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Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
Binding sitei33 – 331SubstrateBy similarity
Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
Binding sitei98 – 981SubstrateBy similarity

GO - Molecular functioni

  1. chemoattractant activity Source: Ensembl
  2. dopachrome isomerase activity Source: UniProtKB
  3. phenylpyruvate tautomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell aging Source: Ensembl
  2. cell proliferation Source: Ensembl
  3. cell surface receptor signaling pathway Source: Ensembl
  4. DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  5. inflammatory response Source: UniProtKB-KW
  6. innate immune response Source: UniProtKB-KW
  7. negative regulation of cell aging Source: Ensembl
  8. negative regulation of cell cycle arrest Source: Ensembl
  9. negative regulation of cellular protein metabolic process Source: Ensembl
  10. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  11. negative regulation of gene expression Source: Ensembl
  12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
  13. negative regulation of mature B cell apoptotic process Source: Ensembl
  14. negative regulation of myeloid cell apoptotic process Source: Ensembl
  15. positive regulation of arachidonic acid secretion Source: Ensembl
  16. positive regulation of B cell proliferation Source: Ensembl
  17. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: Ensembl
  18. positive regulation of cytokine secretion Source: Ensembl
  19. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  20. positive regulation of fibroblast proliferation Source: Ensembl
  21. positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
  22. positive regulation of MAP kinase activity Source: Ensembl
  23. positive regulation of myeloid leukocyte cytokine production involved in immune response Source: Ensembl
  24. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
  25. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  26. positive regulation of prostaglandin secretion involved in immune response Source: Ensembl
  27. positive regulation of protein kinase A signaling Source: Ensembl
  28. prostaglandin biosynthetic process Source: Ensembl
  29. protein homotrimerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
Glycosylation-inhibiting factor
Short name:
GIF
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:MIF
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 14

Subcellular locationi

Secreted By similarity. Cytoplasm By similarity
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens (By similarity).By similarity

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. cytoplasm Source: UniProtKB-SubCell
  3. extracellular space Source: UniProtKB-KW
  4. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781N6-acetyllysine; alternateBy similarity
Modified residuei78 – 781N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP80928.
PRIDEiP80928.

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP80928.
SMRiP80928. Positions 1-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiNOG08790.
GeneTreeiENSGT00730000111101.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
InParanoidiP80928.
KOiK07253.
OMAiMGKPAQY.
OrthoDBiEOG7GXPDN.
TreeFamiTF313853.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMFVVNTNV PRASVPDGFL SELTQQLVQA MGKPAQYIAV HVVPDQLMAF
60 70 80 90 100
GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRIYINYY
110
DMNAANVGWN GSTFA
Length:115
Mass (Da):12,451
Last modified:January 23, 2007 - v3
Checksum:i927098BA62A1A879
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ989235 mRNA. Translation: ABI95381.1.
AF176246 mRNA. Translation: AAD50507.1.
PIRiPC4367.
RefSeqiNP_001070681.1. NM_001077213.2.
UniGeneiSsc.551.

Genome annotation databases

EnsembliENSSSCT00000011028; ENSSSCP00000010740; ENSSSCG00000010067.
GeneIDi397412.
KEGGissc:397412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ989235 mRNA. Translation: ABI95381.1.
AF176246 mRNA. Translation: AAD50507.1.
PIRiPC4367.
RefSeqiNP_001070681.1. NM_001077213.2.
UniGeneiSsc.551.

3D structure databases

ProteinModelPortaliP80928.
SMRiP80928. Positions 1-115.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP80928.
PRIDEiP80928.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000011028; ENSSSCP00000010740; ENSSSCG00000010067.
GeneIDi397412.
KEGGissc:397412.

Organism-specific databases

CTDi4282.

Phylogenomic databases

eggNOGiNOG08790.
GeneTreeiENSGT00730000111101.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
InParanoidiP80928.
KOiK07253.
OMAiMGKPAQY.
OrthoDBiEOG7GXPDN.
TreeFamiTF313853.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of porcine macrophage migration inhibitory factor (MIF)."
    Chen Y., Chen X., Long Q., Yang Z.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Pituitary function in the acute phase response in domestic farm animals: cytokines, prostaglandins, and secretion of ACTH."
    Abraham E.J., Morris-Hardeman J.N., Swenson L.M., Knoppel E.L., Ramanathan B., Wright K.J., Grieger D.M., Minton J.E.
    Domest. Anim. Endocrinol. 15:389-396(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-111.
  3. Riviere S., Bouet F., Menez A., Galat A.
    Submitted (MAR-1997) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-21.

Entry informationi

Entry nameiMIF_PIG
AccessioniPrimary (citable) accession number: P80928
Secondary accession number(s): Q069I4, Q9TUM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.