P80910 (IORA_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indolepyruvate oxidoreductase subunit iorA
Short name=IOR
EC=1.2.7.8

Alternative name(s):
Indolepyruvate ferredoxin oxidoreductase subunit alpha

Gene names

Name:	iorA
Ordered Locus Names:	MTBMA_c04220

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.
Catalytic activity	(Indol-3-yl)pyruvate + CoA + 2 oxidized ferredoxin = S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 1 4Fe-4S cluster.
Subunit structure	Heterodimer of the iorA and iorB subunits. Ref.2
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.
Biophysicochemical properties	pH dependence: Optimum pH is 10.0. Ref.2 Temperature dependence: Optimum temperature is 80 degrees Celsius.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro indolepyruvate ferredoxin oxidoreductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Indolepyruvate oxidoreductase subunit iorA		PRO_0000099927
Regions
	Domain	559 – 590	32	4Fe-4S ferredoxin-type 1
	Domain	588 – 617	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	597	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	600	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	603	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	607	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	11	1	R → V AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80910 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: E1D0F3A9B2B406AB	FASTA	618	67,098
	10 20 30 40 50 60 MELDDILDAG RGDRLFLLGN EAAVRAAIES GVGVASTYPG TPSSEIGNVL SKIAKRAGIY 70 80 90 100 110 120 FEFSINEKVA LEVAAAAAAS GVRSFTFMKH VGLNVASDSF MSVAYTGVRA GMVVLSADDP 130 140 150 160 170 180 SMFSSQNEQD NRHYARLAWV PLLEPSNPQE ILEYMNHAFE LSEEYRIPVL LRTTTRVSHM 190 200 210 220 230 240 RGVVEAGERR AEPVKGFFRK NPEQFVPVPA TARVMRRELV EKMKKLKRVA DTSELNRVLN 250 260 270 280 290 300 EDSESDLGII ASGGAFNYVY DALQTLGLDV PVLKLGFTYP FPAGLVAEFL SGLEGVLVVE 310 320 330 340 350 360 EVDSVMEKEV LAVATSEGLD VGVHGKLDGT LPEIYEYSED IVRRAISGLT GIKSHEKGIE 370 380 390 400 410 420 APELPERPPA LCPGCPHRAM YYSVRRAASE LGIEGEDLIF PTDIGCYTLG IEPPYSAADY 430 440 450 460 470 480 LLSMGSSVGT ACGFSAATSQ RIVSFIGDST FFHAGIPPLI NAVHNRQRFV LVILDNRTTA 490 500 510 520 530 540 MTGGQPHPGL PVDGMGEEAP AISIEDITRA CGVEFVETVN PMNIRRSSET IRRALQHESV 550 560 570 580 590 600 AVVISRYPCM LSEGAVRGRP VRVDEEKCDL CLECLNELAC PAIVEEDGRV FIDPLYCRGC 610 TICLQICPAG AIKPEGKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed: 20802048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum." Tersteegen A., Linder D., Thauer R.K., Hedderich R. Eur. J. Biochem. 244:862-868(1997) [PubMed: 9108258] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-27, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001710 Genomic DNA. Translation: ADL58023.1.
RefSeq	YP_003849336.1. NC_014408.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9704128.
GenomeReviews	Gene locus MTBMA_c04220 in contig CP001710_GR.
KEGG	mmg:MTBMA_c04220.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VADDPSM.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017721. Indolepyruvate_Fd_OxRdtase_asu. IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K00179.
Pfam	PF01855. POR_N. 1 hit. PF02775. TPP_enzyme_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR03336. IOR_alpha. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IORA_METTM

Accession

Primary (citable) accession number: P80910
Secondary accession number(s): D9PUX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 30, 2010
Last modified:	November 16, 2011
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program