P80884 (ANAN_ANACO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ananain
EC=3.4.22.31

Gene names

Name:

AN1

Organism

Ananas comosus (Pineapple) (Ananas ananas)

Taxonomic identifier

4615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Bromeliaceae › Ananas

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-|-NHMec, but broader specificity than fruit bromelain.
Enzyme regulation	Strongly inhibited by chicken egg-white cystatin and the active-site-directed inhibitor trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E-64).
Tissue specificity	Stem.
Sequence similarities	Belongs to the peptidase C1 family.
Mass spectrometry	Molecular mass is 23478 Da from positions 123 - 345. Determined by ESI. Ref.2

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 122	98			PRO_0000026400
	Chain	123 – 345	223	Ananain		PRO_0000026401
Sites
	Active site	147	1	By similarity
	Active site	279	1	By similarity
Amino acid modifications
	Disulfide bond	144 ↔ 184		By similarity
	Disulfide bond	178 ↔ 217		By similarity
	Disulfide bond	273 ↔ 325		By similarity
Experimental info
	Sequence conflict	291	1	S → A AA sequence Ref.2
	Sequence conflict	324	1	L → I AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80884 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: FAF2989080174D87	FASTA	345	38,248
	10 20 30 40 50 60 MTSKVQLVFL FLFLCVMWAS PSAASCDEPS DPMMKQFEEW MAEYGRVYKD NDEKMLRFQI 70 80 90 100 110 120 FKNNVNHIET FNNRNGNSYT LGINQFTDMT NNEFVAQYTG LSLPLNIKRE PVVSFDDVDI 130 140 150 160 170 180 SSVPQSIDWR DSGAVTSVKN QGRCGSCWAF ASIATVESIY KIKRGNLVSL SEQQVLDCAV 190 200 210 220 230 240 SYGCKGGWIN KAYSFIISNK GVASAAIYPY KAAKGTCKTN GVPNSAYITR YTYVQRNNER 250 260 270 280 290 300 NMMYAVSNQP IAAALDASGN FQHYKRGVFT GPCGTRLNHA IVIIGYGQDS SGKKFWIVRN 310 320 330 340 SWGAGWGEGG YIRLARDVSS SFGLCGIAMD PLYPTLQSGP SVEVI

References

[1]	"Cloning and expression of ananain gene from pineapple." Robertson C.E., Goodenough P.W. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Smooth Cayenne. Tissue: Stem.
[2]	"Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases." Lee K.L., Albee K.L., Bernasconi R.J., Edmunds T. Biochem. J. 327:199-202(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 123-338, MASS SPECTROMETRY. Tissue: Stem.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ002477 mRNA. Translation: CAA05487.1.
PIR	T07839.
3D structure databases
ProteinModelPortal	P80884.
SMR	P80884. Positions 123-336.
ModBase	Search...
Protein family/group databases
MEROPS	C01.026.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.4.22.31. 333.
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANAN_ANACO

Accession

Primary (citable) accession number: P80884
Secondary accession number(s): O22293

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 27, 2001
Last modified:	April 3, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program