Reviewed, UniProtKB/Swiss-Prot P81055 (PLMP_PLEOS)

Names and origin

Protein names

Recommended name:
    Peptidyl-Lys metalloendopeptidase
      Short name=MEP
    EC=3.4.24.20
Alternative name(s):
    PoMEP

Gene names

Name:

MEP

Organism

Pleurotus ostreatus (Oyster mushroom) (White-rot fungus)

Taxonomic identifier

5322 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Pleurotaceae › Pleurotus

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage in proteins: -Xaa-|-Lys-(in which Xaa may be Pro).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	Inhibited by chelating agents such as EDTA and 1,10-phenanthroline. Ref.2 Ref.4
Subcellular location	Secreted. Note: Binds strongly to beta-1,3-glucan and chitin, major polysaccharides constituting the fungal cell wall By similarity.
Sequence similarities	Belongs to the peptidase M35 family.
Mass spectrometry	Molecular mass is 17927 Da from positions 1 - 168. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 168	168	Peptidyl-Lys metalloendopeptidase		PRO_0000078237
Sites
	Active site	119	1	By similarity
	Metal binding	118	1	Zinc; catalytic By similarity
	Metal binding	122	1	Zinc; catalytic By similarity
	Metal binding	131	1	Zinc; catalytic By similarity
	Site	134	1	Transition state stabilizer By similarity
Amino acid modifications
	Disulfide bond	6 ↔ 76		By similarity
	Disulfide bond	78 ↔ 98		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81055-1 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 78E5556CB1566547	FASTA	168	17,925
	10 20 30 40 50 60 ATFVGCSATR QTQLNAAASQ AQTYAANALS YLNSHTSSTT RYTTWFGTFV TSRYNTVLSH 70 80 90 100 110 120 FSSISSNTFS SYTFDCTCSD SGTYAFVNPS NFGYVTLCGA FWNAPVAGTD SRGGTLIHES 130 140 150 160 SHFTRNGGTD DHVYGQAGAQ SLARSNPAQA IDNADSHEYF AENNPALA

References

[1]	"Amino acid sequences of metalloendopeptidases specific for acyl-lysine bonds from Grifola frondosa and Pleurotus ostreatus fruiting bodies." Nonaka T., Dohmae N., Hashimoto Y., Takio K. J. Biol. Chem. 272:30032-30039(1997) [PubMed: 9374478] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY.
[2]	"Purification and characterization of intracellular proteinases in Pleurotus ostreatus fruiting bodies." Dohmae N., Hayashi K., Miki K., Tsumuraya Y., Hashimoto Y. Biosci. Biotechnol. Biochem. 59:2074-2080(1995) [PubMed: 8541645] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-11, ENZYME REGULATION.
[3]	"Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies." Nonaka T., Hashimoto Y., Takio K. J. Biochem. 124:157-162(1998) [PubMed: 9644258] [Abstract] Cited for: SUBSTRATE SPECIFICITY.
[4]	"Purification and partial characterization of a fibrinolytic protease in Pleurotus ostreatus." Choi H.-S., Shin H.-H. Mycologia 90:674-679(1998) [Agricola: IND21804865] Cited for: ENZYME REGULATION, ZINC-BINDING.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1G12 based on UniProtKB P81054.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.24.20. 16687.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLMP_PLEOS

Accession

Primary (citable) accession number: P81055

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)