Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiol peroxidase

Gene

tpx

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.UniRule annotation

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.UniRule annotation

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60Cysteine sulfenic acid (-SOH) intermediateUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciBSUB:BSU29490-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol peroxidaseUniRule annotation (EC:1.11.1.15UniRule annotation)
Short name:
TpxUniRule annotation
Alternative name(s):
Peroxiredoxin tpxUniRule annotation
Short name:
PrxUniRule annotation
Thioredoxin peroxidaseUniRule annotation
Gene namesi
Name:tpxUniRule annotation
Synonyms:ytgI
Ordered Locus Names:BSU29490
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001878702 – 167Thiol peroxidaseAdd BLAST166

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 94Redox activeCombined sources1 Publication
Disulfide bondi60 ↔ 94Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP80864.
PRIDEiP80864.

Expressioni

Inductioni

By superoxide.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP80864. 1 interactor.
MINTiMINT-8366928.
STRINGi224308.Bsubs1_010100016091.

Structurei

Secondary structure

1167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Beta strandi9 – 11Combined sources3
Beta strandi13 – 15Combined sources3
Beta strandi28 – 31Combined sources4
Beta strandi36 – 38Combined sources3
Helixi39 – 42Combined sources4
Beta strandi47 – 51Combined sources5
Beta strandi55 – 58Combined sources4
Helixi59 – 62Combined sources4
Helixi64 – 74Combined sources11
Beta strandi78 – 83Combined sources6
Helixi87 – 89Combined sources3
Helixi94 – 96Combined sources3
Beta strandi102 – 106Combined sources5
Helixi107 – 109Combined sources3
Helixi112 – 116Combined sources5
Turni122 – 124Combined sources3
Beta strandi130 – 134Combined sources5
Beta strandi140 – 145Combined sources6
Helixi156 – 166Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JSYNMR-A1-167[»]
2JSZNMR-A1-167[»]
ProteinModelPortaliP80864.
SMRiP80864.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80864.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 167ThioredoxinUniRule annotationAdd BLAST150

Sequence similaritiesi

Belongs to the peroxiredoxin family. Tpx subfamily.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4108V1J. Bacteria.
COG2077. LUCA.
HOGENOMiHOG000022345.
InParanoidiP80864.
KOiK11065.
OMAiKFNAQAN.
PhylomeDBiP80864.

Family and domain databases

CDDicd03014. PRX_Atyp2cys. 1 hit.
HAMAPiMF_00269. Tpx. 1 hit.
InterProiView protein in InterPro
IPR013740. Redoxin.
IPR036249. Thioredoxin-like_sf.
IPR013766. Thioredoxin_domain.
IPR002065. TPX.
IPR018219. Tpx_CS.
PfamiView protein in Pfam
PF08534. Redoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.
PS01265. TPX. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEITFKGGP VTLVGQEVKV GDQAPDFTVL TNSLEEKSLA DMKGKVTIIS
60 70 80 90 100
VIPSIDTGVC DAQTRRFNEE AAKLGDVNVY TISADLPFAQ ARWCGANGID
110 120 130 140 150
KVETLSDHRD MSFGEAFGVY IKELRLLARS VFVLDENGKV VYAEYVSEAT
160
NHPNYEKPIE AAKALVK
Length:167
Mass (Da):18,216
Last modified:January 23, 2007 - v3
Checksum:i5B2BD8169C0555C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00316.1.
AL009126 Genomic DNA. Translation: CAB14927.1.
PIRiF69992.
RefSeqiNP_390827.1. NC_000964.3.
WP_003223506.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14927; CAB14927; BSU29490.
GeneIDi937688.
KEGGibsu:BSU29490.
PATRICifig|224308.179.peg.3204.

Similar proteinsi

Entry informationi

Entry nameiTPX_BACSU
AccessioniPrimary (citable) accession number: P80864
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 124 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families