Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P81068 (IRX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Iroquois-class homeodomain protein IRX-1
Alternative name(s):
Homeodomain protein IRXA1
Iroquois homeobox protein 1
Gene names
Name:Irx1
Synonyms:Irxa1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in specific and overlapping patterns with Irx1 and Irx2 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule. Ref.7

Developmental stage

First detected in the mesoderm at stage E7.5. During neurogenesis (E9.5 to E10.5), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon, rhombencephalon and spinal cord, with expression excluded from the midbrain-hindbrain junction. Beginning at E9.5, expression is found in the epithelial component of the branchial arches and foregut. At later stages, expressed in somites. At E10.5, expressed in the cephalic mesenchyme surrounding the optic vesicle. By E12.5, expression remains in the mesenchyme and also begins in the neuroretina, and at E16.5 expression is exclusively located in the inner neuroblast layers of the neuroretina. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from E10.5 onwards. Expressed in distinct patterns in the developing limb buds from E10.5 onwards. Expressed in the developing heart in the ventricular septum from the onset of its formation (E10.5) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Ref.1 Ref.2 Ref.6

Sequence similarities

Belongs to the TALE/IRO homeobox family.

Contains 1 homeobox DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 480480Iroquois-class homeodomain protein IRX-1
PRO_0000049152

Regions

DNA binding127 – 18963Homeobox; TALE-type
Compositional bias29 – 3810Poly-Ala
Compositional bias334 – 3374Poly-Pro
Compositional bias409 – 4157Poly-Pro

Experimental info

Sequence conflict731A → G in BAE21938. Ref.3
Sequence conflict1971S → T in AAF63954. Ref.1
Sequence conflict2571A → R in AAF63954. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P81068 [UniParc].

Last modified October 3, 2012. Version 4.
Checksum: 38D141E4C127A6C1

FASTA48050,169
        10         20         30         40         50         60 
MSFPQLGYPQ YLSAAGPGAY GGERPGVLAA AAAAAAAASS GRPGTAELGA GAGAAAVTSV 

        70         80         90        100        110        120 
LGMYAAAGPY AGAPNYSAFL PYAADLSLFS QMGSQYELKD NPGVHPATFA AHTTPAYYPY 

       130        140        150        160        170        180 
GQFQYGDPGR PKNATRESTS TLKAWLNEHR KNPYPTKGEK IMLAIITKMT LTQVSTWFAN 

       190        200        210        220        230        240 
ARRRLKKENK VTWGARSKDQ EDGALFGSDT EGDPEKAEDD EEIDLESIDI DQIDERDGDQ 

       250        260        270        280        290        300 
SNEDEEDKAE APRARVAPPA SARDQSSPLS AAETLKSQDS PLGLVKEVSE PGSTRLLSPG 

       310        320        330        340        350        360 
AAAVGLQGAP HSKPKIWSLA ETATSPDGAP KASPPPPSSH ASAHGPPSGS PLQHPAFLPS 

       370        380        390        400        410        420 
HGLYTCHIGK FSNWTNGAFL AQGSLLNMRS FLGVSAPHAA PHGPHLTAPP PPQPPVQVAT 

       430        440        450        460        470        480 
GVLHGEKASA RSSPALPERD LVTRPDSPPQ QLKSPFQPVR DNSLAPQEGT PRILAALPSA 

« Hide

References

« Hide 'large scale' references
[1]"Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart."
Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W., Moorman A.F.M.
Dev. Biol. 224:263-274(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: FVB/N.
Tissue: Embryonic heart.
[2]"Expression of two novel mouse Iroquois-class homeobox genes during neurogenesis."
Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.
Mech. Dev. 91:317-321(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Identification of the vertebrate Iroquois homeobox gene family with overlapping expression during early development of the nervous system."
Bosse A., Zulch A., Becker M.B., Torres M., Gomez-Skarmeta J.-L., Modolell J., Gruss P.
Mech. Dev. 69:169-181(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-198, DEVELOPMENTAL STAGE.
[7]"The prepattern transcription factor Irx3 directs nephron segment identity."
Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.
Genes Dev. 21:2358-2370(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF165984 mRNA. Translation: AAF63954.1.
AK133937 mRNA. Translation: BAE21938.1.
CT009684 Genomic DNA. No translation available.
CH466563 Genomic DNA. Translation: EDL37041.1.
Y15002 mRNA. Translation: CAA75234.1.
CCDSCCDS26627.1.
RefSeqNP_034703.2. NM_010573.2.
UniGeneMm.316056.

3D structure databases

ProteinModelPortalP81068.
SMRP81068. Positions 136-185.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP81068.

Proteomic databases

PRIDEP81068.

Protocols and materials databases

DNASU16371.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000077337; ENSMUSP00000076562; ENSMUSG00000060969.
GeneID16371.
KEGGmmu:16371.
UCSCuc007rdd.1. mouse.

Organism-specific databases

CTD79192.
MGIMGI:1197515. Irx1.

Phylogenomic databases

eggNOGNOG279452.
GeneTreeENSGT00750000117717.
HOGENOMHOG000234546.
HOVERGENHBG006180.
InParanoidQ3UZB9.
OMAKVTWGSR.
OrthoDBEOG7H1JM1.
TreeFamTF319371.

Gene expression databases

CleanExMM_IRX1.
GenevestigatorP81068.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIRX1. mouse.
NextBio289496.
PROP81068.
SOURCESearch...

Entry information

Entry nameIRX1_MOUSE
AccessionPrimary (citable) accession number: P81068
Secondary accession number(s): G5E8H6 expand/collapse secondary AC list , O55120, Q3UZB9, Q9JLL6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 104 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot