P80894 (GSTA1_ANTST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase EC=2.5.1.18 Alternative name(s): GST class-alpha
Organism	Antechinus stuartii (Brown marsupial mouse)
Taxonomic identifier	9283 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Dasyuromorphia › Dasyuridae › Antechinus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glutathione metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione transferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Glutathione S-transferase		PRO_0000185803
Regions
	Domain	3 – 82	80	GST N-terminal
	Domain	84 – 208	125	GST C-terminal
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P80894 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 03B185BCC44B570F	FASTA	221	25,534
	10 20 30 40 50 60 MAGEQNIKYF NIKGRMEAIR WLLAVAGVEF EEKFFETKEQ LQKLKETVLL FQQVPMVEID 70 80 90 100 110 120 GMKLVQTRAI LHYIAEKYNL LGKDMKEHAQ IIMYSEGTMD LMELIMIYPF LKGEEKKQRL 130 140 150 160 170 180 VEIANKAKGR YFPAFENVLK THGQNFLVGN QLSMADVQLF EAILMVEEKV PDALSGFPLL 190 200 210 220 QAFKTRISNI PTVKTFLAPG SKRKPVPDAK YVEDIIKIFY F

References

[1]	"Characterization of a marsupial glutathione transferase, a class Alpha enzyme from Brown Antechinus (Antechinus stuartii)." Bolton R.M., Curstedt L., Cederlund E., Hjelmqvist L., Mannervik B., Ahokas J.T., Joernvall H. FEBS Lett. 406:216-219(1997) [PubMed: 9109421] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

3D structure databases
ProteinModelPortal	P80894.
SMR	P80894. Positions 2-219.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG053749.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA1_ANTST

Accession

Primary (citable) accession number: P80894

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 27, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program