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P80969

- THT10_TOBAC

UniProt

P80969 - THT10_TOBAC

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Protein

Tyramine N-feruloyltransferase 10/30

Gene

THT10

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Synthesizes amides which are involved in stress response in the cell wall. Catalyzes the synthesis of hydroxycinnamic acid amides from hydroxycinnamoyl-CoA thioesters and various hydroxyphenylethylamines such as 4-coumaroyl-CoA and sinapoyl-CoA.

Catalytic activityi

Feruloyl-CoA + tyramine = CoA + N-feruloyltyramine.

Enzyme regulationi

Inhibited by (2-hydroxyphenyl)amino sulfinyl acetic acid 1,1-dimethylethyl ester, by DEPC and by N-ethylmaleimide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei157 – 1571Important for catalytic activity
Sitei160 – 1601Important for catalytic activity
Sitei162 – 1621Important for catalytic activity

GO - Molecular functioni

  1. N-acetyltransferase activity Source: InterPro
  2. tyramine N-feruloyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Tyramine N-feruloyltransferase 10/30 (EC:2.3.1.110)
Alternative name(s):
Hydroxycinnamoyl-CoA: tyramine N-hydroxycinnamoyltransferase
Gene namesi
Name:THT10
Synonyms:THT30
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Tyramine N-feruloyltransferase 10/30PRO_0000074601Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP80969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 222151N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 4517Important in binding site and for catalytic activitySequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the acetyltransferase family.Curated
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80969-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATTNNKNLT ITEKVYVRVR LANEADISHI YKLFYQIHEY HNYTHLYKAT
60 70 80 90 100
ESSLCDLLFK ANPNPLFYGP SVLLLEVSPT PFENTKKDEK FKPVLKTFDL
110 120 130 140 150
RATVEDKEAE EFKSKSCGDE KEDVFIAGYA FFYANYSCFY DKAGIYFESL
160 170 180 190 200
YFRESYRKLG MGGLLFGTVA SIAANNGFAS VEGIVAVWNK KSYDFYVNMG
210 220
VEIFDEFRYG KLVGDALQKY ADKEKV
Length:226
Mass (Da):25,976
Last modified:November 16, 2001 - v2
Checksum:i612930DFC58A0493
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 325SHIYK → HEYHN AA sequence (PubMed:9288939)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005062 mRNA. Translation: CAB55503.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005062 mRNA. Translation: CAB55503.1 .

3D structure databases

ProteinModelPortali P80969.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.630.30. 2 hits.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view ]
Pfami PF00583. Acetyltransf_1. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 2 hits.
PROSITEi PS51186. GNAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification and characterization of cDNA clones encoding hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyltransferase from tobacco."
    Farmer M.J., Czernic P., Michael A., Negrel J.
    Eur. J. Biochem. 263:686-694(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Bottom Special.
    Tissue: Leaf.
  2. "Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures."
    Negrel J., Javelle F.
    Eur. J. Biochem. 247:1127-1135(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-60 AND 67-71.
    Strain: cv. Xanthi.

Entry informationi

Entry nameiTHT10_TOBAC
AccessioniPrimary (citable) accession number: P80969
Secondary accession number(s): Q9SMB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 16, 2001
Last modified: October 29, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3