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P80928

- MIF_PIG

UniProt

P80928 - MIF_PIG

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Protein
Macrophage migration inhibitory factor
Gene
MIF
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogen By similarity
Binding sitei33 – 331Substrate By similarity
Binding sitei65 – 651Substrate; via amide nitrogen By similarity
Binding sitei98 – 981Substrate By similarity

GO - Molecular functioni

  1. chemoattractant activity Source: Ensembl
  2. dopachrome isomerase activity Source: UniProtKB
  3. phenylpyruvate tautomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  2. cell aging Source: Ensembl
  3. cell proliferation Source: Ensembl
  4. cell surface receptor signaling pathway Source: Ensembl
  5. inflammatory response Source: UniProtKB-KW
  6. innate immune response Source: UniProtKB-KW
  7. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  8. negative regulation of cell aging Source: Ensembl
  9. negative regulation of cell cycle arrest Source: Ensembl
  10. negative regulation of cellular protein metabolic process Source: Ensembl
  11. negative regulation of gene expression Source: Ensembl
  12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
  13. negative regulation of mature B cell apoptotic process Source: Ensembl
  14. negative regulation of myeloid cell apoptotic process Source: Ensembl
  15. positive regulation of B cell proliferation Source: Ensembl
  16. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  17. positive regulation of MAP kinase activity Source: Ensembl
  18. positive regulation of arachidonic acid secretion Source: Ensembl
  19. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: Ensembl
  20. positive regulation of cytokine secretion Source: Ensembl
  21. positive regulation of fibroblast proliferation Source: Ensembl
  22. positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
  23. positive regulation of myeloid leukocyte cytokine production involved in immune response Source: Ensembl
  24. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
  25. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  26. positive regulation of prostaglandin secretion involved in immune response Source: Ensembl
  27. positive regulation of protein kinase A signaling Source: Ensembl
  28. prostaglandin biosynthetic process Source: Ensembl
  29. protein homotrimerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
Glycosylation-inhibiting factor
Short name:
GIF
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:MIF
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 14

Subcellular locationi

Secreted By similarity. Cytoplasm By similarity
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. cytoplasm Source: UniProtKB-SubCell
  3. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 115114Macrophage migration inhibitory factor
PRO_0000158067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781N6-acetyllysine; alternate By similarity
Modified residuei78 – 781N6-succinyllysine; alternate By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP80928.
PRIDEiP80928.

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.

Structurei

3D structure databases

ProteinModelPortaliP80928.
SMRiP80928. Positions 1-115.

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.

Phylogenomic databases

eggNOGiNOG08790.
GeneTreeiENSGT00730000111101.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
KOiK07253.
OMAiKPERYVM.
OrthoDBiEOG7GXPDN.
TreeFamiTF313853.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80928-1 [UniParc]FASTAAdd to Basket

« Hide

MPMFVVNTNV PRASVPDGFL SELTQQLVQA MGKPAQYIAV HVVPDQLMAF    50
GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRIYINYY 100
DMNAANVGWN GSTFA 115
Length:115
Mass (Da):12,451
Last modified:January 23, 2007 - v3
Checksum:i927098BA62A1A879
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ989235 mRNA. Translation: ABI95381.1.
AF176246 mRNA. Translation: AAD50507.1.
PIRiPC4367.
RefSeqiNP_001070681.1. NM_001077213.2.
UniGeneiSsc.551.

Genome annotation databases

EnsembliENSSSCT00000011028; ENSSSCP00000010740; ENSSSCG00000010067.
GeneIDi397412.
KEGGissc:397412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ989235 mRNA. Translation: ABI95381.1 .
AF176246 mRNA. Translation: AAD50507.1 .
PIRi PC4367.
RefSeqi NP_001070681.1. NM_001077213.2.
UniGenei Ssc.551.

3D structure databases

ProteinModelPortali P80928.
SMRi P80928. Positions 1-115.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P80928.
PRIDEi P80928.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000011028 ; ENSSSCP00000010740 ; ENSSSCG00000010067 .
GeneIDi 397412.
KEGGi ssc:397412.

Organism-specific databases

CTDi 4282.

Phylogenomic databases

eggNOGi NOG08790.
GeneTreei ENSGT00730000111101.
HOGENOMi HOG000112325.
HOVERGENi HBG003240.
KOi K07253.
OMAi KPERYVM.
OrthoDBi EOG7GXPDN.
TreeFami TF313853.

Family and domain databases

InterProi IPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view ]
PANTHERi PTHR11954. PTHR11954. 1 hit.
Pfami PF01187. MIF. 1 hit.
[Graphical view ]
ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55331. SSF55331. 1 hit.
PROSITEi PS01158. MIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of porcine macrophage migration inhibitory factor (MIF)."
    Chen Y., Chen X., Long Q., Yang Z.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Pituitary function in the acute phase response in domestic farm animals: cytokines, prostaglandins, and secretion of ACTH."
    Abraham E.J., Morris-Hardeman J.N., Swenson L.M., Knoppel E.L., Ramanathan B., Wright K.J., Grieger D.M., Minton J.E.
    Domest. Anim. Endocrinol. 15:389-396(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-111.
  3. Riviere S., Bouet F., Menez A., Galat A.
    Submitted (MAR-1997) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-21.

Entry informationi

Entry nameiMIF_PIG
AccessioniPrimary (citable) accession number: P80928
Secondary accession number(s): Q069I4, Q9TUM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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