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P80703

- APL3_GALME

UniProt

P80703 - APL3_GALME

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Protein

Apolipophorin-3

Gene
N/A
Organism
Galleria mellonella (Greater wax moth)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Assists in the loading of diacylglycerol, generated from triacylglycerol stores in the fat body through the action of adipokinetic hormone, into lipophorin, the hemolymph lipoprotein. It increases the lipid carrying capacity of lipophorin by covering the expanding hydrophobic surface resulting from diacylglycerol uptake. It thus plays a critical role in the transport of lipids during flight in several species of insects. Has antibacterial activity against the Gram-positive bacteria L.monocytogenes (MIC=6.5 µM). Lacks antibacterial activity against the Gram-positive bacteria B.circulans, M.luteus, S.aureus, and S.lutea, and the Gram-negative bacteria E.coli D31, E.coli ATCC 25922, and S.typhimurium. Lacks antifungal activity against S.cerevisiae, P.pastoris, Z.marxianus, C.albicans, C.wickerhamii, A.niger, F.oxysporum, and T.harizianum.1 Publication

GO - Molecular functioni

  1. lipid binding Source: InterPro

GO - Biological processi

  1. defense response to Gram-positive bacterium Source: UniProtKB
  2. innate immune response Source: UniProtKB
  3. lipid transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Keywords - Biological processi

Immunity, Innate immunity, Lipid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipophorin-3
Alternative name(s):
Apolipophorin-III
Short name:
ApoLp-III
OrganismiGalleria mellonella (Greater wax moth)
Taxonomic identifieri7137 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPyraloideaPyralidaeGalleriinaeGalleria

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei3638. Gal m 24kD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Propeptidei19 – 2351 PublicationPRO_0000002042
Chaini24 – 186163Apolipophorin-3PRO_0000002043Add
BLAST

Keywords - PTMi

Cleavage on pair of basic residues

Expressioni

Tissue specificityi

Hemolymph.1 Publication

Inductioni

By bacterial infection.1 Publication

Interactioni

Subunit structurei

Equilibrium between a soluble monomer and a bound lipoprotein form. Apolipophorin-3 associates with lipophorin during lipid loading until each particle contains 9 or 14 molecules of apolipophorin-3.

Structurei

3D structure databases

ProteinModelPortaliP80703.
SMRiP80703. Positions 29-186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insect apolipophorin-3 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR010009. ApoLp-III.
[Graphical view]
PfamiPF07464. ApoLp-III. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80703-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAKYVFVVA ACSALAQAGI VRRDASTPLQ DLEKHAAEFQ KTFSEQLNAF
60 70 80 90 100
TNSKDTKEFN TALKEGSDSV LQQLNALASS LQKALNDANG KAKEALEQTR
110 120 130 140 150
TNLERTAEEL RRAHPDVERQ AGALRDRLQT AVQATVQETQ KLAKTVGANL
160 170 180
EETNKKLAPQ IKSAYDDFVK QAQEVQKKLH EAASKQ
Length:186
Mass (Da):20,453
Last modified:December 15, 1998 - v2
Checksum:iC79B381E07F5C070
GO

Mass spectrometryi

Molecular mass is 5712.7 Da from positions 136 - 186. Determined by ESI. The measured mass may be that of a peptide that is synthesized after immune challenge or a fragment of partial proteolytic digestion.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006975 mRNA. Translation: CAA07363.1.
PIRiT09296.

Cross-referencesi

Web resourcesi

Protein Spotlight

Lipid freight - Issue 59 of June 2005

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006975 mRNA. Translation: CAA07363.1 .
PIRi T09296.

3D structure databases

ProteinModelPortali P80703.
SMRi P80703. Positions 29-186.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3638. Gal m 24kD.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR010009. ApoLp-III.
[Graphical view ]
Pfami PF07464. ApoLp-III. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Functional expression of Galleria mellonella apolipophorin III."
    Niere M., Dettloff M., Weise C., Meisslitzer C., Ziegler M., Wiesner A.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fat body.
  2. "Primary structure of apolipophorin-III from the greater wax moth, Galleria mellonella."
    Weise C., Franke P., Kopacek P., Wiesner A.
    J. Protein Chem. 17:633-641(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-186.
    Tissue: Hemolymph.
  3. "Purification and characterization of eight peptides from Galleria mellonella immune hemolymph."
    Cytrynska M., Mak P., Zdybicka-Barabas A., Suder P., Jakubowicz T.
    Peptides 28:533-546(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 136-186, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, MASS SPECTROMETRY.
    Tissue: Larval hemolymph.

Entry informationi

Entry nameiAPL3_GALME
AccessioniPrimary (citable) accession number: P80703
Secondary accession number(s): O76946
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: October 1, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3