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P80969 (THT10_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyramine N-feruloyltransferase 10/30

EC=2.3.1.110
Alternative name(s):
Hydroxycinnamoyl-CoA: tyramine N-hydroxycinnamoyltransferase
Gene names
Name:THT10
Synonyms:THT30
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Synthesizes amides which are involved in stress response in the cell wall. Catalyzes the synthesis of hydroxycinnamic acid amides from hydroxycinnamoyl-CoA thioesters and various hydroxyphenylethylamines such as 4-coumaroyl-CoA and sinapoyl-CoA.

Catalytic activity

Feruloyl-CoA + tyramine = CoA + N-feruloyltyramine.

Enzyme regulation

Inhibited by (2-hydroxyphenyl)amino sulfinyl acetic acid 1,1-dimethylethyl ester, by DEPC and by N-ethylmaleimide.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the acetyltransferase family.

Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionN-acetyltransferase activity

Inferred from electronic annotation. Source: InterPro

tyramine N-feruloyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226Tyramine N-feruloyltransferase 10/30
PRO_0000074601

Regions

Domain72 – 222151N-acetyltransferase
Region29 – 4517Important in binding site and for catalytic activity Potential

Sites

Site1571Important for catalytic activity
Site1601Important for catalytic activity
Site1621Important for catalytic activity

Experimental info

Sequence conflict28 – 325SHIYK → HEYHN AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P80969 [UniParc].

Last modified November 16, 2001. Version 2.
Checksum: 612930DFC58A0493

FASTA22625,976
        10         20         30         40         50         60 
MATTNNKNLT ITEKVYVRVR LANEADISHI YKLFYQIHEY HNYTHLYKAT ESSLCDLLFK 

        70         80         90        100        110        120 
ANPNPLFYGP SVLLLEVSPT PFENTKKDEK FKPVLKTFDL RATVEDKEAE EFKSKSCGDE 

       130        140        150        160        170        180 
KEDVFIAGYA FFYANYSCFY DKAGIYFESL YFRESYRKLG MGGLLFGTVA SIAANNGFAS 

       190        200        210        220 
VEGIVAVWNK KSYDFYVNMG VEIFDEFRYG KLVGDALQKY ADKEKV 

« Hide

References

[1]"Identification and characterization of cDNA clones encoding hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyltransferase from tobacco."
Farmer M.J., Czernic P., Michael A., Negrel J.
Eur. J. Biochem. 263:686-694(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Bottom Special.
Tissue: Leaf.
[2]"Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures."
Negrel J., Javelle F.
Eur. J. Biochem. 247:1127-1135(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-60 AND 67-71.
Strain: cv. Xanthi.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ005062 mRNA. Translation: CAB55503.1.

3D structure databases

ProteinModelPortalP80969.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.630.30. 2 hits.
InterProIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMSSF55729. SSF55729. 2 hits.
PROSITEPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHT10_TOBAC
AccessionPrimary (citable) accession number: P80969
Secondary accession number(s): Q9SMB5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 16, 2001
Last modified: April 16, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families