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P80969

- THT10_TOBAC

UniProt

P80969 - THT10_TOBAC

Protein

Tyramine N-feruloyltransferase 10/30

Gene

THT10

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 2 (16 Nov 2001)
      Previous versions | rss
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    Functioni

    Synthesizes amides which are involved in stress response in the cell wall. Catalyzes the synthesis of hydroxycinnamic acid amides from hydroxycinnamoyl-CoA thioesters and various hydroxyphenylethylamines such as 4-coumaroyl-CoA and sinapoyl-CoA.

    Catalytic activityi

    Feruloyl-CoA + tyramine = CoA + N-feruloyltyramine.

    Enzyme regulationi

    Inhibited by (2-hydroxyphenyl)amino sulfinyl acetic acid 1,1-dimethylethyl ester, by DEPC and by N-ethylmaleimide.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei157 – 1571Important for catalytic activity
    Sitei160 – 1601Important for catalytic activity
    Sitei162 – 1621Important for catalytic activity

    GO - Molecular functioni

    1. N-acetyltransferase activity Source: InterPro
    2. tyramine N-feruloyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyramine N-feruloyltransferase 10/30 (EC:2.3.1.110)
    Alternative name(s):
    Hydroxycinnamoyl-CoA: tyramine N-hydroxycinnamoyltransferase
    Gene namesi
    Name:THT10
    Synonyms:THT30
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 226226Tyramine N-feruloyltransferase 10/30PRO_0000074601Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP80969.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 222151N-acetyltransferasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni29 – 4517Important in binding site and for catalytic activitySequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the acetyltransferase family.Curated
    Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.630.30. 2 hits.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    PfamiPF00583. Acetyltransf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 2 hits.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P80969-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTNNKNLT ITEKVYVRVR LANEADISHI YKLFYQIHEY HNYTHLYKAT    50
    ESSLCDLLFK ANPNPLFYGP SVLLLEVSPT PFENTKKDEK FKPVLKTFDL 100
    RATVEDKEAE EFKSKSCGDE KEDVFIAGYA FFYANYSCFY DKAGIYFESL 150
    YFRESYRKLG MGGLLFGTVA SIAANNGFAS VEGIVAVWNK KSYDFYVNMG 200
    VEIFDEFRYG KLVGDALQKY ADKEKV 226
    Length:226
    Mass (Da):25,976
    Last modified:November 16, 2001 - v2
    Checksum:i612930DFC58A0493
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 325SHIYK → HEYHN AA sequence (PubMed:9288939)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005062 mRNA. Translation: CAB55503.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005062 mRNA. Translation: CAB55503.1 .

    3D structure databases

    ProteinModelPortali P80969.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.630.30. 2 hits.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view ]
    Pfami PF00583. Acetyltransf_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 2 hits.
    PROSITEi PS51186. GNAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of cDNA clones encoding hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyltransferase from tobacco."
      Farmer M.J., Czernic P., Michael A., Negrel J.
      Eur. J. Biochem. 263:686-694(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Bottom Special.
      Tissue: Leaf.
    2. "Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures."
      Negrel J., Javelle F.
      Eur. J. Biochem. 247:1127-1135(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-60 AND 67-71.
      Strain: cv. Xanthi.

    Entry informationi

    Entry nameiTHT10_TOBAC
    AccessioniPrimary (citable) accession number: P80969
    Secondary accession number(s): Q9SMB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 16, 2001
    Last modified: October 1, 2014
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3