P81117 (NUCB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleobindin-2

Alternative name(s):
DNA-binding protein NEFA

Gene names

Name:	Nucb2
Synonyms:	Nefa

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-binding protein. May have a role in calcium homeostasis.
Subunit structure	Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion.
Subcellular location	Perikaryon. Endoplasmic reticulum. Nucleus envelope. Note: In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm. In cell culture, cytoplasmic and secreted.
Tissue specificity	Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.
Sequence similarities	Belongs to the nucleobindin family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Nucleus
Domain	Repeat Signal
Ligand	Calcium DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular calcium ion homeostasis Traceable author statement Ref.1. Source: MGI
Cellular component	endoplasmic reticulum Inferred from direct assay Ref.1. Source: MGI nuclear outer membrane Inferred from direct assay Ref.1. Source: MGI perikaryon Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 420	396	Nucleobindin-2		PRO_0000004166
Regions
	Domain	241 – 276	36	EF-hand 1
	Domain	293 – 328	36	EF-hand 2
	DNA binding	171 – 223	53	By similarity
	Calcium binding	254 – 265	12	1
	Calcium binding	306 – 317	12	2
	Region	213 – 420	208	Binds to necdin
	Compositional bias	284 – 293	10	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	18	1	M → T in CAA10858. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P81117 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 0DEBD51724CB7E72	FASTA	420	50,304
	10 20 30 40 50 60 MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT GLYYDEYLKQ 70 80 90 100 110 120 VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA 130 140 150 160 170 180 KLDALQDTGM NHHLLLKQFE HLNHQNPNTF ESRDLDMLIK AATADLEQYD RTRHEEFKKY 190 200 210 220 230 240 EMMKEHERRE YLKTLSEEKR KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD 250 260 270 280 290 300 PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH 310 320 330 340 350 360 VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY ESIIAIQENE 370 380 390 400 410 420 LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK LQQGIAPSGP AGELKFEPHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm." Taniguchi N., Taniura H., Niinobe M., Takayama C., Tominaga-Yoshino K., Ogura A., Yoshikawa K. J. Biol. Chem. 275:31674-31681(2000) [PubMed: 10915798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster / NIH. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ222586 mRNA. Translation: CAA10858.1. BC010459 mRNA. Translation: AAH10459.1.
IPI	IPI00309704.
RefSeq	NP_001123951.1. NM_001130479.1.
UniGene	Mm.9901.
3D structure databases
ProteinModelPortal	P81117.
SMR	P81117. Positions 229-327.
ModBase	Search...
Protein-protein interaction databases
STRING	P81117.
PTM databases
PhosphoSite	P81117.
Proteomic databases
PRIDE	P81117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
GeneID	53322.
KEGG	mmu:53322.
UCSC	uc009jjk.2. mouse.
Organism-specific databases
CTD	4925.
MGI	MGI:1858179. Nucb2.
Phylogenomic databases
eggNOG	roNOG09966.
HOGENOM	HBG713576.
HOVERGEN	HBG052685.
InParanoid	P81117.
OMA	DHPKVNH.
OrthoDB	EOG4N04F6.
PhylomeDB	P81117.
Gene expression databases
ArrayExpress	P81117.
Bgee	P81117.
CleanEx	MM_NUCB2.
Genevestigator	P81117.
GermOnline	ENSMUSG00000030659. Mus musculus.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

NUCB2_MOUSE

Accession

Primary (citable) accession number: P81117

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 2, 2001
Last modified:	September 21, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program