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Protein

Penaeidin-3a

Gene
N/A
Organism
Litopenaeus vannamei (Whiteleg shrimp) (Penaeus vannamei)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antibacterial activity against M.luteus and E.coli bacteria. Antifungal activity against N.crassa and F.oxysporum. Presents chitin-binding activity.2 Publications

GO - Molecular functioni

  • chitin binding Source: UniProtKB

GO - Biological processi

  • defense response to bacterium Source: UniProtKB
  • defense response to fungus Source: UniProtKB
  • killing of cells of other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Keywords - Ligandi

Chitin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Penaeidin-3a
Short name:
P3-a
Short name:
Pen-3a
OrganismiLitopenaeus vannamei (Whiteleg shrimp) (Penaeus vannamei)
Taxonomic identifieri6689 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaDendrobranchiataPenaeoideaPenaeidaeLitopenaeus

Subcellular locationi

  • Cytoplasmic granule 1 Publication

  • Note: Cytoplasmic granules of hemocytes and to a lesser extent in small granules of hemocytes.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19192 PublicationsAdd
BLAST
Chaini20 – 8162Penaeidin-3aPRO_0000023506Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Pyrrolidone carboxylic acid
Disulfide bondi51 ↔ 66
Disulfide bondi55 ↔ 73
Disulfide bondi67 ↔ 74
Modified residuei81 – 811Serine amide1 Publication

Keywords - PTMi

Amidation, Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Higher expression in hemocytes and to a lesser extent in heart, testis, gills, intestine, lymphonoid organ and hepatopancreas. Traces in eyes and subcuticular epithelium. Not present in the brain.1 Publication

Developmental stagei

Expression decreases 3 hours after microbial challenge to return to control levels after 12 hours and slightly increases after 24 hours.1 Publication

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 305Combined sources
Helixi60 – 7011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UEONMR-A20-82[»]
DisProtiDP00373.
ProteinModelPortaliP81058.
SMRiP81058. Positions 20-82.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81058.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 4719Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the penaeidin family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR009226. Penaeidin.
[Graphical view]
PfamiPF05927. Penaeidin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81058-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLVVCLVFL ASFALVCQGQ VYKGGYTRPI PRPPPFVRPL PGGPIGPYNG
60 70 80
CPVSCRGISF SQARSCCSRL GRCCHVGKGY SG
Length:82
Mass (Da):8,748
Last modified:July 15, 1998 - v1
Checksum:iE60E09BB305521FD
GO

Mass spectrometryi

Molecular mass is 6617.4 Da from positions 20 - 81. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14926 mRNA. Translation: CAA75143.1.
AF387661 mRNA. Translation: AAK73084.1.
AF387662 mRNA. Translation: AAK73085.1.
AF387663 mRNA. Translation: AAK73086.1.
AF390139 mRNA. Translation: AAK77532.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14926 mRNA. Translation: CAA75143.1.
AF387661 mRNA. Translation: AAK73084.1.
AF387662 mRNA. Translation: AAK73085.1.
AF387663 mRNA. Translation: AAK73086.1.
AF390139 mRNA. Translation: AAK77532.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UEONMR-A20-82[»]
DisProtiDP00373.
ProteinModelPortaliP81058.
SMRiP81058. Positions 20-82.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP81058.

Family and domain databases

InterProiIPR009226. Penaeidin.
[Graphical view]
PfamiPF05927. Penaeidin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus vannamei (Decapoda)."
    Destoumieux D., Bulet P., Loew D., van Dorsselaer A., Rodriguez J., Bachere E.
    J. Biol. Chem. 272:28398-28406(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-81, FUNCTION, MASS SPECTROMETRY, AMIDATION.
    Tissue: Hemocyte.
  2. "Diversity of the penaeidin antimicrobial peptides in two shrimp species."
    Cuthbertson B.J., Shepard E.F., Chapman R.W., Gross P.S.
    Immunogenetics 54:442-445(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Recombinant expression and range of activity of penaeidins, antimicrobial peptides from penaeid shrimp."
    Destoumieux D., Bulet P., Strub J.-M., van Dorsselaer A., Bachere E.
    Eur. J. Biochem. 266:335-346(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-82, PROTEIN SEQUENCE OF 20-31, FUNCTION.
  4. "Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge."
    Destoumieux D., Munoz M., Cosseau C., Rodriguez J., Bulet P., Comps M., Bachere E.
    J. Cell Sci. 113:461-469(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHITIN-BINDING PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Tissue: Hemocyte.
  5. "Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda)."
    Destoumieux D., Munoz M., Bulet P., Bachere E.
    Cell. Mol. Life Sci. 57:1260-1271(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "Solution structure of the recombinant penaeidin-3, a shrimp antimicrobial peptide."
    Yang Y., Poncet J., Garnier J., Zatylny C., Bachere E., Aumelas A.
    J. Biol. Chem. 278:36859-36867(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiPEN3A_LITVA
AccessioniPrimary (citable) accession number: P81058
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: October 14, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.