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P81066

- IRX2_MOUSE

UniProt

P81066 - IRX2_MOUSE

Protein

Iroquois-class homeodomain protein IRX-2

Gene

Irx2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (16 Apr 2002)
      Previous versions | rss
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    Functioni

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi115 – 17763Homeobox; TALE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. metanephros development Source: UniProtKB
    2. proximal/distal pattern formation involved in metanephric nephron development Source: UniProtKB
    3. specification of loop of Henle identity Source: UniProtKB

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iroquois-class homeodomain protein IRX-2
    Alternative name(s):
    Homeodomain protein IRXA2
    Iroquois homeobox protein 2
    Iroquois-class homeobox protein Irx6
    Gene namesi
    Name:Irx2
    Synonyms:Irx6, Irxa2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1197526. Irx2.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Iroquois-class homeodomain protein IRX-2PRO_0000049154Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei187 – 1871PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP81066.

    PTM databases

    PhosphoSiteiP81066.

    Expressioni

    Tissue specificityi

    Expressed in specific and overlapping patterns with Irx1 and Irx3 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule.1 Publication

    Developmental stagei

    First expressed at E8.0. During neural tube closure (E8.5), expression appears for the first time in the rhombencephalon in the presumptive region of future rhombomere 4. During neurogenesis (E9.5 to E10.5), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon and rhombencephalon. Expression is strong in the tectum of the mesencephalon and in the hindbrain, expression is restricted to rhombomeres. Expression in the spinal cord is weak and confined to the alar plate. Beginning at E9.5, expressed in the epithelial component of the branchial arches and foregut. At E10.5, expression extends rostrally into the dorsal diencephalon. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from E10.5 onwards. From E10.5 onwards, weak expression begins in the limb bud. Also expressed in other tissues during organogenesis; at E9.5, expressed in the superficial ectoderm surrounding the body and in the region of the foregut, which will form the pharynx and the lung bud. at E10.5, found in the cephalic mesenchyme around the optic vesicle. By E12.5, still expressed in the mesenchyme, and expression begins in specific subsets of post-mitotic cells in the neuroretina. As development ensues, expression increases in the neuroretina and mesenchymal expression gradually decreases. At E16.5, expressed exclusively in the inner neuroblast layers of the neuroretina. Expressed in the developing heart in the ventricular septum from the onset of its formation (E10.5) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system.3 Publications

    Gene expression databases

    ArrayExpressiP81066.
    BgeeiP81066.
    CleanExiMM_IRX2.
    GenevestigatoriP81066.

    Structurei

    3D structure databases

    ProteinModelPortaliP81066.
    SMRiP81066. Positions 126-173.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 7857Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TALE/IRO homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG310996.
    GeneTreeiENSGT00750000117365.
    HOGENOMiHOG000234546.
    HOVERGENiHBG006180.
    InParanoidiQ9JLL4.
    OMAiEDECERD.
    OrthoDBiEOG7TJ3HS.
    PhylomeDBiP81066.
    TreeFamiTF319371.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR008422. Homeobox_KN_domain.
    IPR009057. Homeodomain-like.
    IPR003893. Iroquois_homeo.
    [Graphical view]
    PfamiPF05920. Homeobox_KN. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    SM00548. IRO. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P81066-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYPQGYLYQ APGSLALYSC PAYGASALAA PRSEELARSA SGSAFSPYPG    50
    SAAFTAQAAT GFGSPLQYSA DAAAAAAAGF PSYVGSPYDT HTTGMTGAIS 100
    YHPYGSAAYP YQLNDPAYRK NATRDATATL KAWLNEHRKN PYPTKGEKIM 150
    LAIITKMTLT QVSTWFANAR RRLKKENKMT WAPRNKSEDE DEDEGDASRS 200
    KEESSDKAQD GTETSAEDEG ISLHVDSLTD HSCSAESDGE KLPCRAGDAL 250
    CESGSECKDK FEDLEDEEDE EDECERDLAP PKPVTSSPLT GVEAPLLSPA 300
    PEAAPRGGSG GKTPLGSRTS PGAPPPASKP KLWSLAEIAT SDLKQPSLGP 350
    GCGPPGLPAA AAPASTGAPP GGSPYSASPL LGRHLYYTSP FYGNYTNYGN 400
    LNAALQGQGL LRYNTAASSP GETLHAMPKA ASDTGKAGSH SLESHYRPPG 450
    GGYEPKKDTS EGCAVVGAGV QTYL 474
    Length:474
    Mass (Da):49,484
    Last modified:April 16, 2002 - v2
    Checksum:i04E39C42E717F98B
    GO

    Sequence cautioni

    The sequence AAF63956.1 differs from that shown. Reason: Frameshift at positions 7, 44, 50, 73, 77 and 472.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 337ALAAPRS → GGARA in AAF63956. (PubMed:10704856)Curated
    Sequence conflicti185 – 1862NK → EQ(PubMed:9486539)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF295369 mRNA. Translation: AAG10083.1.
    AF165986 mRNA. Translation: AAF63956.1. Frameshift.
    AK148520 mRNA. Translation: BAE28598.1.
    BC029750 mRNA. Translation: AAH29750.1.
    Y15000 mRNA. Translation: CAA75232.1. Sequence problems.
    CCDSiCCDS26628.1.
    RefSeqiNP_034704.1. NM_010574.2.
    UniGeneiMm.28888.

    Genome annotation databases

    EnsembliENSMUST00000074372; ENSMUSP00000073976; ENSMUSG00000001504.
    GeneIDi16372.
    KEGGimmu:16372.
    UCSCiuc007rde.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF295369 mRNA. Translation: AAG10083.1 .
    AF165986 mRNA. Translation: AAF63956.1 . Frameshift.
    AK148520 mRNA. Translation: BAE28598.1 .
    BC029750 mRNA. Translation: AAH29750.1 .
    Y15000 mRNA. Translation: CAA75232.1 . Sequence problems.
    CCDSi CCDS26628.1.
    RefSeqi NP_034704.1. NM_010574.2.
    UniGenei Mm.28888.

    3D structure databases

    ProteinModelPortali P81066.
    SMRi P81066. Positions 126-173.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P81066.

    Proteomic databases

    PRIDEi P81066.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000074372 ; ENSMUSP00000073976 ; ENSMUSG00000001504 .
    GeneIDi 16372.
    KEGGi mmu:16372.
    UCSCi uc007rde.1. mouse.

    Organism-specific databases

    CTDi 153572.
    MGIi MGI:1197526. Irx2.

    Phylogenomic databases

    eggNOGi NOG310996.
    GeneTreei ENSGT00750000117365.
    HOGENOMi HOG000234546.
    HOVERGENi HBG006180.
    InParanoidi Q9JLL4.
    OMAi EDECERD.
    OrthoDBi EOG7TJ3HS.
    PhylomeDBi P81066.
    TreeFami TF319371.

    Miscellaneous databases

    ChiTaRSi IRX2. mouse.
    NextBioi 289500.
    PROi P81066.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P81066.
    Bgeei P81066.
    CleanExi MM_IRX2.
    Genevestigatori P81066.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR008422. Homeobox_KN_domain.
    IPR009057. Homeodomain-like.
    IPR003893. Iroquois_homeo.
    [Graphical view ]
    Pfami PF05920. Homeobox_KN. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    SM00548. IRO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart."
      Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W., Moorman A.F.M.
      Dev. Biol. 224:263-274(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Strain: FVB/N.
      Tissue: Embryonic heart.
    2. "Expression of two novel mouse Iroquois-class homeobox genes during neurogenesis."
      Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.
      Mech. Dev. 91:317-321(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Pancreas.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Identification of the vertebrate Iroquois homeobox gene family with overlapping expression during early development of the nervous system."
      Bosse A., Zulch A., Becker M.B., Torres M., Gomez-Skarmeta J.-L., Modolell J., Gruss P.
      Mech. Dev. 69:169-181(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-186, DEVELOPMENTAL STAGE.
    6. "The prepattern transcription factor Irx3 directs nephron segment identity."
      Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.
      Genes Dev. 21:2358-2370(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiIRX2_MOUSE
    AccessioniPrimary (citable) accession number: P81066
    Secondary accession number(s): O55121
    , Q3UFG3, Q9ERN1, Q9JLL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: April 16, 2002
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Called Irx6 by PubMed:10704856.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3