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P80860 (G6PI_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate isomerase

Short name=GPI
EC=5.3.1.9
Alternative name(s):
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI
Vegetative protein 54
Short name=VEG54
Gene names
Name:pgi
Synonyms:yugL
Ordered Locus Names:BSU31350
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP-Rule MF_00473

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP-Rule MF_00473

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00473

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00473.

Sequence similarities

Belongs to the GPI family.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucose-6-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 450449Glucose-6-phosphate isomerase HAMAP-Rule MF_00473
PRO_0000180593

Sites

Active site2901Proton donor By similarity
Active site3111 By similarity
Active site4251 By similarity

Amino acid modifications

Modified residue381Phosphothreonine Ref.5

Experimental info

Sequence conflict6 – 94FDYS → LTTP in CAB07930. Ref.1
Sequence conflict161N → PT in CAB07930. Ref.1
Sequence conflict631K → Q in CAB07930. Ref.1
Sequence conflict701K → Q in CAB07930. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P80860 [UniParc].

Last modified May 26, 2009. Version 4.
Checksum: DAAA87AFB81E1DA0

FASTA45050,543
        10         20         30         40         50         60 
MTHVRFDYSK ALTFFNEHEL TYLRDFVKTA HHNIHEKTGA GSDFLGWVDL PEHYDKEEFA 

        70         80         90        100        110        120 
RIKKSAEKIK SDSDVLLVVG IGGSYLGARA AIEALNHAFY NTLPKAKRGN PQVIFIGNNI 

       130        140        150        160        170        180 
SSSYMRDVMD LLEDVDFSIN VISKSGTTTE PAIAFRIFRK LLEEKYGKEE AKARIYATTD 

       190        200        210        220        230        240 
KERGALKTLS NEEGFESFVI PDDVGGRYSV LTAVGLLPIA VSGVNIDDMM KGALDASKDF 

       250        260        270        280        290        300 
ATSELEDNPA YQYAVVRNVL YNKGKTIEML INYEPALQYF AEWWKQLFGE SEGKDEKGIY 

       310        320        330        340        350        360 
PSSANYSTDL HSLGQYVQEG RRDLFETVLN VEKPKHELTI EEADNDLDGL NYLAGKTVDF 

       370        380        390        400        410        420 
VNKKAFQGTM LAHTDGNVPN LIVNIPELNA YTFGYLVYFF EKACAMSGYL LGVNPFDQPG 

       430        440        450 
VEAYKVNMFA LLGKPGFEEK KAELEKRLED 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 6-9; 16; 63 AND 70.
[4]"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
Electrophoresis 18:1451-1463(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11.
Strain: 168 / IS58.
[5]"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis."
Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M.
Mol. Cell. Proteomics 6:697-707(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-38, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z93936 Genomic DNA. Translation: CAB07930.1.
AL009126 Genomic DNA. Translation: CAB15124.2.
PIRB69675.
RefSeqNP_391013.2. NC_000964.3.

3D structure databases

ProteinModelPortalP80860.
SMRP80860. Positions 2-449.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP80860. 1 interaction.
MINTMINT-8366174.
STRING224308.BSU31350.

PTM databases

PhosSiteP0802227.

Proteomic databases

PaxDbP80860.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15124; CAB15124; BSU31350.
GeneID937165.
KEGGbsu:BSU31350.
PATRIC18978238. VBIBacSub10457_3282.

Organism-specific databases

GenoListBSU31350. [Micado]

Phylogenomic databases

eggNOGCOG0166.
HOGENOMHOG000100403.
KOK01810.
OrthoDBEOG64R61J.
PhylomeDBP80860.

Enzyme and pathway databases

BioCycBSUB:BSU31350-MONOMER.
UniPathwayUPA00109; UER00181.

Family and domain databases

HAMAPMF_00473. G6P_isomerase.
InterProIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERPTHR11469. PTHR11469. 1 hit.
PfamPF00342. PGI. 1 hit.
[Graphical view]
PRINTSPR00662. G6PISOMERASE.
PROSITEPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PI_BACSU
AccessionPrimary (citable) accession number: P80860
Secondary accession number(s): O08330
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 26, 2009
Last modified: July 9, 2014
This is version 105 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList