Reviewed, UniProtKB/Swiss-Prot P80869 (DHG2_BACSU)

Names and origin

Protein names

Recommended name:
    Glucose 1-dehydrogenase 2
    EC=1.1.1.47
Alternative name(s):
    GLCDH-II
      Short name=GDH-II
    General stress protein 74
      Short name=GSP74

Gene names

Name:	ycdF
Ordered Locus Names:	BSU02830

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.
Subunit structure	Homotetramer By similarity.
Induction	By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Stress response
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose 1-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Glucose 1-dehydrogenase 2		PRO_0000054617
Regions
	Nucleotide binding	11 – 35	25	NADP By similarity
Sites
	Active site	159	1	Proton acceptor By similarity
	Binding site	146	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80869-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 77842DC45D496C26	FASTA	258	27,776
	10 20 30 40 50 60 MYKDLTGKTA IVTGSSKGIG KAIAERFGKE KMNVVVNYHS DPSGADETLE IIKQNGGKAV 70 80 90 100 110 120 SVEADVSKEE GIQALLDTAL EHFGTLDVMV NNSGFNGVEA MPHEMSLEDW QRVIDVNVTG 130 140 150 160 170 180 TFLGAKAALN HMMKNNIKGN VLNISSVHQQ IPRPVNVQYS TSKGGIKMMT ETLALNYADK 190 200 210 220 230 240 GIRVNAIAPG TIATESNVDT KKEESRQKQL KKIPMKAFGK PEEVAAAAAW LVSEEASYVT 250 GATLFVDGGM TLYPSQLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 32 kb nucleotide sequence from the region of the lincomycin-resistance gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and identification of the site of the lin-2 mutation." Kumano M., Tamakoshi A., Yamane K. Microbiology 143:2775-2782(1997) [PubMed: 9274031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis." Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M. Electrophoresis 18:1451-1463(1997) [PubMed: 9298659] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-13. Strain: 168 / IS58.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB000617 Genomic DNA. Translation: BAA22244.1. AL009126 Genomic DNA. Translation: CAB12077.1.
PIR	G69755.
RefSeq	NP_388165.1.
3D structure databases
HSSP	HSSP built from PDB template 1GEE based on UniProtKB P40288.
ModBase	Search...
Genome annotation databases
GeneID	938377.
GenomeReviews	Gene locus BSU02830 in contig AL009126_GR.
KEGG	bsu:BSU02830.
NMPDR	fig|224308.1.peg.284.
Organism-specific databases
SubtiList	BG12761. ycdF. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P80869.
OMA	NISSVHQ.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0284-MON.
BRENDA	1.1.1.47. 150.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHG2_BACSU

Accession

Primary (citable) accession number: P80869

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1999
Last modified:	November 3, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)