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P81117 (NUCB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleobindin-2
Alternative name(s):
DNA-binding protein NEFA
Prepronesfatin

Cleaved into the following chain:

  1. Nesfatin-1
Gene names
Name:Nucb2
Synonyms:Nefa
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-binding protein. May have a role in calcium homeostasis. Ref.1

Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance By similarity. Ref.1

Subunit structure

Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion. Ref.1

Subcellular location

Cytoplasm. Perikaryon. Endoplasmic reticulum. Golgi apparatus By similarity. Nucleus envelope. Membrane; Peripheral membrane protein By similarity. Secreted. Note: In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted. Ref.1

Nesfatin-1: Secreted By similarity Ref.1.

Tissue specificity

Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons. Ref.1

Sequence similarities

Belongs to the nucleobindin family.

Contains 2 EF-hand domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 420396Nucleobindin-2
PRO_0000004166
Chain25 – 10682Nesfatin-1
PRO_0000419820

Regions

Domain241 – 27636EF-hand 1
Domain293 – 32836EF-hand 2
DNA binding171 – 22353 By similarity
Calcium binding254 – 265121
Calcium binding306 – 317122
Region213 – 420208Binds to necdin
Compositional bias284 – 29310Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue3321Phosphoserine By similarity

Experimental info

Sequence conflict181M → T in CAA10858. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P81117 [UniParc].

Last modified November 2, 2001. Version 2.
Checksum: 0DEBD51724CB7E72

FASTA42050,304
        10         20         30         40         50         60 
MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT GLYYDEYLKQ 

        70         80         90        100        110        120 
VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA 

       130        140        150        160        170        180 
KLDALQDTGM NHHLLLKQFE HLNHQNPNTF ESRDLDMLIK AATADLEQYD RTRHEEFKKY 

       190        200        210        220        230        240 
EMMKEHERRE YLKTLSEEKR KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD 

       250        260        270        280        290        300 
PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH 

       310        320        330        340        350        360 
VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY ESIIAIQENE 

       370        380        390        400        410        420 
LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK LQQGIAPSGP AGELKFEPHT 

« Hide

References

« Hide 'large scale' references
[1]"The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm."
Taniguchi N., Taniura H., Niinobe M., Takayama C., Tominaga-Yoshino K., Ogura A., Yoshikawa K.
J. Biol. Chem. 275:31674-31681(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"cDNA sequence analysis of mouse NEFA gene."
Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster / NIH.
Tissue: Embryo.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ222586 mRNA. Translation: CAA10858.1.
BC010459 mRNA. Translation: AAH10459.1.
RefSeqNP_001123951.1. NM_001130479.2.
XP_006508087.1. XM_006508024.1.
XP_006508088.1. XM_006508025.1.
UniGeneMm.9901.

3D structure databases

ProteinModelPortalP81117.
SMRP81117. Positions 229-327.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP81117. 1 interaction.
MINTMINT-4104248.

PTM databases

PhosphoSiteP81117.

Proteomic databases

PaxDbP81117.
PRIDEP81117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
GeneID53322.
KEGGmmu:53322.
UCSCuc009jjk.2. mouse.

Organism-specific databases

CTD4925.
MGIMGI:1858179. Nucb2.

Phylogenomic databases

eggNOGNOG262100.
GeneTreeENSGT00390000001927.
HOGENOMHOG000007216.
HOVERGENHBG052685.
InParanoidP81117.
OMADHPKVNH.
OrthoDBEOG7WQ7SZ.
PhylomeDBP81117.
TreeFamTF323218.

Gene expression databases

ArrayExpressP81117.
BgeeP81117.
CleanExMM_NUCB2.
GenevestigatorP81117.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view]
PANTHERPTHR19237:SF3. PTHR19237:SF3. 1 hit.
PfamPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNUCB2. mouse.
NextBio310129.
PROP81117.
SOURCESearch...

Entry information

Entry nameNUCB2_MOUSE
AccessionPrimary (citable) accession number: P81117
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 2, 2001
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot