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P81068

- IRX1_MOUSE

UniProt

P81068 - IRX1_MOUSE

Protein

Iroquois-class homeodomain protein IRX-1

Gene

Irx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 4 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi127 – 18963Homeobox; TALE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. metanephros development Source: UniProtKB
    2. proximal/distal pattern formation involved in metanephric nephron development Source: UniProtKB
    3. specification of loop of Henle identity Source: UniProtKB

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iroquois-class homeodomain protein IRX-1
    Alternative name(s):
    Homeodomain protein IRXA1
    Iroquois homeobox protein 1
    Gene namesi
    Name:Irx1
    Synonyms:Irxa1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1197515. Irx1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 480480Iroquois-class homeodomain protein IRX-1PRO_0000049152Add
    BLAST

    Proteomic databases

    PRIDEiP81068.

    PTM databases

    PhosphoSiteiP81068.

    Expressioni

    Tissue specificityi

    Expressed in specific and overlapping patterns with Irx1 and Irx2 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule.1 Publication

    Developmental stagei

    First detected in the mesoderm at stage E7.5. During neurogenesis (E9.5 to E10.5), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon, rhombencephalon and spinal cord, with expression excluded from the midbrain-hindbrain junction. Beginning at E9.5, expression is found in the epithelial component of the branchial arches and foregut. At later stages, expressed in somites. At E10.5, expressed in the cephalic mesenchyme surrounding the optic vesicle. By E12.5, expression remains in the mesenchyme and also begins in the neuroretina, and at E16.5 expression is exclusively located in the inner neuroblast layers of the neuroretina. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from E10.5 onwards. Expressed in distinct patterns in the developing limb buds from E10.5 onwards. Expressed in the developing heart in the ventricular septum from the onset of its formation (E10.5) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system.3 Publications

    Gene expression databases

    CleanExiMM_IRX1.
    GenevestigatoriP81068.

    Structurei

    3D structure databases

    ProteinModelPortaliP81068.
    SMRiP81068. Positions 136-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi29 – 3810Poly-Ala
    Compositional biasi334 – 3374Poly-Pro
    Compositional biasi409 – 4157Poly-Pro

    Sequence similaritiesi

    Belongs to the TALE/IRO homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG279452.
    GeneTreeiENSGT00750000117717.
    HOGENOMiHOG000234546.
    HOVERGENiHBG006180.
    InParanoidiQ3UZB9.
    OMAiKVTWGSR.
    OrthoDBiEOG7H1JM1.
    TreeFamiTF319371.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR008422. Homeobox_KN_domain.
    IPR009057. Homeodomain-like.
    IPR003893. Iroquois_homeo.
    [Graphical view]
    PfamiPF05920. Homeobox_KN. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    SM00548. IRO. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P81068-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFPQLGYPQ YLSAAGPGAY GGERPGVLAA AAAAAAAASS GRPGTAELGA    50
    GAGAAAVTSV LGMYAAAGPY AGAPNYSAFL PYAADLSLFS QMGSQYELKD 100
    NPGVHPATFA AHTTPAYYPY GQFQYGDPGR PKNATRESTS TLKAWLNEHR 150
    KNPYPTKGEK IMLAIITKMT LTQVSTWFAN ARRRLKKENK VTWGARSKDQ 200
    EDGALFGSDT EGDPEKAEDD EEIDLESIDI DQIDERDGDQ SNEDEEDKAE 250
    APRARVAPPA SARDQSSPLS AAETLKSQDS PLGLVKEVSE PGSTRLLSPG 300
    AAAVGLQGAP HSKPKIWSLA ETATSPDGAP KASPPPPSSH ASAHGPPSGS 350
    PLQHPAFLPS HGLYTCHIGK FSNWTNGAFL AQGSLLNMRS FLGVSAPHAA 400
    PHGPHLTAPP PPQPPVQVAT GVLHGEKASA RSSPALPERD LVTRPDSPPQ 450
    QLKSPFQPVR DNSLAPQEGT PRILAALPSA 480
    Length:480
    Mass (Da):50,169
    Last modified:October 3, 2012 - v4
    Checksum:i38D141E4C127A6C1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731A → G in BAE21938. (PubMed:16141072)Curated
    Sequence conflicti197 – 1971S → T in AAF63954. (PubMed:10926765)Curated
    Sequence conflicti257 – 2571A → R in AAF63954. (PubMed:10926765)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF165984 mRNA. Translation: AAF63954.1.
    AK133937 mRNA. Translation: BAE21938.1.
    CT009684 Genomic DNA. No translation available.
    CH466563 Genomic DNA. Translation: EDL37041.1.
    Y15002 mRNA. Translation: CAA75234.1.
    CCDSiCCDS26627.1.
    RefSeqiNP_034703.2. NM_010573.2.
    UniGeneiMm.316056.

    Genome annotation databases

    EnsembliENSMUST00000077337; ENSMUSP00000076562; ENSMUSG00000060969.
    GeneIDi16371.
    KEGGimmu:16371.
    UCSCiuc007rdd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF165984 mRNA. Translation: AAF63954.1 .
    AK133937 mRNA. Translation: BAE21938.1 .
    CT009684 Genomic DNA. No translation available.
    CH466563 Genomic DNA. Translation: EDL37041.1 .
    Y15002 mRNA. Translation: CAA75234.1 .
    CCDSi CCDS26627.1.
    RefSeqi NP_034703.2. NM_010573.2.
    UniGenei Mm.316056.

    3D structure databases

    ProteinModelPortali P81068.
    SMRi P81068. Positions 136-185.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P81068.

    Proteomic databases

    PRIDEi P81068.

    Protocols and materials databases

    DNASUi 16371.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000077337 ; ENSMUSP00000076562 ; ENSMUSG00000060969 .
    GeneIDi 16371.
    KEGGi mmu:16371.
    UCSCi uc007rdd.1. mouse.

    Organism-specific databases

    CTDi 79192.
    MGIi MGI:1197515. Irx1.

    Phylogenomic databases

    eggNOGi NOG279452.
    GeneTreei ENSGT00750000117717.
    HOGENOMi HOG000234546.
    HOVERGENi HBG006180.
    InParanoidi Q3UZB9.
    OMAi KVTWGSR.
    OrthoDBi EOG7H1JM1.
    TreeFami TF319371.

    Miscellaneous databases

    ChiTaRSi IRX1. mouse.
    NextBioi 289496.
    PROi P81068.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_IRX1.
    Genevestigatori P81068.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR008422. Homeobox_KN_domain.
    IPR009057. Homeodomain-like.
    IPR003893. Iroquois_homeo.
    [Graphical view ]
    Pfami PF05920. Homeobox_KN. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    SM00548. IRO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart."
      Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W., Moorman A.F.M.
      Dev. Biol. 224:263-274(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Strain: FVB/N.
      Tissue: Embryonic heart.
    2. "Expression of two novel mouse Iroquois-class homeobox genes during neurogenesis."
      Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.
      Mech. Dev. 91:317-321(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "Identification of the vertebrate Iroquois homeobox gene family with overlapping expression during early development of the nervous system."
      Bosse A., Zulch A., Becker M.B., Torres M., Gomez-Skarmeta J.-L., Modolell J., Gruss P.
      Mech. Dev. 69:169-181(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-198, DEVELOPMENTAL STAGE.
    7. "The prepattern transcription factor Irx3 directs nephron segment identity."
      Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.
      Genes Dev. 21:2358-2370(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiIRX1_MOUSE
    AccessioniPrimary (citable) accession number: P81068
    Secondary accession number(s): G5E8H6
    , O55120, Q3UZB9, Q9JLL6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 105 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3