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Protein

Voltage-dependent anion-selective channel protein 2

Gene

Vdac2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei85 – 851Involved in hexokinase bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi254 – 2563NADBy similarity
Nucleotide bindingi272 – 2765NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

NAD, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent anion-selective channel protein 2
Short name:
VDAC-2
Alternative name(s):
B36-VDAC
Outer mitochondrial membrane protein porin 2
Gene namesi
Name:Vdac2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi621576. Vdac2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei38 – 4710Beta strandedBy similarity
Transmembranei51 – 599Beta strandedBy similarity
Transmembranei66 – 7611Beta strandedBy similarityAdd
BLAST
Transmembranei81 – 888Beta strandedBy similarity
Transmembranei92 – 10110Beta strandedBy similarity
Transmembranei107 – 11610Beta strandedBy similarity
Transmembranei123 – 13210Beta strandedBy similarity
Transmembranei135 – 1428Beta strandedBy similarity
Transmembranei149 – 1579Beta strandedBy similarity
Transmembranei162 – 1709Beta strandedBy similarity
Transmembranei175 – 18713Beta strandedBy similarityAdd
BLAST
Transmembranei190 – 1978Beta strandedBy similarity
Transmembranei201 – 21010Beta strandedBy similarity
Transmembranei214 – 22310Beta strandedBy similarity
Transmembranei230 – 23910Beta strandedBy similarity
Transmembranei243 – 2508Beta strandedBy similarity
Transmembranei254 – 26310Beta strandedBy similarity
Transmembranei266 – 27510Beta strandedBy similarity
Transmembranei285 – 29410Beta strandedBy similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Voltage-dependent anion-selective channel protein 2PRO_0000050509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321N6-acetyllysine; alternateBy similarity
Modified residuei32 – 321N6-succinyllysine; alternateBy similarity
Cross-linki32 – 32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki65 – 65Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei121 – 1211N6-acetyllysine; alternateBy similarity
Cross-linki121 – 121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki122 – 122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei237 – 2371Phosphotyrosine1 Publication
Cross-linki278 – 278Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP81155.
PRIDEiP81155.

2D gel databases

World-2DPAGE0004:P81155.

PTM databases

iPTMnetiP81155.
PhosphoSiteiP81155.
SwissPalmiP81155.

Expressioni

Tissue specificityi

Highly expressed in heart, kidney, brain and ascitic tumor with very low levels in liver. Expressed in the head region of epididymal sperm.2 Publications

Gene expression databases

GenevisibleiP81155. RN.

Interactioni

Subunit structurei

Interacts with hexokinases.By similarity

Protein-protein interaction databases

BioGridi249747. 2 interactions.
IntActiP81155. 2 interactions.
MINTiMINT-4617801.
STRINGi10116.ENSRNOP00000018462.

Family & Domainsi

Domaini

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiP81155.
KOiK15040.
OMAiMCVPPSY.
OrthoDBiEOG7P8P8G.
PhylomeDBiP81155.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030277. VDAC2.
[Graphical view]
PANTHERiPTHR11743:SF12. PTHR11743:SF12. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P81155-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAECCVPVCQ RPICIPPPYA DLGKAARDIF NKGFGFGLVK LDVKTKSCSG
60 70 80 90 100
VEFSTSGSSN TDTGKVSGTL ETKYKWCEYG LTFTEKWNTD NTLGTEIAIE
110 120 130 140 150
DQICQGLKLT FDTTFSPNTG KKSGKIKSAY KRECINLGCD VDFDFAGPAI
160 170 180 190 200
HGSAVFGYEG WLAGYQMTFD SAKSKLTRSN FAVGYRTGDF QLHTNVNNGT
210 220 230 240 250
EFGGSIYQKV CEDFDTSVNL AWTSGTNCTR FGIAAKYQLD PTASISAKVN
260 270 280 290
NSSLIGVGYT QTLRPGVKLT LSALVDGKSF NAGGHKLGLA LELEA
Length:295
Mass (Da):31,746
Last modified:April 13, 2004 - v2
Checksum:i59582738AF84507F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121P → A AA sequence (PubMed:1373732).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039663 mRNA. Translation: BAB13474.1.
AF268468 mRNA. Translation: AAF80116.1.
BC063164 mRNA. Translation: AAH63164.1.
PIRiA38102.
RefSeqiNP_112644.1. NM_031354.1.
XP_006251723.1. XM_006251661.1.
UniGeneiRn.162037.

Genome annotation databases

EnsembliENSRNOT00000018462; ENSRNOP00000018462; ENSRNOG00000013505.
ENSRNOT00000086864; ENSRNOP00000074839; ENSRNOG00000013505.
GeneIDi83531.
KEGGirno:83531.
UCSCiRGD:621576. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039663 mRNA. Translation: BAB13474.1.
AF268468 mRNA. Translation: AAF80116.1.
BC063164 mRNA. Translation: AAH63164.1.
PIRiA38102.
RefSeqiNP_112644.1. NM_031354.1.
XP_006251723.1. XM_006251661.1.
UniGeneiRn.162037.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249747. 2 interactions.
IntActiP81155. 2 interactions.
MINTiMINT-4617801.
STRINGi10116.ENSRNOP00000018462.

PTM databases

iPTMnetiP81155.
PhosphoSiteiP81155.
SwissPalmiP81155.

2D gel databases

World-2DPAGE0004:P81155.

Proteomic databases

PaxDbiP81155.
PRIDEiP81155.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018462; ENSRNOP00000018462; ENSRNOG00000013505.
ENSRNOT00000086864; ENSRNOP00000074839; ENSRNOG00000013505.
GeneIDi83531.
KEGGirno:83531.
UCSCiRGD:621576. rat.

Organism-specific databases

CTDi7417.
RGDi621576. Vdac2.

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiP81155.
KOiK15040.
OMAiMCVPPSY.
OrthoDBiEOG7P8P8G.
PhylomeDBiP81155.

Miscellaneous databases

PROiP81155.

Gene expression databases

GenevisibleiP81155. RN.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030277. VDAC2.
[Graphical view]
PANTHERiPTHR11743:SF12. PTHR11743:SF12. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and cloning of a voltage-dependent anion channel-like Mr 36,000 polypeptide from mammalian brain."
    Bureau M.H., Khrestchatisky M., Heeren M.A., Zambrowicz E.B., Kim H., Grisar T.M., Colombini M., Tobin A.J., Olsen R.W.
    J. Biol. Chem. 267:8679-8684(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 63-96 AND 190-203.
    Tissue: Hippocampus.
  2. "Characterization of porin isoforms expressed in tumor cells."
    Shinohara Y., Ishida T., Hino M., Yamazaki N., Baba Y., Terada H.
    Eur. J. Biochem. 267:6067-6073(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Ascitic tumor.
  3. "Ion channels in the lens."
    Rae J.L.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Lens.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  5. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 47-121; 179-230 AND 237-278, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.
  6. "Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry."
    Distler A.M., Kerner J., Hoppel C.L.
    Biochim. Biophys. Acta 1774:628-636(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 236-243, PHOSPHORYLATION AT TYR-237, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Liver.
  7. "Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
    Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
    Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
  8. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVDAC2_RAT
AccessioniPrimary (citable) accession number: P81155
Secondary accession number(s): Q9JI32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 13, 2004
Last modified: July 6, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.