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Protein

Nucleobindin-2

Gene

Nucb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein. May have a role in calcium homeostasis.1 Publication
Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi171 – 22353By similarityAdd
BLAST
Calcium bindingi254 – 265121Add
BLAST
Calcium bindingi306 – 317122Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: MGI
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular calcium ion homeostasis Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-2
Alternative name(s):
DNA-binding protein NEFA
Prepronesfatin
Cleaved into the following chain:
Gene namesi
Name:Nucb2
Synonyms:Nefa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1858179. Nucb2.

Subcellular locationi

Cytoplasm 1 Publication. Perikaryon 1 Publication. Endoplasmic reticulum 1 Publication. Golgi apparatus By similarity. Nucleus envelope 1 Publication. Membrane By similarity; Peripheral membrane protein By similarity. Secreted 1 Publication
Note: In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted.
Chain Nesfatin-1 : Secreted By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. endoplasmic reticulum Source: MGI
  3. endoplasmic reticulum-Golgi intermediate compartment Source: MGI
  4. extracellular space Source: MGI
  5. extracellular vesicular exosome Source: MGI
  6. Golgi apparatus Source: MGI
  7. nuclear outer membrane Source: MGI
  8. nucleus Source: MGI
  9. perikaryon Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 420396Nucleobindin-2PRO_0000004166Add
BLAST
Chaini25 – 10682Nesfatin-1PRO_0000419820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei332 – 3321PhosphoserineBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Phosphoprotein

Proteomic databases

MaxQBiP81117.
PaxDbiP81117.
PRIDEiP81117.

PTM databases

PhosphoSiteiP81117.

Expressioni

Tissue specificityi

Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.1 Publication

Gene expression databases

BgeeiP81117.
CleanExiMM_NUCB2.
ExpressionAtlasiP81117. baseline and differential.
GenevestigatoriP81117.

Interactioni

Subunit structurei

Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion.1 Publication

Protein-protein interaction databases

IntActiP81117. 1 interaction.
MINTiMINT-4104248.

Structurei

3D structure databases

ProteinModelPortaliP81117.
SMRiP81117. Positions 229-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini241 – 27636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini293 – 32836EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni213 – 420208Binds to necdinAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi284 – 29310Asp/Glu-rich (acidic)

Sequence similaritiesi

Belongs to the nucleobindin family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG262100.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiP81117.
OrthoDBiEOG7WQ7SZ.
PhylomeDBiP81117.
TreeFamiTF323218.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view]
PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81117-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT
60 70 80 90 100
GLYYDEYLKQ VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR
110 120 130 140 150
TRLDELKRQE VGRLRMLIKA KLDALQDTGM NHHLLLKQFE HLNHQNPNTF
160 170 180 190 200
ESRDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLSEEKR
210 220 230 240 250
KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF
260 270 280 290 300
KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH
310 320 330 340 350
VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY
360 370 380 390 400
ESIIAIQENE LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK
410 420
LQQGIAPSGP AGELKFEPHT
Length:420
Mass (Da):50,304
Last modified:November 2, 2001 - v2
Checksum:i0DEBD51724CB7E72
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181M → T in CAA10858 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222586 mRNA. Translation: CAA10858.1.
BC010459 mRNA. Translation: AAH10459.1.
CCDSiCCDS52372.1.
RefSeqiNP_001123951.1. NM_001130479.2.
XP_006508087.1. XM_006508024.2.
XP_006508088.1. XM_006508025.2.
UniGeneiMm.9901.

Genome annotation databases

EnsembliENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
GeneIDi53322.
KEGGimmu:53322.
UCSCiuc009jjk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222586 mRNA. Translation: CAA10858.1.
BC010459 mRNA. Translation: AAH10459.1.
CCDSiCCDS52372.1.
RefSeqiNP_001123951.1. NM_001130479.2.
XP_006508087.1. XM_006508024.2.
XP_006508088.1. XM_006508025.2.
UniGeneiMm.9901.

3D structure databases

ProteinModelPortaliP81117.
SMRiP81117. Positions 229-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP81117. 1 interaction.
MINTiMINT-4104248.

PTM databases

PhosphoSiteiP81117.

Proteomic databases

MaxQBiP81117.
PaxDbiP81117.
PRIDEiP81117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
GeneIDi53322.
KEGGimmu:53322.
UCSCiuc009jjk.2. mouse.

Organism-specific databases

CTDi4925.
MGIiMGI:1858179. Nucb2.

Phylogenomic databases

eggNOGiNOG262100.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiP81117.
OrthoDBiEOG7WQ7SZ.
PhylomeDBiP81117.
TreeFamiTF323218.

Miscellaneous databases

ChiTaRSiNucb2. mouse.
NextBioi310129.
PROiP81117.
SOURCEiSearch...

Gene expression databases

BgeeiP81117.
CleanExiMM_NUCB2.
ExpressionAtlasiP81117. baseline and differential.
GenevestigatoriP81117.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view]
PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm."
    Taniguchi N., Taniura H., Niinobe M., Takayama C., Tominaga-Yoshino K., Ogura A., Yoshikawa K.
    J. Biol. Chem. 275:31674-31681(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "cDNA sequence analysis of mouse NEFA gene."
    Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster / NIH.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiNUCB2_MOUSE
AccessioniPrimary (citable) accession number: P81117
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 2, 2001
Last modified: April 1, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.