P80877 (METE_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Superoxide-inducible protein 9
Short name=SOI9

Gene names

Name:	metE
Synonyms:	metC
Ordered Locus Names:	BSU13180

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Induction	By superoxide. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 762	761	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098613
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity
Experimental info
	Sequence conflict	15	1	G → D in CAA05597. Ref.1
	Sequence conflict	20	1	W → Q AA sequence Ref.4
	Sequence conflict	302	1	R → S in CAA05597. Ref.1
	Sequence conflict	309	1	I → V in CAA05597. Ref.1
	Sequence conflict	328	1	H → D in CAA05597. Ref.1
	Sequence conflict	446	1	S → R in CAA05597. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P80877 [UniParc]. Last modified June 16, 2009. Version 4. Checksum: BB85B09E6C1179EF	FASTA	762	86,806
	10 20 30 40 50 60 MTTIKTSNLG FPRIGLNREW KKALEAYWKG STDKDTFLKQ IDELFLSAVK TQIDQQIDVV 70 80 90 100 110 120 PVSDFTQYDH VLDTAVSFNW IPKRFRHLTD ATDTYFAIAR GIKDAVSSEM TKWFNTNYHY 130 140 150 160 170 180 IVPEYDESIE FRLTRNKQLE DYRRIKQEYG VETKPVIVGP YTFVTLAKGY EPSEAKAIQK 190 200 210 220 230 240 RLVPLYVQLL KELEEEGVKW VQIDEPALVT ASSEDVRGAK ELFESITSEL SSLNVLLQTY 250 260 270 280 290 300 FDSVDAYEEL ISYPVQGIGL DFVHDKGRNL EQLKTHGFPT DKVLAAGVID GRNIWKADLE 310 320 330 340 350 360 ERLDAVLDIL SIAKVDELWI QPSSSLLHVP VAKHPDEHLE KDLLNGLSYA KEKLAELTAL 370 380 390 400 410 420 KEGLVSGKAA ISEEIQQAKA DIQALKQFAT GANSEQKKEL EQLTDKDFKR PIPFEERLAL 430 440 450 460 470 480 QNESLGLPLL PTTTIGSFPQ SAEVRSARQK WRKAEWSDEQ YQNFINAETK RWIDIQEELE 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE KLAGFAFTKY AWVQSYGSRC VRPPVIYGDV EFIEPMTVKD 550 560 570 580 590 600 TVYAQSLTSK HVKGMLTGPV TILNWSFPRN DISRKEIAFQ IGLALRKEVK ALEDAGIQII 610 620 630 640 650 660 QVDEPALREG LPLKTRDWDE YLTWAAEAFR LTTSSVKNET QIHTHMCYSN FEDIVDTIND 670 680 690 700 710 720 LDADVITIEH SRSHGGFLDY LKNHPYLKGL GLGVYDIHSP RVPSTEEMYN IIVDALAVCP 730 740 750 760 TDRFWVNPDC GLKTRQQEET VAALKNMVEA AKQARAQQTQ LV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the Bacillus subtilis genome between xlyA and ykoR." Devine K.M. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 15; 302; 309; 328 AND 446.
[4]	"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis." Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M. Electrophoresis 18:1451-1463(1997) [PubMed: 9298659] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-24. Strain: 168 / IS58.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ002571 Genomic DNA. Translation: CAA05597.1. AL009126 Genomic DNA. Translation: CAB13175.2.
PIR	C69657.
RefSeq	NP_389201.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P80877.
SMR	P80877. Positions 2-758.
ModBase	Search...
Protein-protein interaction databases
IntAct	P80877. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003928; EBBACP00000003928; EBBACG00000003920.
GeneID	936480.
GenomeReviews	Gene locus BSU13180 in contig AL009126_GR.
KEGG	bsu:BSU13180.
NMPDR	fig|224308.1.peg.1320.
PATRIC	18974395. VBIBacSub10457_1390.
Organism-specific databases
GenoList	BSU13180. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001686.
HOGENOM	HBG287495.
PhylomeDB	P80877.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BSUB:BSU13180-MONOMER. MetaCyc:MONOMER-14559.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACSU

Accession

Primary (citable) accession number: P80877
Secondary accession number(s): O34386

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 99 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program