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Protein

Zinc metalloproteinase aureolysin

Gene

aur

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residues. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 3 Ca2+ ions per subunit.
  • Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi348Calcium 11
Metal bindingi352Zinc; catalytic1
Active sitei3531
Metal bindingi356Zinc; catalytic1
Metal bindingi376Zinc; catalytic1
Metal bindingi387Calcium 11
Metal bindingi387Calcium 21
Metal bindingi389Calcium 21
Metal bindingi390Calcium 11
Metal bindingi390Calcium 21
Metal bindingi392Calcium 1; via carbonyl oxygen1
Metal bindingi395Calcium 11
Metal bindingi395Calcium 21
Metal bindingi398Calcium 3; via carbonyl oxygen1
Metal bindingi399Calcium 31
Metal bindingi402Calcium 3; via carbonyl oxygen1
Metal bindingi405Calcium 31
Active sitei436Proton donor1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase aureolysin (EC:3.4.24.29)
Alternative name(s):
Staphylococcus aureus neutral proteinase
Gene namesi
Name:aur
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002861428 – 2091 PublicationAdd BLAST182
ChainiPRO_0000028615210 – 509Zinc metalloproteinase aureolysinAdd BLAST300

Keywords - PTMi

Zymogen

Structurei

Secondary structure

1509
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi210 – 216Combined sources7
Beta strandi222 – 230Combined sources9
Beta strandi233 – 238Combined sources6
Beta strandi240 – 243Combined sources4
Beta strandi245 – 250Combined sources6
Turni252 – 254Combined sources3
Beta strandi257 – 259Combined sources3
Beta strandi261 – 267Combined sources7
Helixi270 – 272Combined sources3
Helixi273 – 293Combined sources21
Beta strandi305 – 309Combined sources5
Beta strandi323 – 325Combined sources3
Beta strandi327 – 332Combined sources6
Beta strandi337 – 341Combined sources5
Helixi343 – 345Combined sources3
Helixi347 – 361Combined sources15
Helixi369 – 386Combined sources18
Beta strandi391 – 394Combined sources4
Turni395 – 397Combined sources3
Beta strandi407 – 411Combined sources5
Helixi413 – 416Combined sources4
Helixi422 – 424Combined sources3
Helixi430 – 438Combined sources9
Helixi440 – 453Combined sources14
Helixi455 – 468Combined sources14
Helixi476 – 490Combined sources15
Helixi493 – 504Combined sources12
Turni505 – 507Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BQBX-ray1.72A209-509[»]
ProteinModelPortaliP81177.
SMRiP81177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81177.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKFSRYAFT SMATVTLLSS LTPAALASDT NHKPATSDIN FEITQKSDAV
60 70 80 90 100
KALKELPKSE NVKNHYQDYS VTDVKTDKKG FTHYTLQPSV DGVHAPDKEV
110 120 130 140 150
KVHADKSGKV VLINGDTDAK KVKPTNKVTL SKDEAADKAF NAVKIDKNKA
160 170 180 190 200
KNLQDDVIKE NKVEIDGDSN KYIYNIELIT VTPEISHWKV KIDADTGAVV
210 220 230 240 250
EKTNLVKEAA ATGTGKGVLG DTKDININSI DGGFSLEDLT HQGKLSAYNF
260 270 280 290 300
NDQTGQATLI TNEDENFVKD DQRAGVDANY YAKQTYDYYK NTFGRESYDN
310 320 330 340 350
HGSPIVSLTH VNHYGGQDNR NNAAWIGDKM IYGDGDGRTF TNLSGANDVV
360 370 380 390 400
AHELTHGVTQ ETANLEYKDQ SGALNESFSD VFGYFVDDED FLMGEDVYTP
410 420 430 440 450
GKEGDALRSM SNPEQFGQPS HMKDYVYTEK DNGGVHTNSG IPNKAAYNVI
460 470 480 490 500
QAIGKSKSEQ IYYRALTEYL TSNSNFKDCK DALYQAAKDL YDEQTAEQVY

EAWNEVGVE
Length:509
Mass (Da):56,321
Last modified:December 1, 2000 - v2
Checksum:iC622D5B7228108E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti354L → I AA sequence (PubMed:9753696).Curated1
Sequence conflicti361E → Q AA sequence (PubMed:9753696).Curated1
Sequence conflicti479C → L AA sequence (PubMed:9753696).Curated1
Sequence conflicti492 – 493DE → EQ AA sequence (PubMed:9753696).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249166 Genomic DNA. Translation: CAB59567.1.
AJ249167 Genomic DNA. Translation: CAB59568.1.
RefSeqiWP_001225040.1. NZ_LWRD01000012.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249166 Genomic DNA. Translation: CAB59567.1.
AJ249167 Genomic DNA. Translation: CAB59568.1.
RefSeqiWP_001225040.1. NZ_LWRD01000012.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BQBX-ray1.72A209-509[»]
ProteinModelPortaliP81177.
SMRiP81177.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM04.009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP81177.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAURE_STAAU
AccessioniPrimary (citable) accession number: P81177
Secondary accession number(s): Q9R2Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.