Reviewed, UniProtKB/Swiss-Prot P81160 (DEJP_DROME)

Names and origin

Protein names

Recommended name:
    Ductus ejaculatorius peptide 99B

Gene names

Name:	Dup99B
ORF Names:	CG33495

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	54 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Induces post-mating responses; increased oviposition and reduced receptivity. Ref.4
Subcellular location	Secreted. Ref.4
Tissue specificity	Ductus ejaculatorius. Ref.4
Sequence similarities	To paragonial peptide B.

Ontologies

Keywords
Biological process	Behavior
Cellular component	Secreted
Domain	Signal
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	regulation of female receptivity, post-mating Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Peptide	22 – 52	31	Ductus ejaculatorius peptide 99B		PRO_0000021103
	Propeptide	53 – 54	2			PRO_0000021104
Amino acid modifications
	Modified residue	22	1	Pyrrolidone carboxylic acid
	Glycosylation	25	1	N-linked (GlcNAc...)
	Disulfide bond	40 ↔ 52		Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P81160-1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 7D764665A2D5EFB5	FASTA	54	6,245
	10 20 30 40 50 MKTPLFLLLV VLASLLGLAL SQDRNDTEWI QSQKDREKWC RLNLGPYLGG RCRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-37. Strain: Berkeley.
[4]	"Ductus ejaculatorius peptide 99B (DUP99B), a novel Drosophila melanogaster sex-peptide pheromone." Saudan P., Hauck K., Soller M., Choffat Y., Ottiger M., Sporri M., Ding Z., Hess D., Gehrig P.M., Klauser S., Hunziker P., Kubli E. Eur. J. Biochem. 269:989-997(2002) [PubMed: 11846801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-45, PROTEIN SEQUENCE OF 30-52, GLYCOSYLATION, DISULFIDE BONDS, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Oregon-R. Tissue: Ductus ejaculatorius.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014297 Genomic DNA. Translation: AAS65225.1. BT032714 mRNA. Translation: ACD81728.1. Different initiation. BT032724 mRNA. Translation: ACD81738.1. Different initiation. BT032738 mRNA. Translation: ACD81752.1. Different initiation. BT032746 mRNA. Translation: ACD81760.1. Different initiation.
RefSeq	NP_996305.1.
UniGene	Dm.21449
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0024381. Drosophila melanogaster. [Contig view]
GeneID	2768691.
KEGG	dme:Dmel_CG33495.
NMPDR	fig|7227.3.peg.15265.
Organism-specific databases
FlyBase	FBgn0250832. Dup99B.
Phylogenomic databases
HOGENOM	P81160.
Gene expression databases
GermOnline	CG33495. Drosophila melanogaster.
Family and domain databases
InterPro	IPR012608. Sex_peptide. [Graphical view]
Pfam	PF08138. Sex_peptide. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	848126.

Entry information

Entry name

DEJP_DROME

Accession

Primary (citable) accession number: P81160
Secondary accession number(s): B3DMS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 10, 2004
Last modified:	June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project