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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi645ZincBy similarity1
Metal bindingi647ZincBy similarity1
Metal bindingi730ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU13180-MONOMER.
MetaCyc:MONOMER-14559.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Superoxide-inducible protein 9
Short name:
SOI9
Gene namesi
Name:metE
Synonyms:metC
Ordered Locus Names:BSU13180
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000986132 – 7625-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST761

Proteomic databases

PaxDbiP80877.
PRIDEiP80877.

Expressioni

Inductioni

By superoxide.

Interactioni

Protein-protein interaction databases

IntActiP80877. 2 interactors.
MINTiMINT-8364775.
STRINGi224308.Bsubs1_010100007301.

Structurei

3D structure databases

ProteinModelPortaliP80877.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DSS. Bacteria.
COG0620. LUCA.
HOGENOMiHOG000246221.
InParanoidiP80877.
KOiK00549.
OMAiRFGWVQS.
PhylomeDBiP80877.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTIKTSNLG FPRIGLNREW KKALEAYWKG STDKDTFLKQ IDELFLSAVK
60 70 80 90 100
TQIDQQIDVV PVSDFTQYDH VLDTAVSFNW IPKRFRHLTD ATDTYFAIAR
110 120 130 140 150
GIKDAVSSEM TKWFNTNYHY IVPEYDESIE FRLTRNKQLE DYRRIKQEYG
160 170 180 190 200
VETKPVIVGP YTFVTLAKGY EPSEAKAIQK RLVPLYVQLL KELEEEGVKW
210 220 230 240 250
VQIDEPALVT ASSEDVRGAK ELFESITSEL SSLNVLLQTY FDSVDAYEEL
260 270 280 290 300
ISYPVQGIGL DFVHDKGRNL EQLKTHGFPT DKVLAAGVID GRNIWKADLE
310 320 330 340 350
ERLDAVLDIL SIAKVDELWI QPSSSLLHVP VAKHPDEHLE KDLLNGLSYA
360 370 380 390 400
KEKLAELTAL KEGLVSGKAA ISEEIQQAKA DIQALKQFAT GANSEQKKEL
410 420 430 440 450
EQLTDKDFKR PIPFEERLAL QNESLGLPLL PTTTIGSFPQ SAEVRSARQK
460 470 480 490 500
WRKAEWSDEQ YQNFINAETK RWIDIQEELE LDVLVHGEFE RTDMVEYFGE
510 520 530 540 550
KLAGFAFTKY AWVQSYGSRC VRPPVIYGDV EFIEPMTVKD TVYAQSLTSK
560 570 580 590 600
HVKGMLTGPV TILNWSFPRN DISRKEIAFQ IGLALRKEVK ALEDAGIQII
610 620 630 640 650
QVDEPALREG LPLKTRDWDE YLTWAAEAFR LTTSSVKNET QIHTHMCYSN
660 670 680 690 700
FEDIVDTIND LDADVITIEH SRSHGGFLDY LKNHPYLKGL GLGVYDIHSP
710 720 730 740 750
RVPSTEEMYN IIVDALAVCP TDRFWVNPDC GLKTRQQEET VAALKNMVEA
760
AKQARAQQTQ LV
Length:762
Mass (Da):86,806
Last modified:June 16, 2009 - v4
Checksum:iBB85B09E6C1179EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15G → D in CAA05597 (Ref. 1) Curated1
Sequence conflicti20W → Q AA sequence (PubMed:9298659).Curated1
Sequence conflicti302R → S in CAA05597 (Ref. 1) Curated1
Sequence conflicti309I → V in CAA05597 (Ref. 1) Curated1
Sequence conflicti328H → D in CAA05597 (Ref. 1) Curated1
Sequence conflicti446S → R in CAA05597 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA. Translation: CAA05597.1.
AL009126 Genomic DNA. Translation: CAB13175.2.
PIRiC69657.
RefSeqiNP_389201.2. NC_000964.3.
WP_003232565.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13175; CAB13175; BSU13180.
GeneIDi936480.
KEGGibsu:BSU13180.
PATRICi18974395. VBIBacSub10457_1390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA. Translation: CAA05597.1.
AL009126 Genomic DNA. Translation: CAB13175.2.
PIRiC69657.
RefSeqiNP_389201.2. NC_000964.3.
WP_003232565.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP80877.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP80877. 2 interactors.
MINTiMINT-8364775.
STRINGi224308.Bsubs1_010100007301.

Proteomic databases

PaxDbiP80877.
PRIDEiP80877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13175; CAB13175; BSU13180.
GeneIDi936480.
KEGGibsu:BSU13180.
PATRICi18974395. VBIBacSub10457_1390.

Phylogenomic databases

eggNOGiENOG4105DSS. Bacteria.
COG0620. LUCA.
HOGENOMiHOG000246221.
InParanoidiP80877.
KOiK00549.
OMAiRFGWVQS.
PhylomeDBiP80877.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BioCyciBSUB:BSU13180-MONOMER.
MetaCyc:MONOMER-14559.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETE_BACSU
AccessioniPrimary (citable) accession number: P80877
Secondary accession number(s): O34386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 16, 2009
Last modified: October 5, 2016
This is version 128 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.