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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi645ZincBy similarity1
Metal bindingi647ZincBy similarity1
Metal bindingi730ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU13180-MONOMER
MetaCyc:MONOMER-14559
UniPathwayiUPA00051; UER00082

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Superoxide-inducible protein 9
Short name:
SOI9
Gene namesi
Name:metE
Synonyms:metC
Ordered Locus Names:BSU13180
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000986132 – 7625-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST761

Proteomic databases

PaxDbiP80877
PRIDEiP80877

Expressioni

Inductioni

By superoxide.

Interactioni

Protein-protein interaction databases

IntActiP80877, 2 interactors
MINTiP80877
STRINGi224308.Bsubs1_010100007301

Structurei

3D structure databases

ProteinModelPortaliP80877
SMRiP80877
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DSS Bacteria
COG0620 LUCA
HOGENOMiHOG000246221
InParanoidiP80877
KOiK00549
OMAiGRNIWKN
PhylomeDBiP80877

Family and domain databases

Gene3Di3.20.20.210, 4 hits
HAMAPiMF_00172 Meth_synth, 1 hit
InterProiView protein in InterPro
IPR013215 Cbl-indep_Met_Synth_N
IPR006276 Cobalamin-indep_Met_synthase
IPR002629 Met_Synth_C/arc
IPR038071 UROD/MetE-like_sf
PfamiView protein in Pfam
PF08267 Meth_synt_1, 1 hit
PF01717 Meth_synt_2, 1 hit
PIRSFiPIRSF000382 MeTrfase_B12_ind, 1 hit
SUPFAMiSSF51726 SSF51726, 2 hits
TIGRFAMsiTIGR01371 met_syn_B12ind, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTIKTSNLG FPRIGLNREW KKALEAYWKG STDKDTFLKQ IDELFLSAVK
60 70 80 90 100
TQIDQQIDVV PVSDFTQYDH VLDTAVSFNW IPKRFRHLTD ATDTYFAIAR
110 120 130 140 150
GIKDAVSSEM TKWFNTNYHY IVPEYDESIE FRLTRNKQLE DYRRIKQEYG
160 170 180 190 200
VETKPVIVGP YTFVTLAKGY EPSEAKAIQK RLVPLYVQLL KELEEEGVKW
210 220 230 240 250
VQIDEPALVT ASSEDVRGAK ELFESITSEL SSLNVLLQTY FDSVDAYEEL
260 270 280 290 300
ISYPVQGIGL DFVHDKGRNL EQLKTHGFPT DKVLAAGVID GRNIWKADLE
310 320 330 340 350
ERLDAVLDIL SIAKVDELWI QPSSSLLHVP VAKHPDEHLE KDLLNGLSYA
360 370 380 390 400
KEKLAELTAL KEGLVSGKAA ISEEIQQAKA DIQALKQFAT GANSEQKKEL
410 420 430 440 450
EQLTDKDFKR PIPFEERLAL QNESLGLPLL PTTTIGSFPQ SAEVRSARQK
460 470 480 490 500
WRKAEWSDEQ YQNFINAETK RWIDIQEELE LDVLVHGEFE RTDMVEYFGE
510 520 530 540 550
KLAGFAFTKY AWVQSYGSRC VRPPVIYGDV EFIEPMTVKD TVYAQSLTSK
560 570 580 590 600
HVKGMLTGPV TILNWSFPRN DISRKEIAFQ IGLALRKEVK ALEDAGIQII
610 620 630 640 650
QVDEPALREG LPLKTRDWDE YLTWAAEAFR LTTSSVKNET QIHTHMCYSN
660 670 680 690 700
FEDIVDTIND LDADVITIEH SRSHGGFLDY LKNHPYLKGL GLGVYDIHSP
710 720 730 740 750
RVPSTEEMYN IIVDALAVCP TDRFWVNPDC GLKTRQQEET VAALKNMVEA
760
AKQARAQQTQ LV
Length:762
Mass (Da):86,806
Last modified:June 16, 2009 - v4
Checksum:iBB85B09E6C1179EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15G → D in CAA05597 (Ref. 1) Curated1
Sequence conflicti20W → Q AA sequence (PubMed:9298659).Curated1
Sequence conflicti302R → S in CAA05597 (Ref. 1) Curated1
Sequence conflicti309I → V in CAA05597 (Ref. 1) Curated1
Sequence conflicti328H → D in CAA05597 (Ref. 1) Curated1
Sequence conflicti446S → R in CAA05597 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA Translation: CAA05597.1
AL009126 Genomic DNA Translation: CAB13175.2
PIRiC69657
RefSeqiNP_389201.2, NC_000964.3
WP_003232565.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13175; CAB13175; BSU13180
GeneIDi936480
KEGGibsu:BSU13180
PATRICifig|224308.179.peg.1431

Similar proteinsi

Entry informationi

Entry nameiMETE_BACSU
AccessioniPrimary (citable) accession number: P80877
Secondary accession number(s): O34386
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 132 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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