Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc metalloproteinase aureolysin

Gene

aur

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residues. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 3 Ca2+ ions per subunit.
  • Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi348 – 3481Calcium 1
Metal bindingi352 – 3521Zinc; catalytic
Active sitei353 – 3531
Metal bindingi356 – 3561Zinc; catalytic
Metal bindingi376 – 3761Zinc; catalytic
Metal bindingi387 – 3871Calcium 1
Metal bindingi387 – 3871Calcium 2
Metal bindingi389 – 3891Calcium 2
Metal bindingi390 – 3901Calcium 1
Metal bindingi390 – 3901Calcium 2
Metal bindingi392 – 3921Calcium 1; via carbonyl oxygen
Metal bindingi395 – 3951Calcium 1
Metal bindingi395 – 3951Calcium 2
Metal bindingi398 – 3981Calcium 3; via carbonyl oxygen
Metal bindingi399 – 3991Calcium 3
Metal bindingi402 – 4021Calcium 3; via carbonyl oxygen
Metal bindingi405 – 4051Calcium 3
Active sitei436 – 4361Proton donor

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase aureolysin (EC:3.4.24.29)
Alternative name(s):
Staphylococcus aureus neutral proteinase
Gene namesi
Name:aur
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Propeptidei28 – 2091821 PublicationPRO_0000028614Add
BLAST
Chaini210 – 509300Zinc metalloproteinase aureolysinPRO_0000028615Add
BLAST

Keywords - PTMi

Zymogen

Structurei

Secondary structure

1
509
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi210 – 2167Combined sources
Beta strandi222 – 2309Combined sources
Beta strandi233 – 2386Combined sources
Beta strandi240 – 2434Combined sources
Beta strandi245 – 2506Combined sources
Turni252 – 2543Combined sources
Beta strandi257 – 2593Combined sources
Beta strandi261 – 2677Combined sources
Helixi270 – 2723Combined sources
Helixi273 – 29321Combined sources
Beta strandi305 – 3095Combined sources
Beta strandi323 – 3253Combined sources
Beta strandi327 – 3326Combined sources
Beta strandi337 – 3415Combined sources
Helixi343 – 3453Combined sources
Helixi347 – 36115Combined sources
Helixi369 – 38618Combined sources
Beta strandi391 – 3944Combined sources
Turni395 – 3973Combined sources
Beta strandi407 – 4115Combined sources
Helixi413 – 4164Combined sources
Helixi422 – 4243Combined sources
Helixi430 – 4389Combined sources
Helixi440 – 45314Combined sources
Helixi455 – 46814Combined sources
Helixi476 – 49015Combined sources
Helixi493 – 50412Combined sources
Turni505 – 5073Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BQBX-ray1.72A209-509[»]
ProteinModelPortaliP81177.
SMRiP81177. Positions 209-509.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81177.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKFSRYAFT SMATVTLLSS LTPAALASDT NHKPATSDIN FEITQKSDAV
60 70 80 90 100
KALKELPKSE NVKNHYQDYS VTDVKTDKKG FTHYTLQPSV DGVHAPDKEV
110 120 130 140 150
KVHADKSGKV VLINGDTDAK KVKPTNKVTL SKDEAADKAF NAVKIDKNKA
160 170 180 190 200
KNLQDDVIKE NKVEIDGDSN KYIYNIELIT VTPEISHWKV KIDADTGAVV
210 220 230 240 250
EKTNLVKEAA ATGTGKGVLG DTKDININSI DGGFSLEDLT HQGKLSAYNF
260 270 280 290 300
NDQTGQATLI TNEDENFVKD DQRAGVDANY YAKQTYDYYK NTFGRESYDN
310 320 330 340 350
HGSPIVSLTH VNHYGGQDNR NNAAWIGDKM IYGDGDGRTF TNLSGANDVV
360 370 380 390 400
AHELTHGVTQ ETANLEYKDQ SGALNESFSD VFGYFVDDED FLMGEDVYTP
410 420 430 440 450
GKEGDALRSM SNPEQFGQPS HMKDYVYTEK DNGGVHTNSG IPNKAAYNVI
460 470 480 490 500
QAIGKSKSEQ IYYRALTEYL TSNSNFKDCK DALYQAAKDL YDEQTAEQVY

EAWNEVGVE
Length:509
Mass (Da):56,321
Last modified:December 1, 2000 - v2
Checksum:iC622D5B7228108E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti354 – 3541L → I AA sequence (PubMed:9753696).Curated
Sequence conflicti361 – 3611E → Q AA sequence (PubMed:9753696).Curated
Sequence conflicti479 – 4791C → L AA sequence (PubMed:9753696).Curated
Sequence conflicti492 – 4932DE → EQ AA sequence (PubMed:9753696).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249166 Genomic DNA. Translation: CAB59567.1.
AJ249167 Genomic DNA. Translation: CAB59568.1.
RefSeqiWP_001225040.1. NZ_LN626917.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249166 Genomic DNA. Translation: CAB59567.1.
AJ249167 Genomic DNA. Translation: CAB59568.1.
RefSeqiWP_001225040.1. NZ_LN626917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BQBX-ray1.72A209-509[»]
ProteinModelPortaliP81177.
SMRiP81177. Positions 209-509.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM04.009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP81177.

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAURE_STAAU
AccessioniPrimary (citable) accession number: P81177
Secondary accession number(s): Q9R2Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: March 16, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.