Reviewed, UniProtKB/Swiss-Prot P80862 (SERC_BACSU)

Names and origin

Protein names

Recommended name:
    Phosphoserine aminotransferase
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase
      Short name=PSAT
    Vegetative protein 234
      Short name=VEG234

Gene names

Name:	serC
Synonyms:	yhaF
Ordered Locus Names:	BSU10020

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity. HAMAP MF_00160
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00160
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP MF_00160
Subunit structure	Homodimer By similarity. HAMAP MF_00160
Subcellular location	Cytoplasm By similarity HAMAP MF_00160.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.
Caution	According to Ref.4, serC is not involved in pyridoxine biosynthesis in B.subtilis. In this organism, pyridoxal phosphate biosynthesis is achieved via an alternative pathway involving pdxS and pdxT.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Phosphoserine aminotransferase HAMAP MF_00160		PRO_0000150153
Regions
	Region	76 – 77	2	Pyridoxal phosphate binding By similarity
	Region	235 – 236	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	42	1	L-glutamate By similarity
	Binding site	102	1	Pyridoxal phosphate By similarity
	Binding site	151	1	Pyridoxal phosphate By similarity
	Binding site	170	1	Pyridoxal phosphate By similarity
	Binding site	193	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	194	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	5	1	T → W AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P80862-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 29EEEF609F1EFF9D	FASTA	359	40,136
	10 20 30 40 50 60 MERTTNFNAG PAALPLEVLQ KAQKEFIDFN ESGMSVMELS HRSKEYEAVH QKAKSLLIEL 70 80 90 100 110 120 MGIPEDYDIL FLQGGASLQF SMLPMNFLTP EKTAHFVMTG AWSEKALAET KLFGNTSITA 130 140 150 160 170 180 TSETDNYSYI PEVDLTDVKD GAYLHITSNN TIFGTQWQEF PNSPIPLVAD MSSDILSRKI 190 200 210 220 230 240 DVSKFDVIYG GAQKNLGPSG VTVVIMKKSW LQNENANVPK ILKYSTHVKA DSLYNTPPTF 250 260 270 280 290 300 AIYMLSLVLE WLKENGGVEA VEQRNEQKAQ VLYSCIDESN GFYKGHARKD SRSRMNVTFT 310 320 330 340 350 LRDDELTKTF VQKAKDAKMI GLGGHRSVGG CRASIYNAVS LEDCEKLAAF MKKFQQENE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene." Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S. Microbiology 144:859-875(1998) [PubMed: 9579061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis." Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M. Electrophoresis 18:1451-1463(1997) [PubMed: 9298659] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-11. Strain: 168 / IS58.
[4]	"serC is involved in vitamin B6 biosynthesis in Escherichia coli but not in Bacillus subtilis." Sakai A., Kita M., Katsuragi T., Tani Y. J. Biosci. Bioeng. 93:334-337(2002) [PubMed: 16233211] [Abstract] Cited for: NON-INVOLVEMENT IN PYRIDOXINE BIOSYNTHESIS. Strain: CRK6000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y14077 Genomic DNA. Translation: CAA74411.1. AL009126 Genomic DNA. Translation: CAB12842.1.
PIR	D69705.
RefSeq	NP_388883.1.
3D structure databases
SMR	P80862. Positions 6-356.
ModBase	Search...
Genome annotation databases
GeneID	936304.
GenomeReviews	Gene locus BSU10020 in contig AL009126_GR.
KEGG	bsu:BSU10020.
NMPDR	fig|224308.1.peg.1002.
Organism-specific databases
SubtiList	BG12673. serC. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289982.
OMA	TFAWYLA.
PhylomeDB	P80862.
Enzyme and pathway databases
BRENDA	2.6.1.52. 150.
Family and domain databases
HAMAP	MF_00160. SerC_aminotrans_5. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR003248. Pser_amintransf. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_BACSU

Accession

Primary (citable) accession number: P80862
Secondary accession number(s): O07514

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	February 9, 2010
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)