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P81066 (IRX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Iroquois-class homeodomain protein IRX-2
Alternative name(s):
Homeodomain protein IRXA2
Iroquois homeobox protein 2
Iroquois-class homeobox protein Irx6
Gene names
Name:Irx2
Synonyms:Irx6, Irxa2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in specific and overlapping patterns with Irx1 and Irx3 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule. Ref.6

Developmental stage

First expressed at E8.0. During neural tube closure (E8.5), expression appears for the first time in the rhombencephalon in the presumptive region of future rhombomere 4. During neurogenesis (E9.5 to E10.5), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon and rhombencephalon. Expression is strong in the tectum of the mesencephalon and in the hindbrain, expression is restricted to rhombomeres. Expression in the spinal cord is weak and confined to the alar plate. Beginning at E9.5, expressed in the epithelial component of the branchial arches and foregut. At E10.5, expression extends rostrally into the dorsal diencephalon. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from E10.5 onwards. From E10.5 onwards, weak expression begins in the limb bud. Also expressed in other tissues during organogenesis; at E9.5, expressed in the superficial ectoderm surrounding the body and in the region of the foregut, which will form the pharynx and the lung bud. at E10.5, found in the cephalic mesenchyme around the optic vesicle. By E12.5, still expressed in the mesenchyme, and expression begins in specific subsets of post-mitotic cells in the neuroretina. As development ensues, expression increases in the neuroretina and mesenchymal expression gradually decreases. At E16.5, expressed exclusively in the inner neuroblast layers of the neuroretina. Expressed in the developing heart in the ventricular septum from the onset of its formation (E10.5) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Ref.1 Ref.2 Ref.5

Sequence similarities

Belongs to the TALE/IRO homeobox family.

Contains 1 homeobox DNA-binding domain.

Caution

Called Irx6 by Ref.2.

Sequence caution

The sequence AAF63956.1 differs from that shown. Reason: Frameshift at positions 7, 44, 50, 73, 77 and 472.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Iroquois-class homeodomain protein IRX-2
PRO_0000049154

Regions

DNA binding115 – 17763Homeobox; TALE-type
Compositional bias22 – 7857Ala-rich

Amino acid modifications

Modified residue1871Phosphoserine By similarity

Experimental info

Sequence conflict27 – 337ALAAPRS → GGARA in AAF63956. Ref.2
Sequence conflict185 – 1862NK → EQ Ref.5

Sequences

Sequence LengthMass (Da)Tools
P81066 [UniParc].

Last modified April 16, 2002. Version 2.
Checksum: 04E39C42E717F98B

FASTA47449,484
        10         20         30         40         50         60 
MSYPQGYLYQ APGSLALYSC PAYGASALAA PRSEELARSA SGSAFSPYPG SAAFTAQAAT 

        70         80         90        100        110        120 
GFGSPLQYSA DAAAAAAAGF PSYVGSPYDT HTTGMTGAIS YHPYGSAAYP YQLNDPAYRK 

       130        140        150        160        170        180 
NATRDATATL KAWLNEHRKN PYPTKGEKIM LAIITKMTLT QVSTWFANAR RRLKKENKMT 

       190        200        210        220        230        240 
WAPRNKSEDE DEDEGDASRS KEESSDKAQD GTETSAEDEG ISLHVDSLTD HSCSAESDGE 

       250        260        270        280        290        300 
KLPCRAGDAL CESGSECKDK FEDLEDEEDE EDECERDLAP PKPVTSSPLT GVEAPLLSPA 

       310        320        330        340        350        360 
PEAAPRGGSG GKTPLGSRTS PGAPPPASKP KLWSLAEIAT SDLKQPSLGP GCGPPGLPAA 

       370        380        390        400        410        420 
AAPASTGAPP GGSPYSASPL LGRHLYYTSP FYGNYTNYGN LNAALQGQGL LRYNTAASSP 

       430        440        450        460        470 
GETLHAMPKA ASDTGKAGSH SLESHYRPPG GGYEPKKDTS EGCAVVGAGV QTYL 

« Hide

References

« Hide 'large scale' references
[1]"Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart."
Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W., Moorman A.F.M.
Dev. Biol. 224:263-274(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: FVB/N.
Tissue: Embryonic heart.
[2]"Expression of two novel mouse Iroquois-class homeobox genes during neurogenesis."
Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.
Mech. Dev. 91:317-321(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Pancreas.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[5]"Identification of the vertebrate Iroquois homeobox gene family with overlapping expression during early development of the nervous system."
Bosse A., Zulch A., Becker M.B., Torres M., Gomez-Skarmeta J.-L., Modolell J., Gruss P.
Mech. Dev. 69:169-181(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-186, DEVELOPMENTAL STAGE.
[6]"The prepattern transcription factor Irx3 directs nephron segment identity."
Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.
Genes Dev. 21:2358-2370(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF295369 mRNA. Translation: AAG10083.1.
AF165986 mRNA. Translation: AAF63956.1. Frameshift.
AK148520 mRNA. Translation: BAE28598.1.
BC029750 mRNA. Translation: AAH29750.1.
Y15000 mRNA. Translation: CAA75232.1. Sequence problems.
CCDSCCDS26628.1.
RefSeqNP_034704.1. NM_010574.2.
UniGeneMm.28888.

3D structure databases

ProteinModelPortalP81066.
SMRP81066. Positions 126-173.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP81066.

Proteomic databases

PRIDEP81066.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000074372; ENSMUSP00000073976; ENSMUSG00000001504.
GeneID16372.
KEGGmmu:16372.
UCSCuc007rde.1. mouse.

Organism-specific databases

CTD153572.
MGIMGI:1197526. Irx2.

Phylogenomic databases

eggNOGNOG310996.
GeneTreeENSGT00750000117365.
HOGENOMHOG000234546.
HOVERGENHBG006180.
InParanoidQ9JLL4.
OMAEDECERD.
OrthoDBEOG7TJ3HS.
PhylomeDBP81066.
TreeFamTF319371.

Gene expression databases

ArrayExpressP81066.
BgeeP81066.
CleanExMM_IRX2.
GenevestigatorP81066.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIRX2. mouse.
NextBio289500.
PROP81066.
SOURCESearch...

Entry information

Entry nameIRX2_MOUSE
AccessionPrimary (citable) accession number: P81066
Secondary accession number(s): O55121 expand/collapse secondary AC list , Q3UFG3, Q9ERN1, Q9JLL4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 16, 2002
Last modified: July 9, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot