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Protein

Pituitary homeobox 3

Gene

Pitx3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi62 – 121HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • cellular response to glial cell derived neurotrophic factor Source: RGD
  • dopaminergic neuron differentiation Source: UniProtKB
  • lens development in camera-type eye Source: UniProtKB
  • lens fiber cell differentiation Source: Ensembl
  • lens morphogenesis in camera-type eye Source: UniProtKB
  • locomotory behavior Source: Ensembl
  • midbrain development Source: UniProtKB
  • negative regulation of gliogenesis Source: RGD
  • negative regulation of neurogenesis Source: RGD
  • neuron development Source: Ensembl
  • positive regulation of cell proliferation in midbrain Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • response to cocaine Source: RGD
  • response to immobilization stress Source: RGD
  • response to methamphetamine hydrochloride Source: RGD
  • response to morphine Source: RGD

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary homeobox 3
Alternative name(s):
Homeobox protein PITX3
Paired-like homeodomain transcription factor 3
Gene namesi
Name:Pitx3
Synonyms:Ptx3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3332 Pitx3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000492311 – 302Pituitary homeobox 3Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP81062

PTM databases

iPTMnetiP81062
PhosphoSitePlusiP81062

Expressioni

Tissue specificityi

Expression is restricted to the substantia nigra and ventral tegmental area in the midbrain. Expression increases in prenatally stressed adult offspring in the ventral tegmental area, whereas no changes are observed in the substantia nigra area (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000019194
GenevisibleiP81062 RN

Interactioni

Subunit structurei

Interacts with SFPQ.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026013

Structurei

3D structure databases

ProteinModelPortaliP81062
SMRiP81062
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi262 – 275OARPROSITE-ProRule annotationAdd BLAST14
Motifi268 – 272Nuclear localization signalSequence analysis5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi240 – 250Poly-AlaAdd BLAST11

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0486 Eukaryota
ENOG410ZZ6J LUCA
GeneTreeiENSGT00900000140776
HOGENOMiHOG000273886
HOVERGENiHBG068770
InParanoidiP81062
KOiK09357
OMAiLCKGGFA
OrthoDBiEOG091G0H7U
PhylomeDBiP81062
TreeFamiTF351940

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR016233 Homeobox_Pitx/unc30
IPR003654 OAR_dom
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF03826 OAR, 1 hit
PIRSFiPIRSF000563 Homeobox_protein_Pitx/Unc30, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS50803 OAR, 1 hit

Sequencei

Sequence statusi: Complete.

P81062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFGLLGEAE ARSPALSLSD AGTPHPPLPE HGCKGQEHSD SEKASASLPG
60 70 80 90 100
GSPEDGSLKK KQRRQRTHFT SQQLQELEAT FQRNRYPDMS TREEIAVWTN
110 120 130 140 150
LTEARVRVWF KNRRAKWRKR ERSQQAELCK GGFAAPLGGL VPPYEEVYPG
160 170 180 190 200
YTYGNWPPKA LAPPLAAKTF PFAFNSVNVG PLASQPVFSP PSSIAASMVP
210 220 230 240 250
SAAAAPGTVP GPGALQGLGG APPGLAPAAV SSGAVSCPYA SAAAAAAAAA
260 270 280 290 300
SSPYVYRDPC NSSLASLRLK AKQHASFSYP AVPGPPPAAN LSPCQYAVER

PV
Length:302
Mass (Da):31,729
Last modified:May 30, 2000 - v2
Checksum:iDE0E81863C44E714
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011005 mRNA Translation: CAA09455.2
RefSeqiNP_062120.1, NM_019247.1
XP_006231540.1, XM_006231478.2
UniGeneiRn.22092

Genome annotation databases

EnsembliENSRNOT00000026013; ENSRNOP00000026013; ENSRNOG00000019194
GeneIDi29609
KEGGirno:29609
UCSCiRGD:3332 rat

Similar proteinsi

Entry informationi

Entry nameiPITX3_RAT
AccessioniPrimary (citable) accession number: P81062
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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