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Protein

Nucleobindin-2

Gene

Nucb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein. May have a role in calcium homeostasis.1 Publication
Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi171 – 223By similarityAdd BLAST53
Calcium bindingi254 – 2651Add BLAST12
Calcium bindingi306 – 3172Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • cellular calcium ion homeostasis Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-2
Alternative name(s):
DNA-binding protein NEFA
Prepronesfatin
Cleaved into the following chain:
Gene namesi
Name:Nucb2
Synonyms:Nefa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1858179. Nucb2.

Subcellular locationi

Nesfatin-1 :

GO - Cellular componenti

  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum-Golgi intermediate compartment Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • Golgi apparatus Source: MGI
  • nuclear outer membrane Source: MGI
  • nucleus Source: MGI
  • perikaryon Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000416625 – 420Nucleobindin-2Add BLAST396
ChainiPRO_000041982025 – 106Nesfatin-1Add BLAST82

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei332PhosphoserineBy similarity1

Keywords - PTMi

Cleavage on pair of basic residues, Phosphoprotein

Proteomic databases

EPDiP81117.
MaxQBiP81117.
PaxDbiP81117.
PeptideAtlasiP81117.
PRIDEiP81117.

PTM databases

iPTMnetiP81117.
PhosphoSitePlusiP81117.

Expressioni

Tissue specificityi

Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.1 Publication

Gene expression databases

BgeeiENSMUSG00000030659.
CleanExiMM_NUCB2.
ExpressionAtlasiP81117. baseline and differential.
GenevisibleiP81117. MM.

Interactioni

Subunit structurei

Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion.1 Publication

Protein-protein interaction databases

IntActiP81117. 1 interactor.
MINTiMINT-4104248.
STRINGi10090.ENSMUSP00000032895.

Structurei

3D structure databases

ProteinModelPortaliP81117.
SMRiP81117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini241 – 276EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini293 – 328EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 420Binds to necdinAdd BLAST208

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi284 – 293Asp/Glu-rich (acidic)10

Sequence similaritiesi

Belongs to the nucleobindin family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiP81117.
KOiK20371.
PhylomeDBiP81117.
TreeFamiTF323218.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view]
PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81117-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT
60 70 80 90 100
GLYYDEYLKQ VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR
110 120 130 140 150
TRLDELKRQE VGRLRMLIKA KLDALQDTGM NHHLLLKQFE HLNHQNPNTF
160 170 180 190 200
ESRDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLSEEKR
210 220 230 240 250
KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF
260 270 280 290 300
KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH
310 320 330 340 350
VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY
360 370 380 390 400
ESIIAIQENE LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK
410 420
LQQGIAPSGP AGELKFEPHT
Length:420
Mass (Da):50,304
Last modified:November 2, 2001 - v2
Checksum:i0DEBD51724CB7E72
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18M → T in CAA10858 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222586 mRNA. Translation: CAA10858.1.
BC010459 mRNA. Translation: AAH10459.1.
CCDSiCCDS52372.1.
RefSeqiNP_001123951.1. NM_001130479.2.
XP_006508087.1. XM_006508024.2.
XP_006508088.1. XM_006508025.2.
UniGeneiMm.9901.

Genome annotation databases

EnsembliENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
GeneIDi53322.
KEGGimmu:53322.
UCSCiuc009jjk.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222586 mRNA. Translation: CAA10858.1.
BC010459 mRNA. Translation: AAH10459.1.
CCDSiCCDS52372.1.
RefSeqiNP_001123951.1. NM_001130479.2.
XP_006508087.1. XM_006508024.2.
XP_006508088.1. XM_006508025.2.
UniGeneiMm.9901.

3D structure databases

ProteinModelPortaliP81117.
SMRiP81117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP81117. 1 interactor.
MINTiMINT-4104248.
STRINGi10090.ENSMUSP00000032895.

PTM databases

iPTMnetiP81117.
PhosphoSitePlusiP81117.

Proteomic databases

EPDiP81117.
MaxQBiP81117.
PaxDbiP81117.
PeptideAtlasiP81117.
PRIDEiP81117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
GeneIDi53322.
KEGGimmu:53322.
UCSCiuc009jjk.3. mouse.

Organism-specific databases

CTDi4925.
MGIiMGI:1858179. Nucb2.

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiP81117.
KOiK20371.
PhylomeDBiP81117.
TreeFamiTF323218.

Miscellaneous databases

ChiTaRSiNucb2. mouse.
PROiP81117.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030659.
CleanExiMM_NUCB2.
ExpressionAtlasiP81117. baseline and differential.
GenevisibleiP81117. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view]
PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUCB2_MOUSE
AccessioniPrimary (citable) accession number: P81117
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 2, 2001
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.