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Protein

Iroquois-class homeodomain protein IRX-2

Gene

Irx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Caution

Called Irx6 by PubMed:10704856.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi115 – 177Homeobox; TALE-typePROSITE-ProRule annotationAdd BLAST63

GO - Molecular functioni

GO - Biological processi

  • metanephros development Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • proximal/distal pattern formation involved in metanephric nephron development Source: UniProtKB
  • specification of loop of Henle identity Source: UniProtKB

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Iroquois-class homeodomain protein IRX-2
Alternative name(s):
Homeodomain protein IRXA2
Iroquois homeobox protein 2
Iroquois-class homeobox protein Irx6
Gene namesi
Name:Irx2
Synonyms:Irx6, Irxa2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1197526 Irx2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000491541 – 474Iroquois-class homeodomain protein IRX-2Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP81066
PRIDEiP81066

PTM databases

iPTMnetiP81066
PhosphoSitePlusiP81066

Expressioni

Tissue specificityi

Expressed in specific and overlapping patterns with Irx1 and Irx3 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule.1 Publication

Developmental stagei

First expressed at E8.0. During neural tube closure (E8.5), expression appears for the first time in the rhombencephalon in the presumptive region of future rhombomere 4. During neurogenesis (E9.5 to E10.5), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon and rhombencephalon. Expression is strong in the tectum of the mesencephalon and in the hindbrain, expression is restricted to rhombomeres. Expression in the spinal cord is weak and confined to the alar plate. Beginning at E9.5, expressed in the epithelial component of the branchial arches and foregut. At E10.5, expression extends rostrally into the dorsal diencephalon. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from E10.5 onwards. From E10.5 onwards, weak expression begins in the limb bud. Also expressed in other tissues during organogenesis; at E9.5, expressed in the superficial ectoderm surrounding the body and in the region of the foregut, which will form the pharynx and the lung bud. at E10.5, found in the cephalic mesenchyme around the optic vesicle. By E12.5, still expressed in the mesenchyme, and expression begins in specific subsets of post-mitotic cells in the neuroretina. As development ensues, expression increases in the neuroretina and mesenchymal expression gradually decreases. At E16.5, expressed exclusively in the inner neuroblast layers of the neuroretina. Expressed in the developing heart in the ventricular septum from the onset of its formation (E10.5) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system.3 Publications

Gene expression databases

BgeeiENSMUSG00000001504
CleanExiMM_IRX2
ExpressionAtlasiP81066 baseline and differential
GenevisibleiP81066 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073976

Structurei

3D structure databases

ProteinModelPortaliP81066
SMRiP81066
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 78Ala-richAdd BLAST57

Sequence similaritiesi

Belongs to the TALE/IRO homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0773 Eukaryota
ENOG410XPMQ LUCA
GeneTreeiENSGT00750000117365
HOGENOMiHOG000234546
HOVERGENiHBG006180
InParanoidiP81066
OMAiSYMGAPY
OrthoDBiEOG091G15AO
PhylomeDBiP81066
TreeFamiTF319371

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR008422 Homeobox_KN_domain
IPR003893 Iroquois_homeo
PfamiView protein in Pfam
PF05920 Homeobox_KN, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00548 IRO, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P81066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYPQGYLYQ APGSLALYSC PAYGASALAA PRSEELARSA SGSAFSPYPG
60 70 80 90 100
SAAFTAQAAT GFGSPLQYSA DAAAAAAAGF PSYVGSPYDT HTTGMTGAIS
110 120 130 140 150
YHPYGSAAYP YQLNDPAYRK NATRDATATL KAWLNEHRKN PYPTKGEKIM
160 170 180 190 200
LAIITKMTLT QVSTWFANAR RRLKKENKMT WAPRNKSEDE DEDEGDASRS
210 220 230 240 250
KEESSDKAQD GTETSAEDEG ISLHVDSLTD HSCSAESDGE KLPCRAGDAL
260 270 280 290 300
CESGSECKDK FEDLEDEEDE EDECERDLAP PKPVTSSPLT GVEAPLLSPA
310 320 330 340 350
PEAAPRGGSG GKTPLGSRTS PGAPPPASKP KLWSLAEIAT SDLKQPSLGP
360 370 380 390 400
GCGPPGLPAA AAPASTGAPP GGSPYSASPL LGRHLYYTSP FYGNYTNYGN
410 420 430 440 450
LNAALQGQGL LRYNTAASSP GETLHAMPKA ASDTGKAGSH SLESHYRPPG
460 470
GGYEPKKDTS EGCAVVGAGV QTYL
Length:474
Mass (Da):49,484
Last modified:April 16, 2002 - v2
Checksum:i04E39C42E717F98B
GO

Sequence cautioni

The sequence AAF63956 differs from that shown. Reason: Frameshift at positions 7, 44, 50, 73, 77 and 472.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27 – 33ALAAPRS → GGARA in AAF63956 (PubMed:10704856).Curated7
Sequence conflicti185 – 186NK → EQ (PubMed:9486539).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295369 mRNA Translation: AAG10083.1
AF165986 mRNA Translation: AAF63956.1 Frameshift.
AK148520 mRNA Translation: BAE28598.1
BC029750 mRNA Translation: AAH29750.1
Y15000 mRNA Translation: CAA75232.1 Sequence problems.
CCDSiCCDS26628.1
RefSeqiNP_034704.1, NM_010574.3
UniGeneiMm.28888

Genome annotation databases

EnsembliENSMUST00000074372; ENSMUSP00000073976; ENSMUSG00000001504
GeneIDi16372
KEGGimmu:16372
UCSCiuc007rde.1 mouse

Similar proteinsi

Entry informationi

Entry nameiIRX2_MOUSE
AccessioniPrimary (citable) accession number: P81066
Secondary accession number(s): O55121
, Q3UFG3, Q9ERN1, Q9JLL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 16, 2002
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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