P81124 (GSTO_APLCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Probable glutathione transferase EC=2.5.1.18 Alternative name(s): Glutathione-dependent dehydroascorbate reductase EC=1.8.5.1 Monomethylarsonic acid reductase Short name=MMA(V) reductase EC=1.20.4.2 Protein 9
Organism	Aplysia californica (California sea hare)
Taxonomic identifier	6500 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Heterobranchia › Euthyneura › Euopisthobranchia › Aplysiomorpha › Aplysioidea › Aplysiidae › Aplysia

Protein attributes

Sequence length	92 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exhibits glutathione-dependent thiol transferase activity. Has dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione. UniProtKB P78417 2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate. Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.
Induction	By treatment with KCl and serotonin. Ref.1
Sequence similarities	Belongs to the GST superfamily. Omega family. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Oxidoreductase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	glutathione dehydrogenase (ascorbate) activity Inferred from electronic annotation. Source: EC glutathione transferase activity Inferred from electronic annotation. Source: EC methylarsonate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›92	›92	Probable glutathione transferase		PRO_0000291311
Regions
	Domain	‹1 – 71	›71	GST N-terminal
	Region	55 – 56	2	Glutathione binding By similarity
Sites
	Active site	3	1	Nucleophile By similarity UniProtKB P78417
	Binding site	30	1	Glutathione By similarity
	Binding site	43	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	92	1

Sequences

	Sequence		Length	Mass (Da)
	P81124 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F7C6B9ECA950CF14	FASTA	92	10,351
	10 20 30 40 50 60 RTCPYAQRAR LIIAAKGISA DLVNVDLNKK PDHFFDLNPY GEVPVVLHNG GHVYESLIAA 70 80 90 EYLEEAFPDP PLFAKEALVR ANERIYFNHA TK

References

[1]

"Effects on protein synthesis produced by pairing depolarization with serotonin, an analogue of associative learning in Aplysia." Noel F., Koumenis C., Nunez-Regueiro M., Raju U., Byrne J.H., Eskin A. Proc. Natl. Acad. Sci. U.S.A. 91:4150-4154(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-36 AND 56-92, NUCLEOTIDE SEQUENCE [MRNA] OF 37-55, INDUCTION. Tissue: Abdominal ganglion.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1EEM based on UniProtKB P78417.
ProteinModelPortal	P81124.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR004045. Glutathione_S-Trfase_N. IPR012336. Thioredoxin-like_fold. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTO_APLCA

Accession

Primary (citable) accession number: P81124

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)