P80969 (THT10_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyramine N-feruloyltransferase 10/30
EC=2.3.1.110

Alternative name(s):
Hydroxycinnamoyl-CoA: tyramine N-hydroxycinnamoyltransferase

Gene names

Name:	THT10
Synonyms:	THT30

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Synthesizes amides which are involved in stress response in the cell wall. Catalyzes the synthesis of hydroxycinnamic acid amides from hydroxycinnamoyl-CoA thioesters and various hydroxyphenylethylamines such as 4-coumaroyl-CoA and sinapoyl-CoA.
Catalytic activity	Feruloyl-CoA + tyramine = CoA + N-feruloyltyramine.
Enzyme regulation	Inhibited by (2-hydroxyphenyl)amino sulfinyl acetic acid 1,1-dimethylethyl ester, by DEPC and by N-ethylmaleimide.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetyltransferase activity Inferred from electronic annotation. Source: InterPro tyramine N-feruloyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Tyramine N-feruloyltransferase 10/30		PRO_0000074601
Regions
	Domain	72 – 222	151	N-acetyltransferase
	Region	29 – 45	17	Important in binding site and for catalytic activity Potential
Sites
	Site	157	1	Important for catalytic activity
	Site	160	1	Important for catalytic activity
	Site	162	1	Important for catalytic activity
Experimental info
	Sequence conflict	28 – 32	5	SHIYK → HEYHN AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80969 [UniParc]. Last modified November 16, 2001. Version 2. Checksum: 612930DFC58A0493	FASTA	226	25,976
	10 20 30 40 50 60 MATTNNKNLT ITEKVYVRVR LANEADISHI YKLFYQIHEY HNYTHLYKAT ESSLCDLLFK 70 80 90 100 110 120 ANPNPLFYGP SVLLLEVSPT PFENTKKDEK FKPVLKTFDL RATVEDKEAE EFKSKSCGDE 130 140 150 160 170 180 KEDVFIAGYA FFYANYSCFY DKAGIYFESL YFRESYRKLG MGGLLFGTVA SIAANNGFAS 190 200 210 220 VEGIVAVWNK KSYDFYVNMG VEIFDEFRYG KLVGDALQKY ADKEKV

References

[1]	"Identification and characterization of cDNA clones encoding hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyltransferase from tobacco." Farmer M.J., Czernic P., Michael A., Negrel J. Eur. J. Biochem. 263:686-694(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bottom Special. Tissue: Leaf.
[2]	"Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures." Negrel J., Javelle F. Eur. J. Biochem. 247:1127-1135(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-60 AND 67-71. Strain: cv. Xanthi.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005062 mRNA. Translation: CAB55503.1.
3D structure databases
ProteinModelPortal	P80969.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.630.30. 2 hits.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THT10_TOBAC

Accession

Primary (citable) accession number: P80969
Secondary accession number(s): Q9SMB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 16, 2001
Last modified:	April 3, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program