P81076 (AIF1_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Allograft inflammatory factor 1
Short name=AIF-1

Alternative name(s):
Daintain

Gene names

Name:

AIF1

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation By similarity. Has a dual influence on glucose-induced insulin secretion: inhibition at low concentration and stimulation at high concentrations.
Subunit structure	Homodimer Potential. Monomer. Interacts with LCP1 By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis By similarity.
Tissue specificity	Microglial cells in the central nervous system and dendritic cells and macrophages in several organs.
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane
Domain	Repeat
Ligand	Actin-binding Calcium Metal-binding
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Rac protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB actin filament bundle assembly Inferred from sequence or structural similarity. Source: UniProtKB actin filament polymerization Inferred from sequence or structural similarity. Source: UniProtKB cellular response to interferon-gamma Inferred from sequence or structural similarity. Source: UniProtKB inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB phagocytosis, engulfment Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of G1/S transition of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of T cell migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of T cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of monocyte chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of smooth muscle cell chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of smooth muscle cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB ruffle assembly Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from sequence or structural similarity. Source: UniProtKB lamellipodium Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB phagocytic cup Inferred from sequence or structural similarity. Source: UniProtKB ruffle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	actin filament binding Inferred from sequence or structural similarity. Source: UniProtKB calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 146	146	Allograft inflammatory factor 1		PRO_0000073868
Regions
	Domain	44 – 79	36	EF-hand 1
	Domain	81 – 115	35	EF-hand 2; degenerate
	Calcium binding	57 – 68	12	1 Potential
	Calcium binding	93 – 104	12	2 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylserine
	Modified residue	10	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81076 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 018FD1D8698E86EF	FASTA	146	16,560
	10 20 30 40 50 60 SETIDLQGGK AFGLLKAQQE GRLNEINKQF LDDPKYSSDE DLSRKLEAFK QKYMEFDLNG 70 80 90 100 110 120 NGDIDIMSLK RMLEKLGVPK THLELKKLIK EVSSGSGETF SYSIFLKMML GKRSAILKMI 130 140 LMYEEKAREQ EKPTGPPAKK AISELP

References

[1]

"Identification, isolation, and characterization of daintain (allograft inflammatory factor 1), a macrophage polypeptide with effects on insulin secretion and abundantly present in the pancreas of prediabetic BB rats." Chen Z.-W., Ahren B., Oestenson C.-G., Cintra A., Bergman T., Moeller C., Fuxe K., Mutt V., Joernvall H., Efendic S. Proc. Natl. Acad. Sci. U.S.A. 94:13879-13884(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Intestine.

Cross-references

3D structure databases
ProteinModelPortal	P81076.
SMR	P81076. Positions 1-146.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG262998.
HOVERGEN	HBG004002.
OrthoDB	EOG41C6XK.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR002048. EF_hand_dom. [Graphical view]
PROSITE	PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AIF1_PIG

Accession

Primary (citable) accession number: P81076

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program