P80897 (RPN2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
EC=2.4.1.119

Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 65-II kDa subunit
Ribophorin II
Short name=RPN-II
Ribophorin-2

Gene names

Name:

RPN2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	36 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the SWP1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›36	›36	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2		PRO_0000058092
Experimental info
	Non-adjacent residues	18 – 19	2
	Non-terminal residue	1	1
	Non-terminal residue	36	1

Sequences

	Sequence		Length	Mass (Da)
	P80897 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2A8D47456E6C132E	FASTA	36	3,927
	10 20 30 NAIFSKKNFE TLSEAFSVFQ TLKYLAILGG VTFLAG

References

[1]	"Purification and characterization of avian oligosaccharyltransferase. Complete amino acid sequence of the 50-kDa subunit." Kumar V., Heinemann F.S., Ozols J. J. Biol. Chem. 269:13451-13457(1994) [PubMed: 8175777] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Oviduct.

Cross-references

Sequence databases
IPI	IPI00577378.
PIR	C54127.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

RPN2_CHICK

Accession

Primary (citable) accession number: P80897

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 21, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program