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P81117

- NUCB2_MOUSE

UniProt

P81117 - NUCB2_MOUSE

Protein

Nucleobindin-2

Gene

Nucb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (02 Nov 2001)
      Previous versions | rss
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    Functioni

    Calcium-binding protein. May have a role in calcium homeostasis.1 Publication
    Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi171 – 22353By similarityAdd
    BLAST
    Calcium bindingi254 – 265121Add
    BLAST
    Calcium bindingi306 – 317122Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: MGI
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular calcium ion homeostasis Source: MGI

    Keywords - Ligandi

    Calcium, DNA-binding, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleobindin-2
    Alternative name(s):
    DNA-binding protein NEFA
    Prepronesfatin
    Cleaved into the following chain:
    Gene namesi
    Name:Nucb2
    Synonyms:Nefa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1858179. Nucb2.

    Subcellular locationi

    Cytoplasm 1 Publication. Perikaryon 1 Publication. Endoplasmic reticulum 1 Publication. Golgi apparatus By similarity. Nucleus envelope 1 Publication. Membrane By similarity; Peripheral membrane protein By similarity. Secreted 1 Publication
    Note: In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted.
    Chain Nesfatin-1 : Secreted By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. endoplasmic reticulum Source: MGI
    3. endoplasmic reticulum-Golgi intermediate compartment Source: Ensembl
    4. extracellular region Source: UniProtKB-SubCell
    5. Golgi apparatus Source: UniProtKB-SubCell
    6. nuclear outer membrane Source: MGI
    7. nucleus Source: MGI
    8. perikaryon Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 420396Nucleobindin-2PRO_0000004166Add
    BLAST
    Chaini25 – 10682Nesfatin-1PRO_0000419820Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei332 – 3321PhosphoserineBy similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Phosphoprotein

    Proteomic databases

    MaxQBiP81117.
    PaxDbiP81117.
    PRIDEiP81117.

    PTM databases

    PhosphoSiteiP81117.

    Expressioni

    Tissue specificityi

    Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.1 Publication

    Gene expression databases

    ArrayExpressiP81117.
    BgeeiP81117.
    CleanExiMM_NUCB2.
    GenevestigatoriP81117.

    Interactioni

    Subunit structurei

    Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion.1 Publication

    Protein-protein interaction databases

    IntActiP81117. 1 interaction.
    MINTiMINT-4104248.

    Structurei

    3D structure databases

    ProteinModelPortaliP81117.
    SMRiP81117. Positions 229-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini241 – 27636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini293 – 32836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni213 – 420208Binds to necdinAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi284 – 29310Asp/Glu-rich (acidic)

    Sequence similaritiesi

    Belongs to the nucleobindin family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG262100.
    GeneTreeiENSGT00390000001927.
    HOGENOMiHOG000007216.
    HOVERGENiHBG052685.
    InParanoidiP81117.
    OrthoDBiEOG7WQ7SZ.
    PhylomeDBiP81117.
    TreeFamiTF323218.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028813. NUCB2.
    [Graphical view]
    PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
    PfamiPF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P81117-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT    50
    GLYYDEYLKQ VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR 100
    TRLDELKRQE VGRLRMLIKA KLDALQDTGM NHHLLLKQFE HLNHQNPNTF 150
    ESRDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLSEEKR 200
    KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF 250
    KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH 300
    VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY 350
    ESIIAIQENE LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK 400
    LQQGIAPSGP AGELKFEPHT 420
    Length:420
    Mass (Da):50,304
    Last modified:November 2, 2001 - v2
    Checksum:i0DEBD51724CB7E72
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 181M → T in CAA10858. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ222586 mRNA. Translation: CAA10858.1.
    BC010459 mRNA. Translation: AAH10459.1.
    CCDSiCCDS52372.1.
    RefSeqiNP_001123951.1. NM_001130479.2.
    XP_006508087.1. XM_006508024.1.
    XP_006508088.1. XM_006508025.1.
    UniGeneiMm.9901.

    Genome annotation databases

    EnsembliENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
    GeneIDi53322.
    KEGGimmu:53322.
    UCSCiuc009jjk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ222586 mRNA. Translation: CAA10858.1 .
    BC010459 mRNA. Translation: AAH10459.1 .
    CCDSi CCDS52372.1.
    RefSeqi NP_001123951.1. NM_001130479.2.
    XP_006508087.1. XM_006508024.1.
    XP_006508088.1. XM_006508025.1.
    UniGenei Mm.9901.

    3D structure databases

    ProteinModelPortali P81117.
    SMRi P81117. Positions 229-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P81117. 1 interaction.
    MINTi MINT-4104248.

    PTM databases

    PhosphoSitei P81117.

    Proteomic databases

    MaxQBi P81117.
    PaxDbi P81117.
    PRIDEi P81117.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032895 ; ENSMUSP00000032895 ; ENSMUSG00000030659 .
    GeneIDi 53322.
    KEGGi mmu:53322.
    UCSCi uc009jjk.2. mouse.

    Organism-specific databases

    CTDi 4925.
    MGIi MGI:1858179. Nucb2.

    Phylogenomic databases

    eggNOGi NOG262100.
    GeneTreei ENSGT00390000001927.
    HOGENOMi HOG000007216.
    HOVERGENi HBG052685.
    InParanoidi P81117.
    OrthoDBi EOG7WQ7SZ.
    PhylomeDBi P81117.
    TreeFami TF323218.

    Miscellaneous databases

    ChiTaRSi NUCB2. mouse.
    NextBioi 310129.
    PROi P81117.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P81117.
    Bgeei P81117.
    CleanExi MM_NUCB2.
    Genevestigatori P81117.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028813. NUCB2.
    [Graphical view ]
    PANTHERi PTHR19237:SF22. PTHR19237:SF22. 1 hit.
    Pfami PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm."
      Taniguchi N., Taniura H., Niinobe M., Takayama C., Tominaga-Yoshino K., Ogura A., Yoshikawa K.
      J. Biol. Chem. 275:31674-31681(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "cDNA sequence analysis of mouse NEFA gene."
      Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss Webster / NIH.
      Tissue: Embryo.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiNUCB2_MOUSE
    AccessioniPrimary (citable) accession number: P81117
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 2, 2001
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3