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P80703

- APL3_GALME

UniProt

P80703 - APL3_GALME

Protein

Apolipophorin-3

Gene
N/A
Organism
Galleria mellonella (Greater wax moth)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Assists in the loading of diacylglycerol, generated from triacylglycerol stores in the fat body through the action of adipokinetic hormone, into lipophorin, the hemolymph lipoprotein. It increases the lipid carrying capacity of lipophorin by covering the expanding hydrophobic surface resulting from diacylglycerol uptake. It thus plays a critical role in the transport of lipids during flight in several species of insects. Has antibacterial activity against the Gram-positive bacteria L.monocytogenes (MIC=6.5 µM). Lacks antibacterial activity against the Gram-positive bacteria B.circulans, M.luteus, S.aureus, and S.lutea, and the Gram-negative bacteria E.coli D31, E.coli ATCC 25922, and S.typhimurium. Lacks antifungal activity against S.cerevisiae, P.pastoris, Z.marxianus, C.albicans, C.wickerhamii, A.niger, F.oxysporum, and T.harizianum.1 Publication

    GO - Molecular functioni

    1. lipid binding Source: InterPro

    GO - Biological processi

    1. defense response to Gram-positive bacterium Source: UniProtKB
    2. innate immune response Source: UniProtKB
    3. lipid transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial

    Keywords - Biological processi

    Immunity, Innate immunity, Lipid transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apolipophorin-3
    Alternative name(s):
    Apolipophorin-III
    Short name:
    ApoLp-III
    OrganismiGalleria mellonella (Greater wax moth)
    Taxonomic identifieri7137 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPyraloideaPyralidaeGalleriinaeGalleria

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Protein family/group databases

    Allergomei3638. Gal m 24kD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 2351 PublicationPRO_0000002042
    Chaini24 – 186163Apolipophorin-3PRO_0000002043Add
    BLAST

    Keywords - PTMi

    Cleavage on pair of basic residues

    Expressioni

    Tissue specificityi

    Hemolymph.1 Publication

    Inductioni

    By bacterial infection.1 Publication

    Interactioni

    Subunit structurei

    Equilibrium between a soluble monomer and a bound lipoprotein form. Apolipophorin-3 associates with lipophorin during lipid loading until each particle contains 9 or 14 molecules of apolipophorin-3.

    Structurei

    3D structure databases

    ProteinModelPortaliP80703.
    SMRiP80703. Positions 29-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the insect apolipophorin-3 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR010009. ApoLp-III.
    [Graphical view]
    PfamiPF07464. ApoLp-III. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80703-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAKYVFVVA ACSALAQAGI VRRDASTPLQ DLEKHAAEFQ KTFSEQLNAF    50
    TNSKDTKEFN TALKEGSDSV LQQLNALASS LQKALNDANG KAKEALEQTR 100
    TNLERTAEEL RRAHPDVERQ AGALRDRLQT AVQATVQETQ KLAKTVGANL 150
    EETNKKLAPQ IKSAYDDFVK QAQEVQKKLH EAASKQ 186
    Length:186
    Mass (Da):20,453
    Last modified:December 15, 1998 - v2
    Checksum:iC79B381E07F5C070
    GO

    Mass spectrometryi

    Molecular mass is 5712.7 Da from positions 136 - 186. Determined by ESI. The measured mass may be that of a peptide that is synthesized after immune challenge or a fragment of partial proteolytic digestion.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006975 mRNA. Translation: CAA07363.1.
    PIRiT09296.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Lipid freight - Issue 59 of June 2005

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006975 mRNA. Translation: CAA07363.1 .
    PIRi T09296.

    3D structure databases

    ProteinModelPortali P80703.
    SMRi P80703. Positions 29-186.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 3638. Gal m 24kD.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR010009. ApoLp-III.
    [Graphical view ]
    Pfami PF07464. ApoLp-III. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional expression of Galleria mellonella apolipophorin III."
      Niere M., Dettloff M., Weise C., Meisslitzer C., Ziegler M., Wiesner A.
      Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fat body.
    2. "Primary structure of apolipophorin-III from the greater wax moth, Galleria mellonella."
      Weise C., Franke P., Kopacek P., Wiesner A.
      J. Protein Chem. 17:633-641(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-186.
      Tissue: Hemolymph.
    3. "Purification and characterization of eight peptides from Galleria mellonella immune hemolymph."
      Cytrynska M., Mak P., Zdybicka-Barabas A., Suder P., Jakubowicz T.
      Peptides 28:533-546(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 136-186, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, MASS SPECTROMETRY.
      Tissue: Larval hemolymph.

    Entry informationi

    Entry nameiAPL3_GALME
    AccessioniPrimary (citable) accession number: P80703
    Secondary accession number(s): O76946
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3