Nucleobindin-2 precursor - Mus musculus (Mouse)

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P81117 (NUCB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nucleobindin-2

Alternative name(s):
DNA-binding protein NEFA
Prepronesfatin

Cleaved into the following chain:

Nesfatin-1

Gene names

Name:	Nucb2
Synonyms:	Nefa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-binding protein. May have a role in calcium homeostasis. Ref.1 Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance By similarity. Ref.1
Subunit structure	Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion. Ref.1
Subcellular location	Cytoplasm. Perikaryon. Endoplasmic reticulum. Golgi apparatus By similarity. Nucleus envelope. Membrane; Peripheral membrane protein By similarity. Secreted. Note: In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted. Ref.1 Nesfatin-1: Secreted By similarity Ref.1.
Tissue specificity	Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons. Ref.1
Sequence similarities	Belongs to the nucleobindin family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Golgi apparatus Membrane Nucleus Secreted
Domain	Repeat Signal
Ligand	Calcium DNA-binding Metal-binding
PTM	Cleavage on pair of basic residues Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular calcium ion homeostasis Traceable author statement Ref.1. Source: MGI
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay Ref.1. Source: MGI endoplasmic reticulum-Golgi intermediate compartment Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear outer membrane Inferred from direct assay Ref.1. Source: MGI perikaryon Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

Potential

Chain

25 – 420

396

Nucleobindin-2

PRO_0000004166

Chain

25 – 106

Nesfatin-1

PRO_0000419820

Regions

Domain

241 – 276

EF-hand 1

Domain

293 – 328

EF-hand 2

DNA binding

171 – 223

By similarity

Calcium binding

254 – 265

Calcium binding

306 – 317

Region

213 – 420

208

Binds to necdin

Compositional bias

284 – 293

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

332

Phosphoserine By similarity

Experimental info

Sequence conflict

M → T in CAA10858. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P81117 [UniParc].

Last modified November 2, 2001. Version 2.
Checksum: 0DEBD51724CB7E72
Show »

FASTA

420

50,304

        10         20         30         40         50         60 
MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT GLYYDEYLKQ 

        70         80         90        100        110        120 
VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA 

       130        140        150        160        170        180 
KLDALQDTGM NHHLLLKQFE HLNHQNPNTF ESRDLDMLIK AATADLEQYD RTRHEEFKKY 

       190        200        210        220        230        240 
EMMKEHERRE YLKTLSEEKR KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD 

       250        260        270        280        290        300 
PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH 

       310        320        330        340        350        360 
VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY ESIIAIQENE 

       370        380        390        400        410        420 
LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK LQQGIAPSGP AGELKFEPHT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm." Taniguchi N., Taniura H., Niinobe M., Takayama C., Tominaga-Yoshino K., Ogura A., Yoshikawa K. J. Biol. Chem. 275:31674-31681(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"cDNA sequence analysis of mouse NEFA gene." Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster / NIH. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ222586 mRNA. Translation: CAA10858.1. BC010459 mRNA. Translation: AAH10459.1.
IPI	IPI00309704.
RefSeq	NP_001123951.1. NM_001130479.2.
UniGene	Mm.9901.
3D structure databases
ProteinModelPortal	P81117.
SMR	P81117. Positions 229-327.
ModBase	Search...
PTM databases
PhosphoSite	P81117.
Proteomic databases
PaxDb	P81117.
PRIDE	P81117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032895; ENSMUSP00000032895; ENSMUSG00000030659.
GeneID	53322.
KEGG	mmu:53322.
UCSC	uc009jjk.2. mouse.
Organism-specific databases
CTD	4925.
MGI	MGI:1858179. Nucb2.
Phylogenomic databases
eggNOG	NOG262100.
GeneTree	ENSGT00390000001927.
HOGENOM	HOG000007216.
HOVERGEN	HBG052685.
InParanoid	P81117.
OMA	DHPKVNH.
OrthoDB	EOG4N04F6.
Gene expression databases
ArrayExpress	P81117.
Bgee	P81117.
CleanEx	MM_NUCB2.
Genevestigator	P81117.
GermOnline	ENSMUSG00000030659. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NUCB2. mouse.
NextBio	310129.
SOURCE	Search...

Entry information

Entry name

NUCB2_MOUSE

Accession

Primary (citable) accession number: P81117

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 2, 2001
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents