P80887 (IPYR_CYAPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Inorganic pyrophosphatase EC=3.6.1.1 Alternative name(s): Pyrophosphate phospho-hydrolase Short name=PPase
Organism	Cyanophora paradoxa
Taxonomic identifier	2762 [NCBI]
Taxonomic lineage	Eukaryota › Glaucocystophyceae › Cyanophoraceae › Cyanophora

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. Ref.1
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity. UniProtKB Q6BWA5
Subunit structure	Monomer. Ref.1
Sequence similarities	Belongs to the PPase family.
Biophysicochemical properties	Kinetic parameters: KM=32.4 µM for Mg2-PPi Ref.1
Mass spectrometry	Molecular mass is 26560 Da from positions 1 - ?. Determined by MALDI. Ref.1

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Inorganic pyrophosphatase		PRO_0000253940
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P80887 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 7F4B01D7075A02E9	FASTA	25	2,774
	10 20 XAITAEPVGT PETLEYRVFI QKDGK

References

[1]

"A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes." Gomez-Garcia M.R., Losada M., Serrano A. Biochem. J. 395:211-221(2006) [PubMed: 16313235] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, MASS SPECTROMETRY. Strain: UTEX LB 555 / Pringsheim.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00387. PPASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_CYAPA

Accession

Primary (citable) accession number: P80887

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 17, 2006
Last modified:	May 31, 2011
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)