Reviewed, UniProtKB/Swiss-Prot P80750 (RIPX_CUCPE)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Cucurbita pepo (Vegetable marrow) (Summer squash)
Taxonomic identifier	3663 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Cucurbita

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	Ribosome-inactivating protein		PRO_0000221399
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P80750-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8E753D217FDEAA6D	FASTA	20	2,235
	10 20 NVRFDLSGAT SSSYKTFIKN

References

[1]

"Purification, characterization and subcellular localization of a type-1 ribosome-inactivating protein from the sarcocarp of Cucurbita pepo." Yoshinari S., Yokota S., Sawmoto H., Koresawa S., Tamura M., Endo Y. Eur. J. Biochem. 242:585-591(1996) [PubMed: 9022685] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Mesocarp.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 1943.
Family and domain databases
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIPX_CUCPE

Accession

Primary (citable) accession number: P80750

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)