P80908 (VORB_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketoisovalerate oxidoreductase subunit vorB
Short name=VOR
EC=1.2.7.7

Alternative name(s):
2-oxoisovalerate ferredoxin reductase subunit beta
2-oxoisovalerate oxidoreductase beta chain

Gene names

Name:	vorB
Ordered Locus Names:	MTBMA_c10910

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+.
Subunit structure	Heterotrimer of the vorA, vorB and vorC subunits. Ref.2
Biophysicochemical properties	pH dependence: Optimum pH is 9.7. Ref.2 Temperature dependence: Optimum temperature is 75 degrees Celsius.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 352	351	Ketoisovalerate oxidoreductase subunit vorB		PRO_0000099955

Sequences

	Sequence		Length	Mass (Da)
	P80908 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 6BFE2D51CA971021	FASTA	352	38,549
	10 20 30 40 50 60 MATQMVKGNT AVIIGAMYAG CDCYFGYPIT PASEILHEAS RYFPLVGRKF VQAESEEAAI 70 80 90 100 110 120 NMVYGAAAAG HRVMTASSGP GMSLKQEGIS FLAGAELPAV IVDVMRAGPG LGNIGPEQAD 130 140 150 160 170 180 YNQLVKGGGH GNYRNIVLAP NSVQEMCDLT MDAFELADKY RNPVIILADA VLGQMAEPLR 190 200 210 220 230 240 FPERAVEHRP DTSWAVCGSR ETMKNLVTSI FLDFDELEEF NFYLQEKYAA VEENEVRYEE 250 260 270 280 290 300 YMVEDAEIVL VAYGISSRVA KSAVDTARAD GIKVGLLRPI TLFPFPSERI RELAEGGCTF 310 320 330 340 350 ISVEMSSGQM REDIKMASGC RDVELVNRMG GNLIELRDIL RKIREIAGES ND

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed: 20802048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum." Tersteegen A., Linder D., Thauer R.K., Hedderich R. Eur. J. Biochem. 244:862-868(1997) [PubMed: 9108258] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-36, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001710 Genomic DNA. Translation: ADL58685.1.
RefSeq	YP_003849998.1. NC_014408.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9704799.
GenomeReviews	Gene locus MTBMA_c10910 in contig CP001710_GR.
KEGG	mmg:MTBMA_c10910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CVIVNIM.
Family and domain databases
InterPro	IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K00186.
Pfam	PF01855. POR_N. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VORB_METTM

Accession

Primary (citable) accession number: P80908
Secondary accession number(s): D9PWT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 30, 2010
Last modified:	November 16, 2011
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program