ID   NUCB2_MOUSE             Reviewed;         420 AA.
AC   P81117;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2001, sequence version 2.
DT   21-SEP-2011, entry version 97.
DE   RecName: Full=Nucleobindin-2;
DE   AltName: Full=DNA-binding protein NEFA;
DE   Flags: Precursor;
GN   Name=Nucb2; Synonyms=Nefa;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RX   MEDLINE=20493539; PubMed=10915798; DOI=10.1074/jbc.M005103200;
RA   Taniguchi N., Taniura H., Niinobe M., Takayama C.,
RA   Tominaga-Yoshino K., Ogura A., Yoshikawa K.;
RT   "The postmitotic growth suppressor necdin interacts with a calcium-
RT   binding protein (NEFA) in neuronal cytoplasm.";
RL   J. Biol. Chem. 275:31674-31681(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Swiss Webster / NIH; TISSUE=Embryo;
RA   Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.;
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Calcium-binding protein. May have a role in calcium
CC       homeostasis.
CC   -!- SUBUNIT: Binds to the postmitotic growth suppressor necdin; co-
CC       expression abolishes Nucb2 secretion.
CC   -!- SUBCELLULAR LOCATION: Perikaryon. Endoplasmic reticulum. Nucleus
CC       envelope. Note=In dendrites and perikarya of brain neurons.
CC       Abundant in the ER cisternae and nuclear envelope, but not
CC       detected in Golgi, mitochondria or nucleoplasm. In cell culture,
CC       cytoplasmic and secreted.
CC   -!- TISSUE SPECIFICITY: Found in liver, heart, thymus, muscle,
CC       intestine, kidney, lung, spleen and throughout the brain, in
CC       cerebral cortex, hippocampus, hypothalamus and medulla oblongata.
CC       Nucb2 and necdin levels were higher in postmitotic neurons.
CC   -!- SIMILARITY: Belongs to the nucleobindin family.
CC   -!- SIMILARITY: Contains 2 EF-hand domains.
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DR   EMBL; AJ222586; CAA10858.1; -; mRNA.
DR   EMBL; BC010459; AAH10459.1; -; mRNA.
DR   IPI; IPI00309704; -.
DR   RefSeq; NP_001123951.1; NM_001130479.1.
DR   UniGene; Mm.9901; -.
DR   ProteinModelPortal; P81117; -.
DR   SMR; P81117; 229-327.
DR   STRING; P81117; -.
DR   PhosphoSite; P81117; -.
DR   PRIDE; P81117; -.
DR   Ensembl; ENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
DR   GeneID; 53322; -.
DR   KEGG; mmu:53322; -.
DR   UCSC; uc009jjk.2; mouse.
DR   CTD; 4925; -.
DR   MGI; MGI:1858179; Nucb2.
DR   eggNOG; roNOG09966; -.
DR   HOGENOM; HBG713576; -.
DR   HOVERGEN; HBG052685; -.
DR   InParanoid; P81117; -.
DR   OMA; DHPKVNH; -.
DR   OrthoDB; EOG4N04F6; -.
DR   PhylomeDB; P81117; -.
DR   ArrayExpress; P81117; -.
DR   Bgee; P81117; -.
DR   CleanEx; MM_NUCB2; -.
DR   Genevestigator; P81117; -.
DR   GermOnline; ENSMUSG00000030659; Mus musculus.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:MGI.
DR   GO; GO:0005640; C:nuclear outer membrane; IDA:MGI.
DR   GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006874; P:cellular calcium ion homeostasis; TAS:MGI.
DR   InterPro; IPR011992; EF-hand-like_dom.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR018249; EF_HAND_2.
DR   InterPro; IPR002048; EF_hand_Ca-bd.
DR   Gene3D; G3DSA:1.10.238.10; EF-Hand_type; 1.
DR   SMART; SM00054; EFh; 2.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   Calcium; Complete proteome; DNA-binding; Endoplasmic reticulum;
KW   Nucleus; Reference proteome; Repeat; Signal.
FT   SIGNAL        1     24       Potential.
FT   CHAIN        25    420       Nucleobindin-2.
FT                                /FTId=PRO_0000004166.
FT   DOMAIN      241    276       EF-hand 1.
FT   DOMAIN      293    328       EF-hand 2.
FT   DNA_BIND    171    223       By similarity.
FT   CA_BIND     254    265       1.
FT   CA_BIND     306    317       2.
FT   REGION      213    420       Binds to necdin.
FT   COMPBIAS    284    293       Asp/Glu-rich (acidic).
FT   CONFLICT     18     18       M -> T (in Ref. 2; CAA10858).
SQ   SEQUENCE   420 AA;  50304 MW;  0DEBD51724CB7E72 CRC64;
     MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT GLYYDEYLKQ
     VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA
     KLDALQDTGM NHHLLLKQFE HLNHQNPNTF ESRDLDMLIK AATADLEQYD RTRHEEFKKY
     EMMKEHERRE YLKTLSEEKR KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD
     PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH
     VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY ESIIAIQENE
     LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK LQQGIAPSGP AGELKFEPHT
//