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P80969

- THT10_TOBAC

UniProt

P80969 - THT10_TOBAC

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Protein
Tyramine N-feruloyltransferase 10/30
Gene
THT10, THT30
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Synthesizes amides which are involved in stress response in the cell wall. Catalyzes the synthesis of hydroxycinnamic acid amides from hydroxycinnamoyl-CoA thioesters and various hydroxyphenylethylamines such as 4-coumaroyl-CoA and sinapoyl-CoA.

Catalytic activityi

Feruloyl-CoA + tyramine = CoA + N-feruloyltyramine.

Enzyme regulationi

Inhibited by (2-hydroxyphenyl)amino sulfinyl acetic acid 1,1-dimethylethyl ester, by DEPC and by N-ethylmaleimide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei157 – 1571Important for catalytic activity
Sitei160 – 1601Important for catalytic activity
Sitei162 – 1621Important for catalytic activity

GO - Molecular functioni

  1. N-acetyltransferase activity Source: InterPro
  2. tyramine N-feruloyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Tyramine N-feruloyltransferase 10/30 (EC:2.3.1.110)
Alternative name(s):
Hydroxycinnamoyl-CoA: tyramine N-hydroxycinnamoyltransferase
Gene namesi
Name:THT10
Synonyms:THT30
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

Cytoplasm Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Tyramine N-feruloyltransferase 10/30
PRO_0000074601Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP80969.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 222151N-acetyltransferase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 4517Important in binding site and for catalytic activity Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the acetyltransferase family.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80969-1 [UniParc]FASTAAdd to Basket

« Hide

MATTNNKNLT ITEKVYVRVR LANEADISHI YKLFYQIHEY HNYTHLYKAT    50
ESSLCDLLFK ANPNPLFYGP SVLLLEVSPT PFENTKKDEK FKPVLKTFDL 100
RATVEDKEAE EFKSKSCGDE KEDVFIAGYA FFYANYSCFY DKAGIYFESL 150
YFRESYRKLG MGGLLFGTVA SIAANNGFAS VEGIVAVWNK KSYDFYVNMG 200
VEIFDEFRYG KLVGDALQKY ADKEKV 226
Length:226
Mass (Da):25,976
Last modified:November 16, 2001 - v2
Checksum:i612930DFC58A0493
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 325SHIYK → HEYHN AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005062 mRNA. Translation: CAB55503.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005062 mRNA. Translation: CAB55503.1 .

3D structure databases

ProteinModelPortali P80969.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.630.30. 2 hits.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view ]
Pfami PF00583. Acetyltransf_1. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 2 hits.
PROSITEi PS51186. GNAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification and characterization of cDNA clones encoding hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyltransferase from tobacco."
    Farmer M.J., Czernic P., Michael A., Negrel J.
    Eur. J. Biochem. 263:686-694(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Bottom Special.
    Tissue: Leaf.
  2. "Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures."
    Negrel J., Javelle F.
    Eur. J. Biochem. 247:1127-1135(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-60 AND 67-71.
    Strain: cv. Xanthi.

Entry informationi

Entry nameiTHT10_TOBAC
AccessioniPrimary (citable) accession number: P80969
Secondary accession number(s): Q9SMB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 16, 2001
Last modified: April 16, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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