sequence determination of acanthins, potent inhibitors of platelet aggregation from Acanthophis antarcticus (common death adder) venom." Chow G., Subburaju S., Kini R.M. Arch. Biochem. Biophys. 354:232-238(1998) [PubMed: 9637731] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

3D structure databases
SMR	P81237. Positions 1-118.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P81237.
Enzyme and pathway databases
BRENDA	3.1.1.4. 291618.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA22_ACAAN

Accession

Primary (citable) accession number: P81237

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)