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Protein

Pituitary homeobox 3

Gene

Pitx3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi62 – 121HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • cellular response to glial cell derived neurotrophic factor Source: RGD
  • dopaminergic neuron differentiation Source: UniProtKB
  • lens development in camera-type eye Source: UniProtKB
  • lens fiber cell differentiation Source: Ensembl
  • lens morphogenesis in camera-type eye Source: UniProtKB
  • locomotory behavior Source: Ensembl
  • midbrain development Source: UniProtKB
  • negative regulation of gliogenesis Source: RGD
  • negative regulation of neurogenesis Source: RGD
  • neuron development Source: Ensembl
  • positive regulation of cell proliferation in midbrain Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • response to cocaine Source: RGD
  • response to immobilization stress Source: RGD
  • response to methamphetamine hydrochloride Source: RGD
  • response to morphine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary homeobox 3
Alternative name(s):
Homeobox protein PITX3
Paired-like homeodomain transcription factor 3
Gene namesi
Name:Pitx3
Synonyms:Ptx3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3332. Pitx3.

Subcellular locationi

GO - Cellular componenti

  • neuronal cell body Source: RGD
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000492311 – 302Pituitary homeobox 3Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP81062.

PTM databases

iPTMnetiP81062.
PhosphoSitePlusiP81062.

Expressioni

Tissue specificityi

Expression is restricted to the substantia nigra and ventral tegmental area in the midbrain. Expression increases in prenatally stressed adult offspring in the ventral tegmental area, whereas no changes are observed in the substantia nigra area (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000019194.
GenevisibleiP81062. RN.

Interactioni

Subunit structurei

Interacts with SFPQ.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026013.

Structurei

3D structure databases

ProteinModelPortaliP81062.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi262 – 275OARPROSITE-ProRule annotationAdd BLAST14
Motifi268 – 272Nuclear localization signalSequence analysis5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi240 – 250Poly-AlaAdd BLAST11

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0486. Eukaryota.
ENOG410ZZ6J. LUCA.
GeneTreeiENSGT00820000126963.
HOGENOMiHOG000273886.
HOVERGENiHBG068770.
InParanoidiP81062.
KOiK09357.
OMAiLCKGGFA.
OrthoDBiEOG091G0H7U.
PhylomeDBiP81062.
TreeFamiTF351940.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P81062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFGLLGEAE ARSPALSLSD AGTPHPPLPE HGCKGQEHSD SEKASASLPG
60 70 80 90 100
GSPEDGSLKK KQRRQRTHFT SQQLQELEAT FQRNRYPDMS TREEIAVWTN
110 120 130 140 150
LTEARVRVWF KNRRAKWRKR ERSQQAELCK GGFAAPLGGL VPPYEEVYPG
160 170 180 190 200
YTYGNWPPKA LAPPLAAKTF PFAFNSVNVG PLASQPVFSP PSSIAASMVP
210 220 230 240 250
SAAAAPGTVP GPGALQGLGG APPGLAPAAV SSGAVSCPYA SAAAAAAAAA
260 270 280 290 300
SSPYVYRDPC NSSLASLRLK AKQHASFSYP AVPGPPPAAN LSPCQYAVER

PV
Length:302
Mass (Da):31,729
Last modified:May 30, 2000 - v2
Checksum:iDE0E81863C44E714
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011005 mRNA. Translation: CAA09455.2.
RefSeqiNP_062120.1. NM_019247.1.
XP_006231540.1. XM_006231478.2.
UniGeneiRn.22092.

Genome annotation databases

EnsembliENSRNOT00000026013; ENSRNOP00000026013; ENSRNOG00000019194.
GeneIDi29609.
KEGGirno:29609.
UCSCiRGD:3332. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011005 mRNA. Translation: CAA09455.2.
RefSeqiNP_062120.1. NM_019247.1.
XP_006231540.1. XM_006231478.2.
UniGeneiRn.22092.

3D structure databases

ProteinModelPortaliP81062.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026013.

PTM databases

iPTMnetiP81062.
PhosphoSitePlusiP81062.

Proteomic databases

PaxDbiP81062.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026013; ENSRNOP00000026013; ENSRNOG00000019194.
GeneIDi29609.
KEGGirno:29609.
UCSCiRGD:3332. rat.

Organism-specific databases

CTDi5309.
RGDi3332. Pitx3.

Phylogenomic databases

eggNOGiKOG0486. Eukaryota.
ENOG410ZZ6J. LUCA.
GeneTreeiENSGT00820000126963.
HOGENOMiHOG000273886.
HOVERGENiHBG068770.
InParanoidiP81062.
KOiK09357.
OMAiLCKGGFA.
OrthoDBiEOG091G0H7U.
PhylomeDBiP81062.
TreeFamiTF351940.

Miscellaneous databases

PROiP81062.

Gene expression databases

BgeeiENSRNOG00000019194.
GenevisibleiP81062. RN.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPITX3_RAT
AccessioniPrimary (citable) accession number: P81062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.