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Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Proton acceptor; via imino nitrogenBy similarity1
Binding sitei33SubstrateBy similarity1
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei98SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Isomerase
Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-SSC-202733 Cell surface interactions at the vascular wall
R-SSC-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
Glycosylation-inhibiting factor
Short name:
GIF
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:MIF
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001580672 – 115Macrophage migration inhibitory factorAdd BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78N6-acetyllysine; alternateBy similarity1
Modified residuei78N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP80928
PeptideAtlasiP80928
PRIDEiP80928

Expressioni

Gene expression databases

BgeeiENSSSCG00000010067
GenevisibleiP80928 SS

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010740

Structurei

3D structure databases

ProteinModelPortaliP80928
SMRiP80928
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiKOG1759 Eukaryota
ENOG41122MF LUCA
GeneTreeiENSGT00910000144267
HOGENOMiHOG000112325
HOVERGENiHBG003240
InParanoidiP80928
KOiK07253
OMAiPDRIYIN
OrthoDBiEOG091G0ZNJ
TreeFamiTF313853

Family and domain databases

Gene3Di3.30.429.10, 1 hit
InterProiView protein in InterPro
IPR001398 Macrophage_inhib_fac
IPR019829 Macrophage_inhib_fac_CS
IPR014347 Tautomerase/MIF_sf
PANTHERiPTHR11954 PTHR11954, 1 hit
PfamiView protein in Pfam
PF01187 MIF, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004816 Macrophage_inhib_fac, 1 hit
SUPFAMiSSF55331 SSF55331, 1 hit
PROSITEiView protein in PROSITE
PS01158 MIF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMFVVNTNV PRASVPDGFL SELTQQLVQA MGKPAQYIAV HVVPDQLMAF
60 70 80 90 100
GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRIYINYY
110
DMNAANVGWN GSTFA
Length:115
Mass (Da):12,451
Last modified:January 23, 2007 - v3
Checksum:i927098BA62A1A879
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ989235 mRNA Translation: ABI95381.1
AF176246 mRNA Translation: AAD50507.1
PIRiPC4367
RefSeqiNP_001070681.1, NM_001077213.2
UniGeneiSsc.551

Genome annotation databases

EnsembliENSSSCT00000011028; ENSSSCP00000010740; ENSSSCG00000010067
GeneIDi397412
KEGGissc:397412

Similar proteinsi

Entry informationi

Entry nameiMIF_PIG
AccessioniPrimary (citable) accession number: P80928
Secondary accession number(s): Q069I4, Q9TUM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 114 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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