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Protein

Iroquois-class homeodomain protein IRX-2

Gene

Irx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi115 – 17763Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • metanephros development Source: UniProtKB
  • proximal/distal pattern formation involved in metanephric nephron development Source: UniProtKB
  • regulation of transcription, DNA-templated Source: InterPro
  • specification of loop of Henle identity Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Iroquois-class homeodomain protein IRX-2
Alternative name(s):
Homeodomain protein IRXA2
Iroquois homeobox protein 2
Iroquois-class homeobox protein Irx6
Gene namesi
Name:Irx2
Synonyms:Irx6, Irxa2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1197526. Irx2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Iroquois-class homeodomain protein IRX-2PRO_0000049154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP81066.

PTM databases

PhosphoSiteiP81066.

Expressioni

Tissue specificityi

Expressed in specific and overlapping patterns with Irx1 and Irx3 in the developing and adult metanephric kidney. In the adult metanephros, renal expression is found in the loop of Henle in the S3 proximal tubule segment and in the thick ascending limb (TAL) of the distal tubule.1 Publication

Developmental stagei

First expressed at E8.0. During neural tube closure (E8.5), expression appears for the first time in the rhombencephalon in the presumptive region of future rhombomere 4. During neurogenesis (E9.5 to E10.5), predominantly expressed along the anteroposterior axis of the CNS in the mesencephalon, metencephalon and rhombencephalon. Expression is strong in the tectum of the mesencephalon and in the hindbrain, expression is restricted to rhombomeres. Expression in the spinal cord is weak and confined to the alar plate. Beginning at E9.5, expressed in the epithelial component of the branchial arches and foregut. At E10.5, expression extends rostrally into the dorsal diencephalon. Starting at the otic vesicle stage, shows regionalized expression in the developing inner ear with expression in the entire otic vesicle from E10.5 onwards. From E10.5 onwards, weak expression begins in the limb bud. Also expressed in other tissues during organogenesis; at E9.5, expressed in the superficial ectoderm surrounding the body and in the region of the foregut, which will form the pharynx and the lung bud. at E10.5, found in the cephalic mesenchyme around the optic vesicle. By E12.5, still expressed in the mesenchyme, and expression begins in specific subsets of post-mitotic cells in the neuroretina. As development ensues, expression increases in the neuroretina and mesenchymal expression gradually decreases. At E16.5, expressed exclusively in the inner neuroblast layers of the neuroretina. Expressed in the developing heart in the ventricular septum from the onset of its formation (E10.5) onward. In fetal stages, expression becomes confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system.3 Publications

Gene expression databases

BgeeiP81066.
CleanExiMM_IRX2.
ExpressionAtlasiP81066. baseline and differential.
GenevisibleiP81066. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073976.

Structurei

3D structure databases

ProteinModelPortaliP81066.
SMRiP81066. Positions 126-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 7857Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the TALE/IRO homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG310996.
GeneTreeiENSGT00750000117365.
HOGENOMiHOG000234546.
HOVERGENiHBG006180.
InParanoidiP81066.
OMAiHPLESHY.
OrthoDBiEOG7TJ3HS.
PhylomeDBiP81066.
TreeFamiTF319371.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P81066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYPQGYLYQ APGSLALYSC PAYGASALAA PRSEELARSA SGSAFSPYPG
60 70 80 90 100
SAAFTAQAAT GFGSPLQYSA DAAAAAAAGF PSYVGSPYDT HTTGMTGAIS
110 120 130 140 150
YHPYGSAAYP YQLNDPAYRK NATRDATATL KAWLNEHRKN PYPTKGEKIM
160 170 180 190 200
LAIITKMTLT QVSTWFANAR RRLKKENKMT WAPRNKSEDE DEDEGDASRS
210 220 230 240 250
KEESSDKAQD GTETSAEDEG ISLHVDSLTD HSCSAESDGE KLPCRAGDAL
260 270 280 290 300
CESGSECKDK FEDLEDEEDE EDECERDLAP PKPVTSSPLT GVEAPLLSPA
310 320 330 340 350
PEAAPRGGSG GKTPLGSRTS PGAPPPASKP KLWSLAEIAT SDLKQPSLGP
360 370 380 390 400
GCGPPGLPAA AAPASTGAPP GGSPYSASPL LGRHLYYTSP FYGNYTNYGN
410 420 430 440 450
LNAALQGQGL LRYNTAASSP GETLHAMPKA ASDTGKAGSH SLESHYRPPG
460 470
GGYEPKKDTS EGCAVVGAGV QTYL
Length:474
Mass (Da):49,484
Last modified:April 16, 2002 - v2
Checksum:i04E39C42E717F98B
GO

Sequence cautioni

The sequence AAF63956.1 differs from that shown. Reason: Frameshift at positions 7, 44, 50, 73, 77 and 472. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 337ALAAPRS → GGARA in AAF63956 (PubMed:10704856).Curated
Sequence conflicti185 – 1862NK → EQ (PubMed:9486539).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295369 mRNA. Translation: AAG10083.1.
AF165986 mRNA. Translation: AAF63956.1. Frameshift.
AK148520 mRNA. Translation: BAE28598.1.
BC029750 mRNA. Translation: AAH29750.1.
Y15000 mRNA. Translation: CAA75232.1. Sequence problems.
CCDSiCCDS26628.1.
RefSeqiNP_034704.1. NM_010574.2.
UniGeneiMm.28888.

Genome annotation databases

EnsembliENSMUST00000074372; ENSMUSP00000073976; ENSMUSG00000001504.
GeneIDi16372.
KEGGimmu:16372.
UCSCiuc007rde.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295369 mRNA. Translation: AAG10083.1.
AF165986 mRNA. Translation: AAF63956.1. Frameshift.
AK148520 mRNA. Translation: BAE28598.1.
BC029750 mRNA. Translation: AAH29750.1.
Y15000 mRNA. Translation: CAA75232.1. Sequence problems.
CCDSiCCDS26628.1.
RefSeqiNP_034704.1. NM_010574.2.
UniGeneiMm.28888.

3D structure databases

ProteinModelPortaliP81066.
SMRiP81066. Positions 126-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073976.

PTM databases

PhosphoSiteiP81066.

Proteomic databases

PRIDEiP81066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074372; ENSMUSP00000073976; ENSMUSG00000001504.
GeneIDi16372.
KEGGimmu:16372.
UCSCiuc007rde.1. mouse.

Organism-specific databases

CTDi153572.
MGIiMGI:1197526. Irx2.

Phylogenomic databases

eggNOGiNOG310996.
GeneTreeiENSGT00750000117365.
HOGENOMiHOG000234546.
HOVERGENiHBG006180.
InParanoidiP81066.
OMAiHPLESHY.
OrthoDBiEOG7TJ3HS.
PhylomeDBiP81066.
TreeFamiTF319371.

Miscellaneous databases

ChiTaRSiIrx2. mouse.
NextBioi289500.
PROiP81066.
SOURCEiSearch...

Gene expression databases

BgeeiP81066.
CleanExiMM_IRX2.
ExpressionAtlasiP81066. baseline and differential.
GenevisibleiP81066. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart."
    Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W., Moorman A.F.M.
    Dev. Biol. 224:263-274(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: FVB/N.
    Tissue: Embryonic heart.
  2. "Expression of two novel mouse Iroquois-class homeobox genes during neurogenesis."
    Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.
    Mech. Dev. 91:317-321(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pancreas.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Identification of the vertebrate Iroquois homeobox gene family with overlapping expression during early development of the nervous system."
    Bosse A., Zulch A., Becker M.B., Torres M., Gomez-Skarmeta J.-L., Modolell J., Gruss P.
    Mech. Dev. 69:169-181(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-186, DEVELOPMENTAL STAGE.
  6. "The prepattern transcription factor Irx3 directs nephron segment identity."
    Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.
    Genes Dev. 21:2358-2370(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiIRX2_MOUSE
AccessioniPrimary (citable) accession number: P81066
Secondary accession number(s): O55121
, Q3UFG3, Q9ERN1, Q9JLL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 16, 2002
Last modified: July 22, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Called Irx6 by PubMed:10704856.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.