Reviewed, UniProtKB/Swiss-Prot P80896 (RPN1_CHICK)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
    EC=2.4.1.119
Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 65 kDa subunit
    Ribophorin-1
    Ribophorin I
      Short name=RPN-I
    Oligosaccharyl transferase 65-I kDa subunit

Gene names

Name:

RPN1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	28 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity. Oligomeric complex of three non-identical subunits of 65-I kDa, 65-II kDa and 50 kDa.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the OST1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›28	›28	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1		PRO_0000058091
Experimental info
	Non-adjacent residues	10 – 11	2
	Non-terminal residue	1	1
	Non-terminal residue	28	1

Sequences

	Sequence		Length	Mass (Da)
	P80896-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 5FAC15F4F4FD335A	FASTA	28	3,349
	10 20 VFTHVLQPYP EIRPRFPLVG GWKTHYXV

References

[1]	"Purification and characterization of avian oligosaccharyltransferase. Complete amino acid sequence of the 50-kDa subunit." Kumar V., Heinemann F.S., Ozols J. J. Biol. Chem. 269:13451-13457(1994) [PubMed: 8175777] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Oviduct.

Cross-references

Sequence databases
IPI	IPI00572841.
PIR	B54127.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P80896.
Enzyme and pathway databases
BRENDA	2.4.1.119. 4.
Family and domain databases
InterPro	IPR007676. Ribophorin_I. [Graphical view]
PANTHER	PTHR21049. Ribophorin_I. 1 hit.
Pfam	PF04597. Ribophorin_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPN1_CHICK

Accession

Primary (citable) accession number: P80896

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)