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Protein

Potassium channel toxin alpha-KTx 6.2

Gene
N/A
Organism
Scorpio maurus palmatus (Chactoid scorpion)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Blocks voltage-gated potassium channels (Shaker B, and Kv1.2/KCNA2 (IC50=0.8 nM)) and is also active on Kv1.1/KCNA1 (IC50=45 nM) and Kv1.3/KCNA3 (IC50=180 nM). Also inhibits apamin-sensitive small conductance calcium-activated potassium (SK/KCNN) channels, and inhibits intermediate conductance calcium-activated potassium KCa3.1/KCNN4 channels (IC50=1 nM).6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei23Basic residue of the functional dyad, critical for activity1
Sitei32Aromatic residue of the functional dyadBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 6.2
Alternative name(s):
Maurotoxin
Short name:
MTX
OrganismiScorpio maurus palmatus (Chactoid scorpion)
Taxonomic identifieri1662106 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaScorpionoideaScorpionidaeScorpioninaeScorpio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 4 µg/kg by intracerebroventricular injection into mice.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15K → Q: Induces a change in the third and fourth disulfide bonds, leading to disulfide bonds between C-13 and C-31, and C-19 and C-34. 1 Publication1
Mutagenesisi23K → A: 1000-fold decrease in affinity. 1 Publication1
Mutagenesisi33G → A: Induces a change in the third and fourth disulfide bonds, leading to disulfide bonds between C-13 and C-31, and C-19 and C-34. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000449291 – 34Potassium channel toxin alpha-KTx 6.2Add BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi3 ↔ 245 Publications
Disulfide bondi9 ↔ 295 Publications
Disulfide bondi13 ↔ 195 Publications
Disulfide bondi31 ↔ 345 Publications
Modified residuei34Cysteine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 16Combined sources11
Beta strandi22 – 25Combined sources4
Beta strandi28 – 31Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TXMNMR-A1-34[»]
1WPDNMR-A1-16[»]
1WT7NMR-A3-34[»]
ProteinModelPortaliP80719.
SMRiP80719.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80719.

Family & Domainsi

Domaini

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
VSCTGSKDCY APCRKQTGCP NAKCINKSCK CYGC
Length:34
Mass (Da):3,621
Last modified:November 1, 1997 - v1
Checksum:iFB0BE07370536A48
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TXMNMR-A1-34[»]
1WPDNMR-A1-16[»]
1WT7NMR-A3-34[»]
ProteinModelPortaliP80719.
SMRiP80719.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP80719.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAX62_SCOMA
AccessioniPrimary (citable) accession number: P80719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Caution

Has not the same disulfide pairing as other members of alpha-KTx 6.5 Publications

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.