Reviewed, UniProtKB/Swiss-Prot P81117 (NUCB2_MOUSE)

Names and origin

Protein names

Recommended name:
    Nucleobindin-2
Alternative name(s):
    DNA-binding protein NEFA

Gene names

Name:	Nucb2
Synonyms:	Nefa

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Calcium-binding protein. May have a role in calcium homeostasis.
Subunit structure	Binds to the postmitotic growth suppressor necdin; co-expression abolishes Nucb2 secretion.
Subcellular location	Perikaryon. Endoplasmic reticulum. Nucleus envelope. Note= In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm. In cell culture, cytoplasmic and secreted.
Tissue specificity	Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.
Sequence similarities	Belongs to the nucleobindin family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Nucleus
Domain	Repeat Signal
Ligand	Calcium DNA-binding
Gene Ontology (GO)
Biological process	cellular calcium ion homeostasis Ref.1 Traceable author statement. Source: MGI
Cellular component	endoplasmic reticulum Ref.1 Inferred from direct assay. Source: MGI nuclear outer membrane Ref.1 Inferred from direct assay. Source: MGI perikaryon Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 420	396	Nucleobindin-2		PRO_0000004166
Regions
	Domain	241 – 276	36	EF-hand 1
	Domain	293 – 328	36	EF-hand 2
	DNA binding	171 – 223	53	By similarity
	Calcium binding	254 – 265	12	1
	Calcium binding	306 – 317	12	2
	Region	213 – 420	208	Binds to necdin
	Compositional bias	284 – 293	10	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	18	1	M → T in CAA10858. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P81117-1 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 0DEBD51724CB7E72	FASTA	420	50,304
	10 20 30 40 50 60 MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT GLYYDEYLKQ 70 80 90 100 110 120 VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA 130 140 150 160 170 180 KLDALQDTGM NHHLLLKQFE HLNHQNPNTF ESRDLDMLIK AATADLEQYD RTRHEEFKKY 190 200 210 220 230 240 EMMKEHERRE YLKTLSEEKR KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD 250 260 270 280 290 300 PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH 310 320 330 340 350 360 VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY ESIIAIQENE 370 380 390 400 410 420 LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK LQQGIAPSGP AGELKFEPHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm." Taniguchi N., Taniura H., Niinobe M., Takayama C., Tominaga-Yoshino K., Ogura A., Yoshikawa K. J. Biol. Chem. 275:31674-31681(2000) [PubMed: 10915798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	Hoefig K., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster / NIH. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ222586 mRNA. Translation: CAA10858.1. BC010459 mRNA. Translation: AAH10459.1.
RefSeq	NP_001123951.1.
UniGene	Mm.9901
3D structure databases
SMR	P81117. Positions 229-327.
ModBase	Search...
PTM databases
PhosphoSite	P81117.
Genome annotation databases
Ensembl	ENSMUSG00000030659. Mus musculus. [Contig view]
GeneID	53322.
Organism-specific databases
MGI	MGI:1858179. Nucb2.
Phylogenomic databases
HOGENOM	P81117.
HOVERGEN	P81117.
Gene expression databases
ArrayExpress	P81117.
CleanEx	MM_NUCB2.
GermOnline	ENSMUSG00000030659. Mus musculus.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR002048. EF_hand_Ca_bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 2 hits. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

NUCB2_MOUSE

Accession

Primary (citable) accession number: P81117

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 2, 2001
Last modified:	November 25, 2008
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)