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Protein

Potassium channel toxin alpha-KTx 6.2

Gene
N/A
Organism
Scorpio palmatus (Israeli golden scorpion) (Scorpio maurus palmatus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Blocks voltage-gated potassium channels (Shaker B, and Kv1.2/KCNA2 (IC50=0.8 nM)) and is also active on Kv1.1/KCNA1 (IC50=45 nM) and Kv1.3/KCNA3 (IC50=180 nM). Also inhibits apamin-sensitive small conductance calcium-activated potassium (SK/KCNN) channels, and inhibits intermediate conductance calcium-activated potassium KCa3.1/KCNN4 channels (IC50=1 nM).6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei23Basic residue of the functional dyad, critical for activity1
Sitei32Aromatic residue of the functional dyadBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 6.2
Alternative name(s):
Maurotoxin
Short name:
MTX
OrganismiScorpio palmatus (Israeli golden scorpion) (Scorpio maurus palmatus)
Taxonomic identifieri1662106 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaScorpionoideaScorpionidaeScorpioninaeScorpio

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 4 µg/kg by intracerebroventricular injection into mice.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15K → Q: Induces a change in the third and fourth disulfide bonds, leading to disulfide bonds between C-13 and C-31, and C-19 and C-34. 1 Publication1
Mutagenesisi23K → A: 1000-fold decrease in affinity. 1 Publication1
Mutagenesisi33G → A: Induces a change in the third and fourth disulfide bonds, leading to disulfide bonds between C-13 and C-31, and C-19 and C-34. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000449291 – 34Potassium channel toxin alpha-KTx 6.2Add BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi3 ↔ 245 Publications
Disulfide bondi9 ↔ 295 Publications
Disulfide bondi13 ↔ 195 Publications
Disulfide bondi31 ↔ 345 Publications
Modified residuei34Cysteine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 16Combined sources11
Beta strandi22 – 25Combined sources4
Beta strandi28 – 31Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TXMNMR-A1-34[»]
1WPDNMR-A1-16[»]
1WT7NMR-A3-34[»]
ProteinModelPortaliP80719.
SMRiP80719.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80719.

Family & Domainsi

Domaini

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiView protein in InterPro
IPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
PfamiView protein in Pfam
PF00451. Toxin_2. 1 hit.
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD003586. Scorpion_toxinS. 1 hit.
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiView protein in PROSITE
PS01138. SCORP_SHORT_TOXIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P80719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
VSCTGSKDCY APCRKQTGCP NAKCINKSCK CYGC
Length:34
Mass (Da):3,621
Last modified:November 1, 1997 - v1
Checksum:iFB0BE07370536A48
GO

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiKAX62_SCOPA
AccessioniPrimary (citable) accession number: P80719
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 15, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Caution

Has not the same disulfide pairing as other members of alpha-KTx 6.5 Publications

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  3. SIMILARITY comments
    Index of protein domains and families