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P81236 (PA2B1_ACAAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Basic phospholipase A2 acanthin-1
Short name=svPLA2
EC=3.1.1.4
Alternative name(s):
Acanthin I
Phosphatidylcholine 2-acylhydrolase
OrganismAcanthophis antarcticus (Common death adder)
Taxonomic identifier8605 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Acanthophis

Protein attributes

Sequence length119 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Snake venom phospholipase A2 (PLA2) that potently inhibits ADP-(IC50=10 nM) and collagen-induced (IC50=7 nM) platelet aggregation. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Ref.1

Catalytic activity

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Cofactor

Binds 1 calcium ion By similarity.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Sequence similarities

Belongs to the phospholipase A2 family. Group I subfamily. D49 sub-subfamily.

Mass spectrometry

Molecular mass is 12844.58 Da from positions 1 - 119. Determined by ESI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 119119Basic phospholipase A2 acanthin-1
PRO_0000161591

Sites

Active site481 By similarity
Active site931 By similarity
Metal binding281Calcium; via carbonyl oxygen By similarity
Metal binding301Calcium; via carbonyl oxygen By similarity
Metal binding321Calcium; via carbonyl oxygen By similarity
Metal binding491Calcium By similarity

Amino acid modifications

Disulfide bond11 ↔ 71 By similarity
Disulfide bond27 ↔ 118 By similarity
Disulfide bond29 ↔ 45 By similarity
Disulfide bond44 ↔ 99 By similarity
Disulfide bond51 ↔ 92 By similarity
Disulfide bond60 ↔ 85 By similarity
Disulfide bond78 ↔ 90 By similarity

Sequences

Sequence LengthMass (Da)Tools
P81236 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 229241793FA826F7

FASTA11912,857
        10         20         30         40         50         60 
DLFQFGGMIG CANKGARSWL SYVNYGCYCG WGGSGTPVDE LDRCCQIHDN CYGEAEKKQC 

        70         80         90        100        110 
GPKMTSYSWK CANDVPVCND SKSACKGFVC DCDAAAAKCF AKAPYNKNNI GIGSKTRCQ

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References

[1]"Purification, characterization, and amino acid sequence determination of acanthins, potent inhibitors of platelet aggregation from Acanthophis antarcticus (common death adder) venom."
Chow G., Subburaju S., Kini R.M.
Arch. Biochem. Biophys. 354:232-238(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY.
Tissue: Venom.

Cross-references

3D structure databases

ProteinModelPortalP81236.
SMRP81236. Positions 1-119.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG008137.

Family and domain databases

Gene3D1.20.90.10. 1 hit.
InterProIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERPTHR11716. PTHR11716. 1 hit.
PfamPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSPR00389. PHPHLIPASEA2.
SMARTSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMSSF48619. PhospholipaseA2. 1 hit.
PROSITEPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePA2B1_ACAAN
AccessionPrimary (citable) accession number: P81236
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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