Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Iroquois-class homeodomain protein IRX-1

Gene

Irx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi127 – 18963Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. metanephros development Source: UniProtKB
  2. proximal/distal pattern formation involved in metanephric nephron development Source: UniProtKB
  3. regulation of transcription, DNA-templated Source: InterPro
  4. specification of loop of Henle identity Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Iroquois-class homeodomain protein IRX-1
Alternative name(s):
Homeodomain protein IRXA1
Iroquois homeobox protein 1
Gene namesi
Name:Irx1
Synonyms:Irxa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:1197515. Irx1.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Iroquois-class homeodomain protein IRX-1PRO_0000049152Add
BLAST

Proteomic databases

PRIDEiP81068.

PTM databases

PhosphoSiteiP81068.

Expressioni

Tissue specificityi

Expressed in specific and overlapping patterns with Irx1 and Irx2 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule.1 Publication

Developmental stagei

First detected in the mesoderm at stage E7.5. During neurogenesis (E9.5 to E10.5), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon, rhombencephalon and spinal cord, with expression excluded from the midbrain-hindbrain junction. Beginning at E9.5, expression is found in the epithelial component of the branchial arches and foregut. At later stages, expressed in somites. At E10.5, expressed in the cephalic mesenchyme surrounding the optic vesicle. By E12.5, expression remains in the mesenchyme and also begins in the neuroretina, and at E16.5 expression is exclusively located in the inner neuroblast layers of the neuroretina. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from E10.5 onwards. Expressed in distinct patterns in the developing limb buds from E10.5 onwards. Expressed in the developing heart in the ventricular septum from the onset of its formation (E10.5) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system.3 Publications

Gene expression databases

CleanExiMM_IRX1.
GenevestigatoriP81068.

Structurei

3D structure databases

ProteinModelPortaliP81068.
SMRiP81068. Positions 136-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 3810Poly-Ala
Compositional biasi334 – 3374Poly-Pro
Compositional biasi409 – 4157Poly-Pro

Sequence similaritiesi

Belongs to the TALE/IRO homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG279452.
GeneTreeiENSGT00750000117365.
HOGENOMiHOG000234546.
HOVERGENiHBG006180.
InParanoidiP81068.
OMAiKVTWGSR.
OrthoDBiEOG7H1JM1.
TreeFamiTF319371.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P81068-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFPQLGYPQ YLSAAGPGAY GGERPGVLAA AAAAAAAASS GRPGTAELGA
60 70 80 90 100
GAGAAAVTSV LGMYAAAGPY AGAPNYSAFL PYAADLSLFS QMGSQYELKD
110 120 130 140 150
NPGVHPATFA AHTTPAYYPY GQFQYGDPGR PKNATRESTS TLKAWLNEHR
160 170 180 190 200
KNPYPTKGEK IMLAIITKMT LTQVSTWFAN ARRRLKKENK VTWGARSKDQ
210 220 230 240 250
EDGALFGSDT EGDPEKAEDD EEIDLESIDI DQIDERDGDQ SNEDEEDKAE
260 270 280 290 300
APRARVAPPA SARDQSSPLS AAETLKSQDS PLGLVKEVSE PGSTRLLSPG
310 320 330 340 350
AAAVGLQGAP HSKPKIWSLA ETATSPDGAP KASPPPPSSH ASAHGPPSGS
360 370 380 390 400
PLQHPAFLPS HGLYTCHIGK FSNWTNGAFL AQGSLLNMRS FLGVSAPHAA
410 420 430 440 450
PHGPHLTAPP PPQPPVQVAT GVLHGEKASA RSSPALPERD LVTRPDSPPQ
460 470 480
QLKSPFQPVR DNSLAPQEGT PRILAALPSA
Length:480
Mass (Da):50,169
Last modified:October 3, 2012 - v4
Checksum:i38D141E4C127A6C1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731A → G in BAE21938. (PubMed:16141072)Curated
Sequence conflicti197 – 1971S → T in AAF63954. (PubMed:10926765)Curated
Sequence conflicti257 – 2571A → R in AAF63954. (PubMed:10926765)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165984 mRNA. Translation: AAF63954.1.
AK133937 mRNA. Translation: BAE21938.1.
CT009684 Genomic DNA. No translation available.
CH466563 Genomic DNA. Translation: EDL37041.1.
Y15002 mRNA. Translation: CAA75234.1.
CCDSiCCDS26627.1.
RefSeqiNP_034703.2. NM_010573.2.
UniGeneiMm.316056.

Genome annotation databases

EnsembliENSMUST00000077337; ENSMUSP00000076562; ENSMUSG00000060969.
GeneIDi16371.
KEGGimmu:16371.
UCSCiuc007rdd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165984 mRNA. Translation: AAF63954.1.
AK133937 mRNA. Translation: BAE21938.1.
CT009684 Genomic DNA. No translation available.
CH466563 Genomic DNA. Translation: EDL37041.1.
Y15002 mRNA. Translation: CAA75234.1.
CCDSiCCDS26627.1.
RefSeqiNP_034703.2. NM_010573.2.
UniGeneiMm.316056.

3D structure databases

ProteinModelPortaliP81068.
SMRiP81068. Positions 136-185.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP81068.

Proteomic databases

PRIDEiP81068.

Protocols and materials databases

DNASUi16371.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077337; ENSMUSP00000076562; ENSMUSG00000060969.
GeneIDi16371.
KEGGimmu:16371.
UCSCiuc007rdd.1. mouse.

Organism-specific databases

CTDi79192.
MGIiMGI:1197515. Irx1.

Phylogenomic databases

eggNOGiNOG279452.
GeneTreeiENSGT00750000117365.
HOGENOMiHOG000234546.
HOVERGENiHBG006180.
InParanoidiP81068.
OMAiKVTWGSR.
OrthoDBiEOG7H1JM1.
TreeFamiTF319371.

Miscellaneous databases

ChiTaRSiIrx1. mouse.
NextBioi289496.
PROiP81068.
SOURCEiSearch...

Gene expression databases

CleanExiMM_IRX1.
GenevestigatoriP81068.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart."
    Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W., Moorman A.F.M.
    Dev. Biol. 224:263-274(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: FVB/N.
    Tissue: Embryonic heart.
  2. "Expression of two novel mouse Iroquois-class homeobox genes during neurogenesis."
    Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.
    Mech. Dev. 91:317-321(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "Identification of the vertebrate Iroquois homeobox gene family with overlapping expression during early development of the nervous system."
    Bosse A., Zulch A., Becker M.B., Torres M., Gomez-Skarmeta J.-L., Modolell J., Gruss P.
    Mech. Dev. 69:169-181(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-198, DEVELOPMENTAL STAGE.
  7. "The prepattern transcription factor Irx3 directs nephron segment identity."
    Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.
    Genes Dev. 21:2358-2370(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiIRX1_MOUSE
AccessioniPrimary (citable) accession number: P81068
Secondary accession number(s): G5E8H6
, O55120, Q3UZB9, Q9JLL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 3, 2012
Last modified: January 7, 2015
This is version 108 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.