Reviewed, UniProtKB/Swiss-Prot P80924 (PHLC_STAIN)

Names and origin

Protein names	Recommended name:     Sphingomyelinase C     EC=3.1.4.12 Alternative name(s):     Neutral sphingomyelinase       Short name=nSMase     Beta-toxin     Beta-hemolysin
Organism	Staphylococcus intermedius
Taxonomic identifier	1285 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	19 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required, with sphingomyelinase, to effect target cell lysis (hemolysis).
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Cofactor	Magnesium.
Subunit structure	Monomer.
Miscellaneous	It has a high specificity for sphingomyelin, hydrolyzes lysophosphatidylcholine at a much lower rate, but has no activity towards phosphatidylcholine, phosphatidylethanolamine, or phosphatidylserine.
Sequence similarities	Belongs to the neutral sphingomyelinase family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0-7.5.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Ligand	Magnesium
Molecular function	Hydrolase Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis by symbiont of host erythrocytes Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›19	›19	Sphingomyelinase C		PRO_0000075696
Experimental info
	Non-terminal residue	19	1

Sequences

	Sequence		Length	Mass (Da)
	P80924-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: A50753FCF500F80E	FASTA	19	2,089
	10 ASGENQAELK LATHNVYML

References

[1]	"Comparison of the beta-toxins from Staphylococcus aureus and Staphylococcus intermedius." Dziewanowska K., Edwards V.M., Deringer J.R., Bohach G.A., Guerra D.J. Arch. Biochem. Biophys. 335:102-108(1996) [PubMed: 8914839] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 94-072594.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.4.12. 290681.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PHLC_STAIN

Accession

Primary (citable) accession number: P80924

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 5, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)