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P80725 (DPS_LISIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA protection during starvation protein

EC=1.16.-.-
Alternative name(s):
Ferritin-like protein
Non-heme iron-containing ferritin
Gene names
Name:dps
Synonyms:flp, fri
Ordered Locus Names:lin0942
OrganismListeria innocua serovar 6a (strain CLIP 11262) [Complete proteome] [HAMAP]
Taxonomic identifier272626 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length156 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction. Does not bind DNA. Ref.1 Ref.2 Ref.4

Catalytic activity

2 Fe2+ + H2O2 + 2 H+ = 2 Fe3+ + 2 H2O.

Subunit structure

Homododecamer. The 12 subunits form a hollow sphere into which the mineral iron core of up to 500 Fe3+ can be deposited. Ref.5

Subcellular location

Cytoplasm By similarity.

Induction

By iron limitation and stationary growth phase. Ref.2

Domain

12 di-nuclear ferroxidase centers are located at the interfaces between subunits related by 2-fold symmetry axes.

Sequence similarities

Belongs to the Dps family.

Ontologies

Keywords
   Biological processIron storage
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcellular iron ion homeostasis

Inferred from electronic annotation. Source: UniProtKB-KW

response to stress

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionferric iron binding

Inferred from electronic annotation. Source: InterPro

oxidoreductase activity, oxidizing metal ions

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 156156DNA protection during starvation protein
PRO_0000201657

Sites

Metal binding311Iron 1; shared with dodecameric partner
Metal binding581Iron 1
Metal binding621Iron 1
Metal binding621Iron 2 Probable

Experimental info

Mutagenesis311H → G: Slight decrease in DNA protection and significant decrease in iron affinity. Retains only one third of wild-type DNA protection and loses iron binding ability; when associated with G-43. Ref.6
Mutagenesis431H → G: Slight decrease in DNA protection and significant decrease iron affinity. Retains only one third of wild-type DNA protection and loses iron-binding ability; when associated with G-31. Ref.6
Sequence conflict631R → L Ref.2

Secondary structure

........... 156
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P80725 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 5FC9FFF5EE7FB6F8

FASTA15618,049
        10         20         30         40         50         60 
MKTINSVDTK EFLNHQVANL NVFTVKIHQI HWYMRGHNFF TLHEKMDDLY SEFGEQMDEV 

        70         80         90        100        110        120 
AERLLAIGGS PFSTLKEFLE NASVEEAPYT KPKTMDQLME DLVGTLELLR DEYKQGIELT 

       130        140        150 
DKEGDDVTND MLIAFKASID KHIWMFKAFL GKAPLE 

« Hide

References

« Hide 'large scale' references
[1]"A novel non-heme iron-binding ferritin related to the DNA-binding proteins of the Dps family in Listeria innocua."
Bozzi M., Mignogna G., Stefanini S., Barra D., Longhi C., Valenti P., Chiancone E.
J. Biol. Chem. 272:3259-3265(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION.
[2]"The expression of the dodecameric ferritin in Listeria spp. is induced by iron limitation and stationary growth phase."
Polidoro M., De Biase D., Montagnini B., Guarrera L., Cavallo S., Valenti P., Stefanini S., Chiancone E.
Gene 296:121-128(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
Strain: LS1.
[3]"Comparative genomics of Listeria species."
Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. expand/collapse author list , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.
Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CLIP 11262.
[4]"The so-called Listeria innocua ferritin is a Dps protein. Iron incorporation, detoxification, and DNA protection properties."
Su M., Cavallo S., Stefanini S., Chiancone E., Chasteen N.D.
Biochemistry 44:5572-5578(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN DNA PROTECTION, IRON INCORPORATION.
[5]"The dodecameric ferritin from Listeria innocua contains a novel intersubunit iron-binding site."
Ilari A., Stefanini S., Chiancone E., Tsernoglou D.
Nat. Struct. Biol. 7:38-43(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH IRON, SUBUNIT.
[6]"The unusual intersubunit ferroxidase center of Listeria innocua Dps is required for hydrogen peroxide detoxification but not for iron uptake. A study with site-specific mutants."
Ilari A., Latella M.C., Ceci P., Ribacchi F., Su M., Giangiacomo L., Stefanini S., Chasteen N.D., Chiancone E.
Biochemistry 44:5579-5587(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.19 ANGSTROMS) OF MUTANTS GLY-31 AND GLY-43, MUTAGENESIS OF HIS-31 AND HIS-43.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ244014 Genomic DNA. Translation: CAB65175.2.
AL596167 Genomic DNA. Translation: CAC96173.1.
PIRAE1550.
RefSeqNP_470279.1. NC_003212.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1QGHX-ray2.35A/B/C/D/E/F/G/H/I/J/K/L1-156[»]
2BJYX-ray2.60A/B/C/D/E/F/G/H/I/J/K/L1-156[»]
2BK6X-ray2.19A/B/C/D/E/F1-156[»]
2BKCX-ray2.30A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/X/Y1-156[»]
ProteinModelPortalP80725.
SMRP80725. Positions 7-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272626.lin0942.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC96173; CAC96173; CAC96173.
GeneID1129452.
KEGGlin:lin0942.
PATRIC20298599. VBILisInn102668_0967.

Organism-specific databases

GenoListLIN0942.
CMRSearch...

Phylogenomic databases

eggNOGCOG0783.
HOGENOMHOG000273542.
KOK04047.
OMAVEGICEM.
OrthoDBEOG6Z9B6G.

Family and domain databases

Gene3D1.20.1260.10. 1 hit.
InterProIPR002177. DPS_DNA-bd.
IPR023188. DPS_DNA-bd_CS.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFPIRSF005900. Dps. 1 hit.
PRINTSPR01346. HELNAPAPROT.
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00818. DPS_1. 1 hit.
PS00819. DPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP80725.

Entry information

Entry nameDPS_LISIN
AccessionPrimary (citable) accession number: P80725
Secondary accession number(s): Q9RE06
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references